The invention discloses a sulphating
catathelasma ventricosum polysaccharide preparing method. The method includes the steps that
catathelasma ventricosum is frozen, dried and ground into
powder, extraction of
alcohol sedimentation is conducted through
ethanol water, deproteinization is conducted, small molecular weight
impurity removal is conducted, and the
catathelasma ventricosum polysaccharide is obtained; the method for preparing the sulphating catathelasma ventricosum
polysaccharide includes the steps that 70 ml of
dimethyl sulfoxide is weighed to be placed into a dry three-necked flask provided with a condensing unit, catathelasma ventricosum polysaccharide samples are added slowly to the flask while magnetic stirring is conducted, the samples are prevented from condensing into blocks, stirring is conducted for 1 hour, and a
bottle plug is arranged on the flask in a covered mode; then
sulfur trioxide-
pyridine powder is added, the mixture is heated to 85 DEG C in a water bath mode, a condenser
pipe conducts
backflow, and a reaction is conducted for 3 hours; after the reaction is completed, the flask is placed into a cold bath to be cooled to the
room temperature, then reaction liquid is neutralized through NaOH, absolute ethyl
alcohol is added for
sediment, the mixture stand still for a night, the
sediment is collected in a centrifugal mode and dissolved in water for
dialysis, the
dialysis liquid is frozen and dried, and the sulphating catathelasma ventricosum polysaccharide is obtained.