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A method of simultaneously detecting the content of artesunate and the content of dihydroartemisinin in animal blood plasma

A technology for dihydroartemisinin and animal detection, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of difficulty in meeting the requirements of biological sample detection, complex detection methods, and high ultraviolet detection limits, and achieves good peak shape. , The effect of accurate content determination and short analysis time

Inactive Publication Date: 2014-07-23
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Claims
  • Application Information

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Problems solved by technology

However, due to the molecular structure of artesunate, its ultraviolet absorption is very weak, and the ultraviolet detection limit is high, so it is difficult to meet the requirements of biological sample detection, and the detection method is complicated and difficult to operate.

Method used

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  • A method of simultaneously detecting the content of artesunate and the content of dihydroartemisinin in animal blood plasma
  • A method of simultaneously detecting the content of artesunate and the content of dihydroartemisinin in animal blood plasma
  • A method of simultaneously detecting the content of artesunate and the content of dihydroartemisinin in animal blood plasma

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Embodiment

[0032] The artesunate nanoemulsion used in the present invention (the main component is artesunate, and the excipients are ethyl oleate, Tween-80, n-butanol, ultrapure water, place of origin: Lanzhou) was produced by Lanzhou Institute of Animal Husbandry and Veterinary Medicine The self-developed new anti-animal parasite veterinary drug (National Invention Patent No.: ZL 200810150303.4) is mainly used in veterinary clinics for the treatment of theilesisis of cattle and sheep with remarkable curative effect.

[0033] 1. Instrument

[0034] Agilent 1200 / 6410 Triple Quad LC / MSMS; C18 chromatographic column (Dalian Elite Company); OASiS HLB solid-phase extraction column; American Millipore water purification system; KL512 nitrogen blowing instrument (product of Beijing Kanglin Technology Co., Ltd.); Thermo Centrifuge, vortex oscillator (Heros BIO); syringe-type microporous membrane filter (product of Shanghai Xingya Purification Material Factory, pore size 0.22 μm).

[0035] 2. D...

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Abstract

The invention discloses a method of simultaneously detecting the content of artesunate and the content of dihydroartemisinin in animal blood plasma. The method comprises steps of: extracting a blood plasma sample with a solvent, processing the sample liquid with a solid phase extraction technology, adding the sample liquid into a small processed solid-phase extraction column, washing the column with acetic acid and a methanol-acetic acid solution, eluting with ethyl acetate and 1-chorobutane, collecting the eluate, blowing the eluate to dry with nitrogen, dissolving residue with methanol so as to obtain a sample solution to be tested, and accurately measuring the content of the artesunate and the content of the dihydroartemisinin in the sheep blood plasma by utilization of HPLC-MS / MS. The lowest detectable limit of the artesunate is 0.1 ng*mL<-1>. The lowest detectable limit of the dihydroartemisinin is 1 ng*mL<-1>. The method reduces influences of impurities, enriches the sample concentration and is high in specificity and good in separation effect. Linearity, stability, reproducibility, and recovery tests of the method satisfy good technical requirements. The method lays methodology foundations for research of pharmacokinetics and bioequivalence of the medicine inside animals.

Description

[0001] technical field [0002] The invention belongs to the field of pharmaceutical analysis and relates to a method for simultaneously determining the content of artesunate and dihydroartemisinin in animal plasma by using solid phase extraction pretreatment technology and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) method Methods. [0003] Background technique [0004] In Asian and African countries, artemisinin and its derivative artesunate are widely used in the treatment of malaria, especially the treatment of Plasmodium falciparum. The detection method of artesunate and its metabolite dihydroartemisinin in vivo has not been reported in China. Currently reported detection methods for the content of artesunate mainly include HPLC-UV detection, electrochemical detection, and HPLC-evaporative light detection. However, due to the molecular structure of artesunate, its ultraviolet absorption is very weak and its ultraviolet detection limit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 李冰张继瑜周绪正牛建荣魏小娟李金善李剑勇杨亚军刘希望张杰
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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