The invention relates to a method for simultaneously detecting 7 conjugated estrogens in
livestock and poultry excrements through combination of
microwave extraction-
solid phase extraction pretreatment and liquid
chromatography-
mass spectrometry technology. The method comprises the following steps: 1) carrying out pretreatment on an excrement sample, adding two
internal standard substances indicating
recovery rate, that is, 17beta-estradiolD3 3-beta-D-
glucuronide and 17 beta-estridiol
sodium sulfate-D4, then, carrying out extraction
solvent microwave extraction and extraction solution filtering and collecting, and diluting the solution with water to adjust the pH value; 2)carrying out
solid phase extraction enrichment and purification on target estrogens; and 3) through an
internal standard method, quantitatively detecting 7 conjugated estrogens in the
livestock and poultry excrements through a liquid
chromatography-
mass spectrometer. By optimizing the extraction method, enrichment andpurification conditions, and liquid
mass testing conditions, and meanwhile, by selecting appropriate
internal standard substances, the method can simultaneously detect residue information of the 7 conjugated estrogens in the
livestock and poultry excrements at one time; and the method has the advantages of high
recovery rate, high sensitivity, high stability, low
detection limit and more realistic and reliable detection results and the like.