The invention provides an upward and downward staged rock drilling, sublevel bottom cutting and ore removal subsequent backfilling mining method. The method includes the following steps that panels are divided in the ore direction, a stope preparation system is arranged, upward fan-shaped medium-depth blast holes are drilled from one side to the other side of a chamber, the blast holes are filled with explosives for detonation, and an undercut space is formed after detonation; cut grooves are formed through a VCR method, and as for ore above the undercut space, downward vertical deep holes are drilled in a drilling chamber at the top of the chamber, and the holes are filled with explosives for detonation with the cut grooves as a compensation space; the chamber in each panel is subjected to two-step backstoping, after ore removal of a first-step chamber is finished, the first-step chamber is filled with cementing materials, after the first-step chamber is filled and cured, a second-step chamber is subjected to backstoping, the backstoping steps of the second-step chamber are the same as those of the first-step chamber, and the second-step chamber is filled with cemented filling bodies and non-cemented filling bodies; and the steps are repeated in this way till backstoping of all chambers are completed. The upward and downward staged rock drilling, sublevel bottom cutting and ore removal subsequent backfilling mining method has the advantages that the rock drilling efficiency of the upward fan-shaped medium-depth blast holes is improved, the waste hole rate is lowered, efficient and safe bottom ore removal is achieved, and the mechanization level is high.