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Dual-gated related fluorescence imaging device and imaging method

A fluorescent imaging and imaging method technology, applied in the field of fluorescent markers and optical components in systems or instruments, can solve the problems of fluorescent light intensity, difficulty in distinguishing target objects, difficulty in extracting target interpretation content, etc., to enhance contrast and solve interference Effect

Active Publication Date: 2019-02-01
INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, fluorescence time-resolved imaging is to open the gate at a certain time delay to obtain fluorescence images under nanosecond or even picosecond exposure time at a certain moment. Difficult to extract target interpretation content
In addition, in the trace imaging inspection of fluorescent substances, because the content of fluorescent substances is low, the fluorescence intensity is very weak, and it is difficult to distinguish the target through the fluorescence time-resolved intensity image only by naked eyes

Method used

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  • Dual-gated related fluorescence imaging device and imaging method
  • Dual-gated related fluorescence imaging device and imaging method
  • Dual-gated related fluorescence imaging device and imaging method

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Embodiment

[0049] see figure 1 As shown, an embodiment of the present invention provides a dual-gated correlation fluorescence imaging device, including:

[0050] A laser 1 emitting pulsed laser light;

[0051] Further, the laser is a nanosecond or picosecond laser, and the pulse width is on the order of picoseconds.

[0052] In some embodiments, the laser 1 is a narrow nanosecond or picosecond laser with a pulse width on the order of picoseconds, and a laser probe with a wavelength of 266nm-532nm is selected for the laser. According to the target material (paper and traces), different laser wavelengths are selected for matching.

[0053] A shaping lens 2, used for beam expansion and shaping of the pulsed laser;

[0054] In some embodiments, the shaping lens 2 expands and shapes the light spot emitted by the laser 1 , and makes the light spot irradiate one or more characters according to the size of the characters on the detected paper.

[0055] A sample stage 3, used to place the sa...

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Abstract

The invention discloses a dual-gated related fluorescence imaging device and an imaging method. The device comprises a narrow pulse nanosecond or a picosecond laser, a shaping lens, a sample table, anultraviolet cut-off light filter, a nanosecond order gating image enhancement charge coupling equipment, a computer, and a timing control unit. The imaging method disclosed by the invention realizingthe dynamic time scanning through the hundreds of picoseconds grade online encoding timing control technology, so that acquiring two fluorescence time resolution images at different delays by utilizing the fluorescence lifetime difference between the target and the background; enhancing the contrast of the target fluorescence image and achieving the extraction purpose of the target content by inverting the fluorescence relative service life image by using an image enhancement related algorithm, and fusing the obtained fluorescence relative life diagram with the fluorescence time resolution image to obtain an enhanced color RGB fluorescence relative service life diagram, and solving the problem of target-background fluorescence interference.

Description

technical field [0001] The invention belongs to the field of fluorescent markers on optical components, systems or instruments, and in particular relates to a double-gated correlation fluorescence imaging device and an imaging method. Background technique [0002] Interference between target fluorescence and background fluorescence is a difficult problem in trace imaging inspection of fluorescent substances. In trace inspection, the target and the background are relative. For example, in document inspection, fluorescent substances can be relative targets to paper and other carriers. However, because the paper contains fluorescent agents, the fluorescence intensity produced by it will interfere with the imaging of fluorescent substances. Traditional fluorescence imaging inspection technology mainly measures the fluorescence intensity, which is easily affected by excitation light intensity, fluorophore concentration, photobleaching, etc., which is not conducive to the detectio...

Claims

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Application Information

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IPC IPC(8): G01N21/01G01N21/64
CPCG01N21/01G01N21/6402
Inventor 钟鑫王新伟孙亮董晗周燕
Owner INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
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