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Probiotic solid powder and preparation method thereof

A probiotic and active probiotic technology, applied in the field of microorganisms, can solve the problems of complex production process, high equipment requirements, large equipment investment, etc., and achieve the effect of simplifying the separation and purification process, improving the number and quality of viable bacteria, and reducing production costs.

Active Publication Date: 2013-06-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the raw materials used in solid fermentation are by-products of agricultural product processing, which are rich in sources, low in price, low in investment, low in technical requirements, etc., but the products produced contain few viable bacteria and low purity
The production of probiotics by liquid fermentation has high efficiency, many types of biologically active substances, high viable count and high purity, but the production process is complex, equipment investment is large, and energy consumption is high. Especially the probiotics used in feed need to be made into solid powder. Vacuum freeze-drying or spray-drying technology is mainly used to make probiotics in the fermentation liquid into powder products, which requires high equipment requirements, large investment, and high production costs. In addition, some live bacteria will be inactivated during the high-temperature drying process, which will affect the content of the product. Effective number of viable bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 prepares zeolite mixture

[0054] (1) The ingredients of the zeolite mixture are mixed according to the following weight percentages: 40% of zeolite, 35% of attapulgite clay and 25% of bentonite are mixed and crushed, and the particle fineness after crushing is ≤2 mm; (2) the crushed zeolite The mixture is input into a roasting furnace for roasting, and the roasting temperature is controlled at 300-320°C; (3) The roasted zeolite mixture is input into a mill for grinding, and the particle size after grinding is ≤0.074 mm, and the package is active zeolite The mixture is set aside.

Embodiment 2

[0055] Embodiment 2 prepares bacillus fermented liquid

[0056] 2.1 Preparation of Bacillus subtilis fermentation broth

[0057] (1) Under aseptic conditions, place the preserved Bacillus subtilis strains in the selective isolation medium in the bacterial incubator, control the temperature of the bacterial incubator at 37-38°C, and cultivate at a constant temperature for 15-16 hours. After cooling, Put it in the refrigerator for the preservation of the production strain, and the temperature of the refrigerator should be controlled at 3-4°C;

[0058] The ingredients of the selective separation medium are composed of the following components by weight percentage: 2.4% agar, 1.3% peptone, 0.6% glucose, 0.4% beef extract, 0.4% yeast extract, 0.3% sodium chloride and 95% water;

[0059] The ingredients for producing strains are composed of the following components in percentage by weight: 6% of strains of Bacillus subtilis and 94% of selective isolation medium.

[0060] (2) Under...

Embodiment 3

[0080] The preparation of embodiment 3 bacillus solid powder and bacterium content determination process

[0081] 3.1 Preparation process of Bacillus subtilis solid powder

[0082] The ingredients of Bacillus subtilis solid powder are composed of the following components by weight percentage: 70% of Bacillus subtilis fermentation liquid and 30% of active zeolite mixture. At 150-180 rpm, the temperature is controlled at 35-45°C, and the adsorption time is controlled at 45-50 minutes. After standing for 15 minutes, filter and separate with a filter cloth. The separated solid material is dried at 55-65°C. The dried solid material is pulverized, and the particle size of the pulverized powdery material is ≤0.1 mm, and the powdery material is packaged as a finished Bacillus subtilis solid powder.

[0083] 3.2 The preparation process of bacillus licheniformis solid powder

[0084] The ingredients of Bacillus licheniformis solid powder are composed of the following components by wei...

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Abstract

The invention discloses probiotic solid powder and a preparation method thereof. The probiotic solid powder comprises a mixture of active probiotics and active zeolite, wherein the active probiotic content is between 12 and 50 billion colony-forming units (CFU) / gram; and the active zeolite mixture comprises the following components in percentage by weight: 30 to 50 percent of zeolite, 20 to 40 percent of attapulgite clay and 10 to 30 percent of bentonite. The probiotic solid powder provides a high-quality and low-cost antibiotic substitute product for livestock breeding; and the probiotic solid powder with 1 to 4 million CFU of live bacteria is added into each gram of feed, so the growth speed of chickens, pigs and fish can be improved by 2.0 to 15.6 percent, the conversion rate of the feed can be improved by 1.1 to 9.3 percent, and the diarrhea rate and the death rate of animals can be reduced obviously. The preparation method of the probiotic solid powder is simple, has low cost andcan reduce environmental pollution; and the content of the obtained product bacteria is high.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a probiotic solid powder and a preparation method thereof. Background technique [0002] Probiotics play a role by improving the balance of microorganisms in the intestinal tract. They are beneficial living microorganisms that can be directly fed to animals. Probiotics are safe, environmentally friendly, promote animal growth, inhibit the reproduction of pathogenic bacteria, prevent digestive tract diseases, and enhance animal immunity. Function, improve feed utilization, supplement nutrition, improve water environment, remove toxins and other effects. [0003] At present, the probiotics used in feed are all solid powder products. The production process of probiotics mainly includes solid fermentation method and liquid fermentation method. Most of the raw materials used in solid fermentation are by-products of agricultural product processing, which are rich in sources,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/175A23K10/18A23K20/28
Inventor 周岩民孙亚楠王龙昌王恬
Owner NANJING AGRICULTURAL UNIVERSITY
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