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85results about How to "Enable early detection" patented technology

Air-gap field detecting device of alternating-current generator and application thereof in state monitoring and control method of alternating-current generator

The invention relates to an air-gap field detecting device of an alternating-current generator, comprising a detecting coil and a signal detecting and processing circuit, wherein the detecting coil is arranged on a rotor of the alternating-current generator and surrounds one zone on the inner surface of the stator, and the induced voltage of the detection coil is relevant to the strength of an air-gap field in the zone surrounded by the detecting coil, as well as the distribution condition and the changes of the air-gap field; the signal detecting and processing circuit is connected with the detecting coil and used for detecting and processing the induced voltage of the detecting coil; the signal detecting and processing circuit can be used for detecting and processing the fundamental waves or the harmonic waves of the induced voltage of the detecting coil, or detecting and comparing the induced voltages of different detecting coils, and the detection and processing result can be used for the monitoring of the states of the generator, including the faults of stator and rotor windings, the decentraction of a stator and a rotor, axial and opposite shift of the stator and the rotor, magnetism loss, overload, external short circuit and circuit breakage and the like; and the detection and processing result of signals can also be used for the operation control of the generator.
Owner:田京涛

Papillomavirus detection and parting method as well as liquid phase chip thereof

The invention discloses papillomavirus detection and a typing liquid chip, and a process which is used to the detection and typing papillomavirus. The liquid chip mainly comprises microsphere which is respectively enveloped with specific anti-tag label sequence and 7-10 T spacer arm sequence which is arranged between anti-tag label sequence and microsphere, wherein anti-tag label sequence is chosen from two or more than two sequences in SEQ ID NO. 25- SEQ ID NO.47, specific ASPE primer is respectively designed aiming at each typing HPV, ASPE primer is chosen from two or more than two sequence from SEQ ID NO.1-SEQ ID NO.23, and primer of target sequence which has mutant sites is amplified. The liquid chip improves the current liquid chip technology, which makes ribonucleotide probe microspheres which is prepared be suitable to different detection projects, largely increases fluorescent signal value which is detected, thereby further increasing sensitivity of the detection, strengthening signal-to-noise ratio, and making detection results more accurate and reliable.
Owner:SUREXAM BIO TECH

Sea urchin-shaped hollow gold and silver alloy nano particle and preparation method and application thereof

The invention discloses a sea urchin-shaped hollow gold and silver alloy nano particle and a preparation method and application thereof. The method comprises the following steps: dissolving silver nitrate into water; heating the mixture of the silver nitrate and the water till boiling; adding trisodium citrate aqueous solution in the mixture and keeping boiling for 3-30 minutes to obtain a silver colloid; mixing chloroauric acid aqueous solution and the water; adding the silver colloid under the condition of agitation at the temperature of 15 DEG C; adding hemoporphyrin metalporphyrin aqueous solution and obtaining a reaction solution after 5 min reaction; performing centrifugalization of the reaction solution, washing deposit obtained via centrifugalization, and obtaining the sea urchin-shaped hollow gold and silver alloy nano particle. The diameter of the nano particle is 80 nm-300 nm, the hollow size is 20 nm-50 nm, the length of a stab is 10 nm-30 nm, the diameter of the stab is 10 nm. The preparation method is simple, low in cost, gentle in condition, short in time, easy in process control, and large in production capacity. The obtained nano particle can be well applied to organic molecule detection, earlier detection of tumour and photothermal treatment.
Owner:XI AN JIAOTONG UNIV

Indirect distributed denial of service attack defense method and system based on Web agency

InactiveCN102438025AAttack Response ImplementationEnable early detectionData switching networksSmall probabilityHidden semi-Markov model
The invention relates to an indirect distributed denial of service attack defense method and an indirect distributed denial of service attack defense system based on a Web agency. A behavior characteristic of a proxy-to-server network flow is described by extracting the space-time local property of the proxy-to-server network flow; the interference of a small-probability large value on an available signal is restrained by a nonlinear mapping function; a normal behavior model of the proxy-to-server network is constructed through a hidden semi-markov model (HsMM); normal degree estimation, namely long-time behavior estimation and short-time behavior estimation, under different time scales is performed by using behavior indexes acquired by the model; as to an abnormal behavior sequence (HTTP request sequence), an attack response is implemented by adopting a soft control method; and the basis of the soft control represents an HsMM model parameter and a structure index which are used for performing a normal behavior. The parameter for describing the proxy-to-server network is the space-time local property which is irrelevant to the change of the Web content on a target server; and the detection property of the method is the nature property based on the agent network flow and irrelevant to the size of the attack flow. By the method, the attack response can be realized before the resources of the target server are used by the attack flow, so that early detection can be realized effectively.
Owner:SUN YAT SEN UNIV

Disaster relieving system and method

The invention provides disaster relieving system and method. The disaster relieving system comprises a detecting subsystem and a disaster relieving subsystem; the detecting subsystem comprises a firstacquisition module used for acquiring disaster monitoring information in a protection zone and a disaster judgment module connected with the first acquisition module and used for judging whether a disaster happens or not according to the disaster monitoring information; and the disaster relieving subsystem comprises a disaster relieving execution module connected with the disaster judgment moduleand used for executing disaster relief when the disaster happens. The invention integrates early detection of disasters and automatic disaster relief into a whole, thereby improving the disaster preventing and relieving capacity of the disaster relieving system.
Owner:北京英特威视科技有限公司

Kit for detecting colorectal cancer and precancerous lesions and use method thereof

PendingCN109097471AReduce the rate of missed diagnosis and missed detectionSampling is convenientMicrobiological testing/measurementBiological testingKRAS Gene MutationSulfite
The invention relates to a kit for detecting colorectal cancer and precancerous lesions and a use method thereof. The invention provides the kit for detecting colorectal cancer and precancerous lesions, and the kit comprises a nucleic acid separation and purification reagent, a DNA sulfite conversion reagent, a KRAS gene mutation detection reagent, an SDC2 and SFRP2 gene methylation detection reagent and a fecal occult blood detection reagent, wherein the nucleic acid separation and purification reagent is used for separating and purifying human DNA in a fecal sample; and the DNA sulfite conversion reagent is used for converting the partial purified human DNA into sulfite for subsequent detection of the methylation level of SDC2 and SFRP2 genes. The sensitivity of the kit for detecting thecolorectal cancer can reach up to 90% or more and the specificity is about 85%, thereby realizing high-sensitivity and high-specificity detection of the colorectal cancer; and the kit provided by theinvention can detect most precancerous lesions of the colorectal cancer to realize ultra-early detection of the colorectal cancer, and can greatly reduce missed diagnosis and missed detection rates of clinical existing methods when being used for assisting clinical diagnosis.
Owner:HANGZHOU HEYI GENE TECH

Early fire detection method, computer readable medium and system

The invention relates to an early fire detection method which is used for detecting a fire and comprises the following steps: S1, acquiring a real-time monitoring video; S2, providing a single-frame detection network model, and using the single-frame detection network model to detect whether flame and / or smoke exists in the image at the current moment or not so as to obtain a first detection result; S3, providing a time sequence detection network model, and detecting a flame level and / or a smoke level in the video sequence before the current moment by using the time sequence detection networkmodel to obtain a second detection result; and S4, calculating a fire risk level by combining the first detection result and the second detection result. Thus, missed detection of fire disasters is prevented, and the accuracy of fire detection is improved. The invention also provides a computer readable medium. The invention also provides a detection system.
Owner:创新奇智(成都)科技有限公司

Detection method of sweet potato black rot pathogen

ActiveCN103667494APrevent the spread of the spreadPrevention and treatment of misdiagnosisMicrobiological testing/measurementMicroorganism based processesPathogenMycelium
The invention relates to a detection method of sweet potato black rot pathogen, and belongs to the technical field of bioengineering. The method specifically comprises steps as follows: a pair of sweet potato black rot pathogen specific primers SPCPF and SPCPR is designed and synthesized; total DNA (deoxyribonucleic acid) of a to-be-tested sample is extracted and taken as a template, and the primers SPCPF and SPCPR are used for performing PCR (polymerase chain reaction) amplification; and an amplification product is detected through agarose gel electrophoresis. The method is simple, convenient, fast and sensitive, not only can be used for detecting pathogen mycelia but also can be used for detecting sick potato seedlings and pieces; the detection method can be used for performing early detection on sweet potato black rot and further can be used for effectively distinguishing similar diseases, such as a black nevus disease, soft rot and dry rot, of sweet potatoes; and the method has significance on early warning and propagation prevention and control of the sweet potato black rot pathogen.
Owner:INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI

Phomopsis asparagi LAMP (loop-mediated isothermal amplification) detection primer pair and detection method thereof

The invention provides a phomopsis asparagi LAMP (loop-mediated isothermal amplification) detection primer pair and a detection method thereof, which are specially used for specific detection of phomopsis asparagi, and belongs to the field of disease detection and biological technologies for crops. The phomopsis asparagi LAMP detection primer pair comprises outer primers F3 and B3 and inner primers FIP and BIP and has the sequence in SEQ ID NO.1-4. According to the phomopsis asparagi detection method established on the basis of the primer pair, green fluorescence or ladder bands with the LAMPfeatures can be observed through a chromogenic reaction or agarose gel electrophoresis detection after LAMP constant-temperature amplification. According to the LAMP detection primer pair and the detection method thereof, rapid, sensitive and accurate detection of plants infected with phomopsis asparagi can be realized in production practice, besides, the LAMP detection primer pair and the detection method can be used for early diagnosis of field diseases and monitoring and identification of germs and reliable technological and theoretical bases are provided for early warning and control of the phomopsis asparagi.
Owner:INST OF PLANT PROTECTION FAAS

Broad-leaved wood log internal quality assessment system and application thereof

The invention discloses a broad-leaved wood log internal quality assessment system, which obtains response signals by mainly performing an acoustic impact test on the log, and then analyzes the response signal to evaluate the health status inside the log. The specific scheme is that: using the acoustic impact test as a non-destructive assessment method to test defects such as decay and voids in the log; obtaining response signals through the acoustic impact test on the log; and then analyzing the response signals to assess the health status inside the log; which meets actual use requirements.
Owner:NANJING FORESTRY UNIV

ELISPOT detection kit for detecting bovine brucellosis

The invention provides an ELISPOT detection kit for detecting bovine brucellosis.The ELISPOT detection kit comprises a supporting medium, a capture antibody, a detection antibody, a negative control, a positive control and a specificity irritant, wherein the capture antibody is the first bovine gamma interferon monoclonal antibody, the detection antibody is the second marked bovine gamma interferon monoclonal antibody, and the specificity irritant is brucellin Br-PPD.A micropore filter membrane is arranged on a contact face of the supporting medium and the reagent.The capture antibody coats the supporting medium or the capture antibody and the supporting medium are placed respectively.The capture antibody and the detection antibody can be subjected to the antigen antibody combination effect together with bovine gamma interferon.According to the detection kit, the adopted monoclonal antibody excreted by hybrid tumor cell strains has the advantages of being high in active valence, good in specificity and strong in natural antigen affinity.Compared with a brucellosis antibody ELISA detection kit, the ELISPOT can achieve early detection of bovine brucellosis infection, false positive interference brought by a cross reaction is eliminated, and the good specificity is shown.
Owner:YANGZHOU UNIV

Detection reagent strip utilizing recombinant fluorescent phycobiliprotein subunit, and preparation method thereof

A detection reagent strip utilizing a recombinant fluorescent phycobiliprotein subunit comprises a non-fluorescence bottom plate, first non-fluorescence filter paper, a detection protein binding membrane, a fluorescent sample pad and second non-fluorescence filter paper, wherein a detection line and a control line are arranged on the detection protein binding membrane; a detection antibody is marked on the detection line; a control antibody is marked on the control line; the fluorescent sample pad comprises a glass fiber membrane and a mixture of a streptavidin blended phycobiliprotein subunit and a biotinylated antibody; the mixture covers the glass fiber membrane. In addition, the invention further provides a preparation method of the detection reagent strip utilizing the recombinant fluorescent phycobiliprotein subunit. The detection reagent strip utilizing the recombinant fluorescent phycobiliprotein subunit can perform qualitative and quantitative detection on antigens or antibodies of diseases such as tumor and microbial infection, and the recombinant fluorescent phycobiliprotein subunit used by the reagent strip is environmentally friendly. The preparation method is low in purification preparation cost and environmentally friendly.
Owner:YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI

Three kits for detecting human phosphorylated Tau protein and preparation method thereof

The invention belongs to the field of immunodetection, and particularly discloses three kits for detecting human phosphorylated Tau protein and a preparation method thereof. According to the three detection kits disclosed by the invention, the monoclonal antibody 3G5 is used as a capture antibody, and the monoclonal antibody 4B1 is used as a detection antibody, or the monoclonal antibody 4B1 is used as a capture antibody, and the monoclonal antibody 3G5 is used as a detection antibody, wherein p-hTau396, 404 protein can be detected by the kit. The three detection kits constructed by using the monoclonal antibodies 3G5 and 4B1 are respectively suitable for detection in different scenes. The method can rapidly and accurately detect the human phosphorylated Tau, integrates the characteristics of specificity of the antibody, high sensitivity of ELISA, efficient enrichment of the immune nano magnetic beads, convenience and rapidness of the colloidal gold test strip and the like, and has the characteristics of accuracy, rapidness, high efficiency, specificity and sensitivity. Therefore, the method is suitable for rapid and sensitive early diagnosis of Alzheimer's disease, and is of great significance to timely and accurate guidance of medicine use for patients.
Owner:HUAZHONG UNIV OF SCI & TECH

Method for detecting trichinella circulating antigen by utilizing IgY-McAb sandwich ELISA (enzyme-linked immuno sorbent assay)

The invention discloses a method for detecting a trichinella circulating antigen by utilizing IgY-McAb sandwich ELISA (enzyme-linked immnuo sorbent assay). With an anti-trichinella muscle larval ES antigen egg yolk antibody IgY as a capture antibody and an anti-trichinella ES antigen McAb as a detection antibody, the preparation method of the IgY comprises the following steps of: adding the trichinella muscle larval into a culture medium to carry out sterile culture, purifying and dialyzing supernate and then concentrating and drying to obtain the ES antiagen, determining protein concentration and then immunizing roman legehenne by utilizing the ES antigen, collecting the IgY from the produced egg yolk by adopting a saturate ammonium sulphate precipitation method, purifying and determining concentration, and sub-packaging for later use; and the preparation method of the McAb comprises the following steps of: establishing a hybridoma cell strain secreting the anti-trichinella muscle larval ES antigen, cloning by adopting a limiting dilution method, purifying McAb by applying an octanoic acid-ammonia sulphate method, determining the concentration and sub-packaging for later use. The invention has the advantage that a new method which has high sensitivity and specificity and has wide application prospect is provided for early detection of trichinella CAg in blood serum of an infected animal.
Owner:ZHENGZHOU UNIV

Hybridoma cell strain secreting anti-serine phosphorylated tau protein monoclonal antibody

The invention belongs to the field of immunodetection, and discloses two hybridoma cell strains secreting anti-serine phosphorylation tau protein monoclonal antibodies, and the hybridoma cell strains used for secreting p-Tau 396, 404 monoclonal antibodies, the hybridoma cell strains are preserved in the China Center for Type Culture Collection (CCTCC), and the preservation numbers are respectively CCTCC NO: C2020219 and CCTCC NO: C2020218. According to the invention, two hybridoma cell strains capable of secreting novel p-Tau 396, 404 monoclonal antibodies are provided, and the monoclonal antibodies capable of specifically recognizing human p-Tau 396, 404 and aggregates thereof can be prepared by using the hybridoma cell strains, so that the problem of lack of antibodies capable of simultaneously targeting p-Tau 396, 404 at present is solved. The two monoclonal antibodies provided by the invention can be further applied subsequently to prepare a high-specificity and high-sensitivity p-Tau 396, 404 detection kit, the immunological treatment of specific targeting p-Tau 396, 404 is developed, the disease progress is slowed down, and the blank of the current immunological treatment of targeting p-Tau 396, 404 is filled up.
Owner:HUAZHONG UNIV OF SCI & TECH
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