37results about How to "Enhance immune stimulation" patented technology

The invention provides methods for modulating the immune response caused by CpG dinucleotide-containing compounds. The methods according to the invention enables both decreasing the immunostimulatory effect for antisense applications, as well as increasing the immunostimulatory effect for immunotherapy applications.

The invention discloses a vaccine adjuvant of swine mycoplasmal pneumonia live vaccine, which is mainly prepared from an ISCOM substrate, levamisole, traditional Chinese herbal polysaccharide and phosphate buffer. Each ml of adjuvant contains 0.1 to 2mg of Quil A in the ISCOM substrate, 2 to 10mg of levamisole, 20 to 100mg of traditional Chinese herbal polysaccharide and the balance of phosphate buffer. The combination of the ISCOM substrate, the levamisole and the traditional Chinese herbal polysaccharide can well make the best of respective advantages and complement each other, so that the adjuvant can strengthen the immune stimulating ability of the swine mycoplasmal pneumonia live vaccine and strengthen the immune effect of the vaccine, and has the advantages of simple and convenient preparation method, stable performance, convenient mass production and the like. When the vaccine adjuvant is applied, intramuscular injection immunization can be performed by blending a proper proportion of swine mycoplasmal pneumonia live vaccine and the adjuvant of the invention; the intramuscular injection immunization of the swine mycoplasmal pneumonia live vaccine is realized; and the defects of the traditional intrapulmonary injection immunization are overcome.

The invention discloses probiotic solid powder and a preparation method thereof. The probiotic solid powder comprises a mixture of active probiotics and active zeolite, wherein the active probiotic content is between 12 and 50 billion colony-forming units (CFU) / gram; and the active zeolite mixture comprises the following components in percentage by weight: 30 to 50 percent of zeolite, 20 to 40 percent of attapulgite clay and 10 to 30 percent of bentonite. The probiotic solid powder provides a high-quality and low-cost antibiotic substitute product for livestock breeding; and the probiotic solid powder with 1 to 4 million CFU of live bacteria is added into each gram of feed, so the growth speed of chickens, pigs and fish can be improved by 2.0 to 15.6 percent, the conversion rate of the feed can be improved by 1.1 to 9.3 percent, and the diarrhea rate and the death rate of animals can be reduced obviously. The preparation method of the probiotic solid powder is simple, has low cost andcan reduce environmental pollution; and the content of the obtained product bacteria is high.

The present invention provides an adjuvant-polymer construct comprising a polymer backbone which is covalently linked to 3 or more adjuvants, wherein the 3 or more adjuvants are each present in a pendant side chain, the adjuvants being connected to the polymer backbone either directly or via a spacer group.

Amphiphilic oligonucleotide conjugates that enhance adjuvant function are disclosed. The conjugates typically include: a lipophilic component, and conjugated thereto (directly or indirectly) an immunomodulating oligonucleotide that, if it were not conjugated to the lipophilic component, would suppress TLR7 and / or TLR8 stimulation. In the presence of albumin, these conjugates significantly enhance adjuvant function, in particular the function of TLR7 / 8-mediated adjuvants such as an imidazoquinolinamine. The conjugates can be administered, along with an adjuvant compound, to a subject in order to cause and / or enhance an immune response (for instance, to an infectious agent or a cancer antigen) in the subject.

The invention discloses a vaccine adjuvant of swine mycoplasmal pneumonia live vaccine, which is mainly prepared from an ISCOM substrate, levamisole, traditional Chinese herbal polysaccharide and phosphate buffer. Each ml of adjuvant contains 0.1 to 2mg of Quil A in the ISCOM substrate, 2 to 10mg of levamisole, 20 to 100mg of traditional Chinese herbal polysaccharide and the balance of phosphate buffer. The combination of the ISCOM substrate, the levamisole and the traditional Chinese herbal polysaccharide can well make the best of respective advantages and complement each other, so that the adjuvant can strengthen the immune stimulating ability of the swine mycoplasmal pneumonia live vaccine and strengthen the immune effect of the vaccine, and has the advantages of simple and convenient preparation method, stable performance, convenient mass production and the like. When the vaccine adjuvant is applied, intramuscular injection immunization can be performed by blending a proper proportion of swine mycoplasmal pneumonia live vaccine and the adjuvant of the invention; the intramuscular injection immunization of the swine mycoplasmal pneumonia live vaccine is realized; and the defects of the traditional intrapulmonary injection immunization are overcome.

The invention discloses a kit, method and application of a high-efficiency novel DC autovaccine, and belongs to the technical field of biology. The kit comprises a cytokine combination of GM-CSF, IL-4, FLT3-L and ATRA. According to the method, an existing GM-CSF+IL-4 cultivation technique is improved, is combined with FLT3-L to promote generation of pDC, mononuclear cells are increased to convert DC, generation of MDSC is inhibited by utilizing all-transretinoic acid (ATRA) to eliminate the negative adjustment effect of MDSC in preparation of DC vaccines, and an OK432 and CPH ODN co-stimulation mode is adopted to effectively promote mature of mDC and pDC and enhance the DC immunostimulation function.