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Application of soluble CD30 as biological index for rapidly estimating ionizing radiation dosage

A technology of ionizing radiation and biological indicators, applied in the field of ionizing radiation biological dose estimation, can solve the problems of unreported correlation and achieve the effect of convenient sample preservation, less requirement and objective results

Inactive Publication Date: 2013-10-02
NAVY MEDICINE RES INST OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the correlation between sCD30 content and ionizing radiation dose has not been reported so far

Method used

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  • Application of soluble CD30 as biological index for rapidly estimating ionizing radiation dosage
  • Application of soluble CD30 as biological index for rapidly estimating ionizing radiation dosage
  • Application of soluble CD30 as biological index for rapidly estimating ionizing radiation dosage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Implementation Example 1: Establishment of Mouse Irradiation Model and Sample Collection

[0025] Experimental animals: male C57BL / 6 mice, 7 weeks old, weighing 19±1g, purchased from Shanghai Slack Experimental Animal Co., Ltd., animal quality certificate number: 2007000534826, experimental animal production license number SCXK (Shanghai) 2007- 0005.

[0026] Serum preparation: Mice were irradiated at 60 The cobalt irradiation center carried out, and 80 mice were randomly divided into four groups, 20 in each group: 1 group in the control group and 4 groups in the radiation group. The mice in the radiation group received 1Gy, 3Gy, 5Gy and 10Gy total body irradiation respectively at a dose rate of 0.78Gy / min. At 24h, 48h and 72h after irradiation, blood was collected from the mice through the eyeball. The whole blood was clotted at 37°C for 1h, centrifuged at 3000rpm×15min, and the supernatant serum was separated and distributed into centrifuge tubes at 100μl / tube. Ali...

Embodiment 2

[0027] Implementation example two: detection of sCD30 content in mouse serum

[0028] (1) Detection of sCD30 content in serum of non-irradiated mice

[0029] Testing materials and methods:

[0030] Cytokine antibody chip detection. The chip name is: Custom Mouse Array chip, customized by RayBiotech.

[0031] The chip detection operation steps are as follows:

[0032] (1) Place the chip bag at room temperature for 30 minutes, tear off the sealing film, mark the chip, and dry it on a clean bench for 2 hours;

[0033] (2) Sample preparation: 60 μl serum + 60 μl diluent, mix well;

[0034] (3) Preparation of standard substance: take 10000pg / ml standard solution (std1), three-fold dilution 6 times (std2~7), the lowest concentration is 13.7pg / ml (std7).

[0035] (4) Preparation of negative control: Take 120 μl of diluent (CNTRL)

[0036] (5) Chip blocking and sample incubation: add 100Rl blocking solution to each well, block at room temperature for 0.5 h, shake gently, add...

Embodiment 3

[0062] Implementation Example 3: Estimation of Irradiation Dose in Mice

[0063] According to the concentration of serum sAXL and the irradiation time, estimate the dose to the mice. See Table 4-5 for the estimation method.

[0064] Table 4 Estimated dose of exposure to mice from 0 to 36 hours after irradiation

[0065]

[0066] Table 5 Estimated dose of exposure to mice 36-60 hours after irradiation

[0067]

[0068] Table 6 Estimated dose of exposure to mice 60-84 hours after irradiation

[0069]

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Abstract

The invention belongs to a biological index for rapidly estimating the ionizing radiation dosage and particularly relates to an application of soluble CD30 (sCD30) as a biological index for rapidly estimating the ionizing radiation dosage. After radiation, the concentration of the sCD30 in blood serum or blood plasma is reduced along with the increasing of the radiation dosage; after the radiation is carried out within a certain time range, a very good dosage-effect relation exists; the rapid detection can be carried out by using a simple and feasible method through ELISA (Enzyme Linked Immunosorbent Assay), a protection chip and the like; a little amount of sample is needed by the detection and the sampling is convenient. Therefore, the sCD30 can be used as the biological index for rapidly estimating the ionizing radiation dosage; the radiation dosage of a human body and an animal after ionizing radiation is rapidly calculated by adopting the content of the sCD30 in the blood serum or the blood plasma.

Description

1 technical field [0001] The invention belongs to the field of ionizing radiation biological dose estimation. It specifically relates to the use of soluble CD30 (sCD30) as a biomarker for rapid estimation of ionizing radiation dose. 2 background technology [0002] Countries around the world are increasingly paying attention to the application of nuclear energy. my country's nuclear power is moving from "moderate development" to "active development" and will play a very important role in the future power supply. With the rapid development of science and technology, radioactive sources and radiation technology are widely used in the fields of industry and agriculture, medicine, teaching and even military affairs. However, while nuclear technology benefits mankind, it also poses a potential threat to public safety and public health. Acute radiation sickness is the epitome of this threat. Acute radiation sickness refers to a systemic disease caused by the body being exposed ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 何颖刘琼沈先荣侯登勇陈伟蒋定文刘玉明王庆蓉钱甜甜李珂娴
Owner NAVY MEDICINE RES INST OF PLA
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