A kind of detection method of citrulline content in citrulline raw material
A detection method and citrulline technology, applied in the field of biological analysis, can solve the problems affecting the accuracy of quantitative analysis of citrulline, damage to the service life of chromatographic columns, and expensive derivatization reagents, and achieve more convincing and shortened detection results. The effect of detecting time and reducing the error rate of experimental operation
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Embodiment 1
[0061] The equipment, reagents and detection conditions used in this embodiment are as follows:
[0062] High performance liquid chromatography: Thermo Fisher Dionex Ultimate 3000; VWD UV detector;
[0063] Chromatographic column: Thermo Fisher Syncronis C18 (5μm, 4.6×250mm);
[0064] Column temperature: 30°C;
[0065] Wavelength: 190nm;
[0066] Flow rate: 0.6mL / min;
[0067] Injection volume: 10μL;
[0068] Mobile phase: Mobile phase A is a mixed solution of 0.05mol / L dipotassium hydrogen phosphate and 0.005mol / L sodium decanesulfonate, the pH value is 2.3, and mobile phase B is absolute ethanol;
[0069] Mobile phase volume ratio: 1000:70.
[0070] The specific operation steps for the determination of the content of citrulline in the reference substance:
[0071] (1) Reference substance solution preparation
[0072] Citrulline control stock solution: Accurately weigh an appropriate amount of citrulline reference substance, and dilute with deionized water to obtain a ...
Embodiment 2
[0076] The equipment, reagents and detection conditions used in this embodiment are as follows:
[0077] High performance liquid chromatography: Thermo Fisher Dionex Ultimate 3000; VWD UV detector;
[0078] Chromatographic column: Thermo Fisher Syncronis C18 (5μm, 4.6×250mm);
[0079] Column temperature: 30°C;
[0080] Wavelength: 190nm;
[0081] Flow rate: 0.6mL / min;
[0082] Injection volume: 10μL;
[0083] Mobile phase: Mobile phase A is a mixed solution of 0.05mol / L dipotassium hydrogen phosphate and 0.005mol / L sodium decanesulfonate, the pH value is 2.3, and mobile phase B is absolute ethanol;
[0084] Mobile phase volume ratio: 1000:70.
[0085] Determination of citrulline content in raw materials Concrete steps:
[0086] (1) Take 0.1g of citrulline raw material (the purity of citrulline is known to be 98.11%) and place it in a 100mL volumetric flask, use deionized water to set the volume to the mark, and shake well to obtain citrulline with a concentration of 1g / L...
Embodiment 3
[0090] The equipment, reagents and detection conditions used in this embodiment are as follows:
[0091] High performance liquid chromatography: Thermo Fisher Dionex Ultimate 3000; VWD UV detector;
[0092] Chromatographic column: Thermo Fisher Syncronis C18 (5μm, 4.6×250mm);
[0093] Column temperature: 30°C;
[0094] Wavelength: 190nm;
[0095] Flow rate: 0.6mL / min;
[0096] Injection volume: 10μL;
[0097] Mobile phase: Mobile phase A is a mixed solution of 0.05mol / L dipotassium hydrogen phosphate and 0.005mol / L sodium decanesulfonate, the pH value is 2.3, and mobile phase B is absolute ethanol;
[0098] Mobile phase volume ratio: 1000:70.
[0099] Citrulline linearity test specific steps:
[0100] (1) Accurately draw an appropriate amount of citrulline contrast stock solution prepared in Example 1 for 10 times respectively, place them in five 10ml volumetric flasks respectively, and dilute with deionized water to obtain a concentration of 0.10g / L, 0.20g / L, 0.41g / L, ...
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