The invention provides a groove type cell tray detection device. The groove type cell tray detection device comprises a foundation, a detection simulation block, an overturning simulation block, a clamping block and detection pins, wherein the foundation is provided with the detection simulation block, the detection simulation block is provided with a detection groove in consistent with detected cell trays in shape and can provide multi-directional detection for products, the detection groove is provided with multiple circular holes and elliptical holes, the circular holes and elliptical holes are respectively embedded with metal nuts internally through injection molding, service life is prolonged, one end of the overturning simulation block is in movable connection with a positioning column, the other end is fixed with the clamping block, and the groove type cell tray detection device is used for detecting slot structures of products. The groove type cell tray detection device is advantaged in that the detection simulation block employs groove design, full-directional detection on the cell trays can be realized, detection areas are large, unified standards are not only provided for product detection, moreover, detection results are more visual, persuasion is more powerful, and operation is simple.