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A fully automatic microfluidic chip fluorescent immunoassay system and its detection method

A technology of microfluidic chips and fluorescent immunity, applied in fluid controllers, chemical instruments and methods, biological testing, etc., can solve the problems of not fundamentally realizing multi-sample, high-throughput, rapid detection, etc., to save repeated The effect of sampling operation, no pollution to the environment, and short detection time

Active Publication Date: 2019-02-05
沈阳优宁生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, in order to adapt to multiple samples, rapid detection, and reduce manual operations, various fully automatic microfluidic detectors have been introduced one after another. However, these automated instruments only reduce manual operations, liberate labor, eliminate subjective errors in experiments, and There is no fundamental requirement for multi-sample, high-throughput, and rapid detection

Method used

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  • A fully automatic microfluidic chip fluorescent immunoassay system and its detection method
  • A fully automatic microfluidic chip fluorescent immunoassay system and its detection method
  • A fully automatic microfluidic chip fluorescent immunoassay system and its detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0047] As shown in the figure, a fully automatic microfluidic chip fluorescent immunoassay system includes a housing 1 and a main frame 2, the upper part of the main frame 2 is provided with a chip card storage disk 3 and a sample tube disk 5, the chip card storage disk 3 and the sample A centrifugal reaction disk 4 is arranged between the tube trays 5, a filling system 7 and a cleaning system 8 are arranged between the centrifugal reaction disk 4 and the sample tube tray 5; an optical detection system 9 is arranged on the main frame 2 corresponding to the centrifugal reaction disk 4 , the inside of the main frame 2 is provided with a control system and a motor 10, the motor 10 drives the rotation of the chip card storage disk 3, the centrifugal reaction disk 4, the sample tube disk 5, and the filling system 7.

[0048] The chip card storage disk 3, the centrifugal reaction disk 4, the sample tube disk 5, and the filling system 7 are respectively connected to the drive motor 10...

Embodiment 2

[0058] 8 kinds of chip detection cards were made for 8 common tumor marker antigens (① alpha-fetoprotein AFP, ② carcinoembryonic antigen CEA, ③ total prostate-specific antigen t-PSA, ④ free prostate-specific antigen f-PSA, ⑤ squamous Epithelial cell carcinoma antigen SCC, ⑥ glycogen antigen 125, ⑦ glycogen antigen 15-3, ⑧ glycogen antigen 19-9.

[0059] The surface of each chip detection card 13 is spray-printed with a one-dimensional code, containing product-related information, including product name, batch number, etc.

[0060] A total of 8 pieces of each chip detection card 13 are packed into one chip card storage box 12 .

[0061] A two-dimensional code is printed on the outer surface of the chip card storage box 12, which contains relevant information of the product, including: product name, batch number, standard curve, etc.

[0062] The detection method of the fully automatic microfluidic chip immune detection system includes the following steps.

[0063] (1) Fix the...

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Abstract

The invention belongs to the field of immune detection and analysis, and particularly relates to a full-automatic micro-fluidic chip fluorescence immune detection system and a detection method implemented by the same. The full-automatic micro-fluidic chip fluorescence immune detection system comprises a shell and a main framework, and is characterized in that a chip card storage disc and a sample tube disc are fixed to the upper portion of the main framework, a centrifugal reaction disc is arranged between the chip card storage disc and the sample tube disc, and a filling system and a cleaning system are arranged between the centrifugal reaction disc and the sample tube disc; an optical detection system is arranged on the main framework and corresponds to the centrifugal reaction disc, a control system and motors are arranged inside the main framework, and the chip card storage disc, the centrifugal reaction disc, the sample tube disc and the filling system can be driven by the motors to rotate. The full-automatic micro-fluidic chip fluorescence immune detection system and the detection method have the advantage that requirements on full automation, multiple samples, high flux and quick detection can be fundamentally met by the full-automatic micro-fluidic chip fluorescence immune detection system.

Description

technical field [0001] The invention belongs to the field of immune detection and analysis, and in particular relates to a fully automatic microfluidic chip fluorescent immune detection system and a detection method thereof. Background technique [0002] The detection system of immunoassay can be divided into many kinds according to different immunoassay methods, such as immunochromatography analyzer, enzyme-linked immunoassay analyzer, chemiluminescence immunoassay analyzer, etc. [0003] With the application of microfluidic chips (microfluidic chips) technology in medicine, life science and other fields, the microchannel structure and other functional components of the chip are integrated on the substrate of several square centimeters, and the fluid in the microchannel is controlled, It is a micro-full analysis system that realizes multiple functions such as sample injection, dilution, mixing, reaction, separation, and detection. It has significant advantages such as minia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53B01L3/00
CPCB01L3/5027B01L3/502707B01L3/502753B01L2200/0689B01L2200/10B01L2200/14B01L2200/16B01L2400/0409B01L2400/043G01N33/53
Inventor 李祝华白众瑜黄雪莹孙颖孙秒吕辉高占岩
Owner 沈阳优宁生物科技有限公司
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