The invention relates to a kiwi fruit extract and an extraction method and an application thereof. The extraction method of the kiwi fruit extract includes that fresh kiwi fruits with skins are cut up, the cut fresh kiwi fruits are heated by methanol and subjected to reflux extraction for two times, the first time of the reflux extraction is performed for 1.5 hours to 2.5 hours, the second time of the reflux extraction is performed for 0.5 hour to 1.5 hours, the twice extracting solutions are combined, filtered and concentrated to obtain a kiwi fruit methanol total extract, the kiwi fruit methanol total extract is subjected to extraction by petroleum ether, ethyl acetate and n-butyl alcohol sequentially, and after reduced pressure suction filtration and concentration, a kiwi fruit petroleum ether extracting part, an ethyl acetate extracting part and an n-butyl alcohol extracting part are respectively obtained. According to internal experiment, acarbose serves as positive control, the therapeutic effect of the kiwi fruit petroleum ether, the ethyl acetate and the n-butyl alcohol extracting parts on a diabetic mouse which is induced by alloxan is observed, and the experimental result shows that a certain therapeutic effect of kiwi fruit ACPE, ACEA and ACBU on the diabetic mouse which is induced by the alloxan is achieved.