The invention discloses a disposable isolation ward and a use method thereof, and belongs to the technical field of medical supplies. The ward comprises an HEPA filter, filter control keys are arranged at the top of the front wall of the HEPA filter, a wall storage groove is formed in the right side of the top of the HEPA filter, the left side of the top of the HEPA filter is connected with a first longitudinal adjusting rod through a screw, second longitudinal adjusting rods are arranged on the right side, the rear side and the diagonal side of the first longitudinal adjusting rod, and transverse folding rods are connected between the first longitudinal adjusting rod and the second longitudinal adjusting rod on the right side and between the first longitudinal adjusting rod and the secondlongitudinal adjusting rod on the rear side. According to the disposable isolation ward, the lightness and convenience are achieved, the firmness is achieved, the mobility is high, the construction is convenient, the relevant cost of terminal disinfection can be reduced, the rapid check-in of patients is facilitated, PPE walls can be conveniently stored, the patients with low immunity are protected, transmission of infectious viruses is controlled, the infectious patients are isolated, the disinfection effect on object surfaces, air and the ground is achieved, and the isolation ward construction cost of medical institutions is reduced.