Application of soluble CD30 as biological index for rapidly estimating ionizing radiation dosage
A technology of ionizing radiation and biological indicators, applied in the field of ionizing radiation biological dose estimation, can solve the problems of unreported correlation and achieve the effect of convenient sample preservation, less requirement and objective results
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Embodiment 1
[0024] Implementation Example 1: Establishment of Mouse Irradiation Model and Sample Collection
[0025] Experimental animals: male C57BL / 6 mice, 7 weeks old, weighing 19±1g, purchased from Shanghai Slack Experimental Animal Co., Ltd., animal quality certificate number: 2007000534826, experimental animal production license number SCXK (Shanghai) 2007- 0005.
[0026] Serum preparation: Mice were irradiated at 60 The cobalt irradiation center carried out, and 80 mice were randomly divided into four groups, 20 in each group: 1 group in the control group and 4 groups in the radiation group. The mice in the radiation group received 1Gy, 3Gy, 5Gy and 10Gy total body irradiation respectively at a dose rate of 0.78Gy / min. At 24h, 48h and 72h after irradiation, blood was collected from the mice through the eyeball. The whole blood was clotted at 37°C for 1h, centrifuged at 3000rpm×15min, and the supernatant serum was separated and distributed into centrifuge tubes at 100μl / tube. Ali...
Embodiment 2
[0027] Implementation example two: detection of sCD30 content in mouse serum
[0028] (1) Detection of sCD30 content in serum of non-irradiated mice
[0029] Testing materials and methods:
[0030] Cytokine antibody chip detection. The chip name is: Custom Mouse Array chip, customized by RayBiotech.
[0031] The chip detection operation steps are as follows:
[0032] (1) Place the chip bag at room temperature for 30 minutes, tear off the sealing film, mark the chip, and dry it on a clean bench for 2 hours;
[0033] (2) Sample preparation: 60 μl serum + 60 μl diluent, mix well;
[0034] (3) Preparation of standard substance: take 10000pg / ml standard solution (std1), three-fold dilution 6 times (std2~7), the lowest concentration is 13.7pg / ml (std7).
[0035] (4) Preparation of negative control: Take 120 μl of diluent (CNTRL)
[0036] (5) Chip blocking and sample incubation: add 100Rl blocking solution to each well, block at room temperature for 0.5 h, shake gently, add...
Embodiment 3
[0062] Implementation Example 3: Estimation of Irradiation Dose in Mice
[0063] According to the concentration of serum sAXL and the irradiation time, estimate the dose to the mice. See Table 4-5 for the estimation method.
[0064] Table 4 Estimated dose of exposure to mice from 0 to 36 hours after irradiation
[0065]
[0066] Table 5 Estimated dose of exposure to mice 36-60 hours after irradiation
[0067]
[0068] Table 6 Estimated dose of exposure to mice 60-84 hours after irradiation
[0069]
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