Bilirubin electrochemical transducer and preparation method thereof

A bilirubin oxidase, electrochemical technology, applied in the field of electrochemical detection, can solve the problems of poor anti-interference ability, long reaction time, interference, etc., to achieve the effect of improving accuracy and strong anti-interference ability

Inactive Publication Date: 2015-07-22
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bilirubin oxidase method has a wide linear range, but the calculation formula of the bilirubin oxidase endpoint method is complicated, the reaction time is long, and it is easily interfered by serum background
[0007] The above three bilirubin detection methods are all photochemical methods, and all of them have the problem of poor anti-interference ability

Method used

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  • Bilirubin electrochemical transducer and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The preparation of embodiment 1 bilirubin electrochemical sensor

[0067] Conductive carbon paste was printed on PET using screen printing technology to make carbon electrodes.

[0068] Weigh 1g of copper sulfate, 0.01g of bilirubin oxidase, 0.5g of BYK163, 2g of carboxymethylcellulose, and 97g of pure water, and use a high-speed mixer to stir while adding, the speed is controlled at 600rpm, and the time is controlled at 15 minutes , after the enzyme solution is mixed, control the speed of the mixer at 1200rpm and stir for 3 hours to obtain the enzyme solution of the bilirubin oxidase system; use the screen printing technology to print the prepared enzyme solution on the carbon electrode, and dry it in an oven to form membrane to obtain a semi-finished bilirubin electrochemical sensor.

[0069] Weigh 0.2g of ascorbate oxidase, 0.5g of Tween-60, 2g of carboxymethyl cellulose, and 98g of pure water, and use a high-speed mixer to stir while adding, the speed is controlled...

Embodiment 2

[0073] The preparation of embodiment 2 bilirubin electrochemical sensor

[0074] Conductive carbon paste was printed on PET using screen printing technology to make carbon electrodes.

[0075] Weigh 1.5g of copper chloride, 15mg of bilirubin oxidase, 0.5g of TEGO Dispers 655, 2g of hydroxyethyl cellulose, 66g of pure water, and 30g of isopropanol, and stir while adding them using a high-speed mixer, with the speed controlled At 600rpm, the time is controlled at 15 minutes. After the enzyme solution is mixed, the mixer speed is controlled at 1200rpm and stirred for 3 hours to obtain the enzyme solution of the bilirubin oxidase system; the prepared enzyme solution is printed on carbon by screen printing technology. The electrodes are dried in an oven to form a film to obtain a semi-finished product of the bilirubin electrochemical sensor.

[0076] Weigh 0.1g of ascorbate oxidase, 0.5g of triton, 5g of hydroxyethyl cellulose, and 95g of pure water, and use a high-speed mixer to ...

Embodiment 3

[0078] The preparation of embodiment 3 bilirubin electrochemical sensor

[0079] Conductive carbon paste was printed on PET using screen printing technology to make carbon electrodes.

[0080] Weigh 1.5g of methylene blue, 15mg of bilirubin oxidase, 1g of EFKA4320, 2g of chitosan, 75g of pure water, 20g of ethanol, and use a high-speed mixer to stir while adding, the speed is controlled at 600rpm, and the time is controlled at After 15 minutes, wait for the enzyme solution to mix well, then control the speed of the mixer at 1200rpm and stir for 3 hours to obtain the enzyme solution of the bilirubin oxidase system; print the prepared enzyme solution on the carbon electrode using screen printing technology, and dry it in an oven. Dry into a film to obtain a semi-finished product of the bilirubin electrochemical sensor.

[0081] Weigh 0.3g of ascorbate oxidase, 1g of sodium lauryl sulfate, 1g of chitosan, and 98g of pure water, and use a high-speed mixer to stir while adding, th...

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PUM

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Abstract

The invention relates to the technical field of electrochemical detection, in particular to a bilirubin electrochemical transducer and a preparation method thereof. The bilirubin electrochemical transducer comprises an electrode, a bilirubin oxidase layer and an anti-interference enzyme layer, wherein the bilirubin oxidase layer is arranged between the electrode and the anti-interference enzyme layer, and comprises bilirubin oxidase and an oxidation-state electronic mediator; the anti-interference enzyme layer comprises ascorbic acid oxidase. The bilirubin electrochemical transducer has the advantages of being high in sensitivity, wide in linearity range, low in sample demand, high in anti-interference capacity, and short in detection time.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to a bilirubin electrochemical sensor and a preparation method thereof. Background technique [0002] Bilirubin is a kind of bile pigment, which is the main pigment in human bile and is orange-yellow. It is the main metabolite of iron porphyrin compounds in the body. It is toxic and can cause irreversible damage to the brain and nervous system, but it also has an antioxidant function, which can inhibit the oxidation of linoleic acid and phospholipids. Bilirubin is an important basis for judging jaundice clinically, and it is also an important indicator of liver function. For example: when the liver cells have lesions, or due to swelling of the liver cells (mostly caused by acute jaundice hepatitis, acute yellow liver necrosis, chronic active hepatitis, liver cirrhosis and other liver diseases), the bile ducts in the liver can be affected. pressure, bile excretion...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 尹辉军周建华
Owner SINOCARE
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