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Method for promoting wood dust enzymolysis through combined pretreatment

A technology of wood chips and enzymolysis, applied in fermentation and other directions, can solve the problems of poor pretreatment effect of wood chips and low enzymolysis yield, achieve co-production of xylo-oligosaccharides, improve the degree of hydrophilic modification, and strengthen the mitigation effect. Effect

Pending Publication Date: 2018-11-27
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: In order to solve the problems of poor pretreatment effect and low yield of enzymatic hydrolysis of existing sawdust, the present invention provides a method for promoting enzymatic hydrolysis of sawdust through combined pretreatment

Method used

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  • Method for promoting wood dust enzymolysis through combined pretreatment
  • Method for promoting wood dust enzymolysis through combined pretreatment
  • Method for promoting wood dust enzymolysis through combined pretreatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] An efficient combined pretreatment method for poplar wood chips, the steps are as follows:

[0020] (1) Weigh 200g of poplar wood chips with absolute dry weight, and treat them with 5% (v / v) acetic acid aqueous solution at a solid-to-liquid ratio of 1:10 (g / mL, the same below), at 170°C for 0.5h.

[0021] (2) After the acetic acid treatment finishes, carry out solid-liquid separation, the treatment liquid that produces is the acetic acid aqueous solution that is rich in xylo-oligosaccharide, and its xylo-oligosaccharide concentration and yield are shown in Table 1; The solid residue that it produces is washed with water, filtered Finally, poplar sawdust treated with acetic acid was obtained, and the analysis of its main components is shown in Table 1.

[0022] Wherein, xylo-oligosaccharide yield (%)=(oligosaccharide concentration in hydrolyzate g / L×10)÷(1000g×xylan content)×100%. In the formula: 1 is the total volume of acetic acid treatment, L; 1000 is the absolute dr...

Embodiment 2

[0037] An efficient combined pretreatment method for poplar wood chips, the steps are as follows:

[0038] (1) Weigh 200g of poplar wood chips with absolute dry weight, and treat them with 5% (v / v) acetic acid aqueous solution at a solid-to-liquid ratio of 1:10 (g / mL, the same below), at 170°C for 0.5h.

[0039] (2) After the acetic acid treatment is completed, solid-liquid separation is carried out, and the treatment liquid and solid residue are collected respectively; the solid residue is washed with water and filtered for later use.

[0040] (3) Take by weighing 20g of the above-mentioned acetic acid-treated poplar wood chips with a dry weight, use 30g / L sodium hydroxide aqueous solution, the amount of polyethylene glycol diglycidyl ether is 5-30%, and the solid-to-liquid ratio is 1:10g / mL, Co-processed at 70°C for 3h. Among them, the quality of polyethylene glycol diglycidyl ether is calculated by absolute dry weight of poplar wood chips treated with acetic acid.

[0041...

Embodiment 3

[0048] A high-efficiency combined pretreatment method for poplar wood chips, cellulase adsorption test, the specific steps are as follows:

[0049] (1) Weigh 200g of poplar wood chips with absolute dry weight, and treat them with 5% (v / v) acetic acid aqueous solution at a solid-to-liquid ratio of 1:10 (g / mL, the same below), at 170°C for 0.5h.

[0050] (2) After the acetic acid treatment is completed, solid-liquid separation is carried out, and the treatment liquid and solid residue are collected respectively; the solid residue is washed with water and filtered for later use.

[0051] (3) Take by weighing 20g of the above-mentioned acetic acid-treated poplar wood chips with a dry weight, use 30g / L sodium hydroxide aqueous solution, the amount of polyethylene glycol diglycidyl ether is 5-30%, and the solid-to-liquid ratio is 1:10g / mL, Co-processed at 70°C for 3h.

[0052] (4) After the combined treatment, the materials are collected by washing with water and filtered, and used...

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Abstract

The invention discloses a method for promoting wood dust enzymolysis through combined pretreatment. An acid treatment method and an alkali treatment method are combined for efficient combined pretreatment of poplar wood dust. The method includes: (1) preparing xylooligosaccharide from poplar wood dust through acid treatment; (2) subjecting acid treated poplar wood dust generated in the step (1) toalkali pretreatment, so that pretreated poplar wood dust cellulase hydrolysis yield is remarkably increased. In the step (2) of alkali treatment, polyethylene glycol diglycidyl ether is added for auxiliary treatment, cellulase nonspecific adsorption of lignin in an enzyme hydrolysis process is effectively weakened, and cellulase hydrolysis is promoted beneficially. According to experimental results, compared with existing research results, the method has the advantage that the enzyme hydrolysis yield is remarkably increased to 83.48%. In addition, by adoption of the method, xylooligosaccharide coproduction is realized, and a novel approach is provided for comprehensive utilization of poplar wood dust.

Description

technical field [0001] The invention relates to wood chip pretreatment and enzymolysis, in particular to a method for promoting wood chip enzymolysis through combined pretreatment. Background technique [0002] With the increasing demand and over-exploitation of fossil resources in human society, the fossil resources on the earth are inevitably facing the fate of exhaustion. Lignocellulosic raw material is the most abundant renewable resource on earth. It captures solar energy through photosynthesis of plants and stores it in the form of polymer. Using industrial biotechnology to convert lignocellulosic raw materials into alternative energy and chemicals is one of the important ways to alleviate the shortage of fossil resources. Lignocellulosic raw materials mainly include broad-leaved wood, coniferous wood, energy crops, crop straw, agricultural and forestry waste, and urban fiber waste. Poplar is an important economic tree species in my country. At present, it has formed...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14C12P2201/00
Inventor 赖晨欢勇强杨博王江龙黄曹兴李鑫陈牧徐勇欧阳嘉余世袁
Owner NANJING FORESTRY UNIV
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