41 results about "Sudan Red G" patented technology

The invention discloses a method for simultaneous determination of a plurality of synthetic pigments in hot pot flavorings. The method comprises extraction, concentration, purification and detection of new red, amaranth, carmine, allura red, erythrosine, sunset yellow, acid red G, acid scarlet GR, Rhodamine B, Para Red, sudan I, sudan II, sudan III, sudan IV, sudan orange G, sudan red 7B and a plurality of other synthetic pigments. The method for simultaneous determination of a plurality of synthetic pigments in hot pot flavorings provided by the invention has the advantages of simple steps and high accuracy and precision.

The invention discloses a Sudan I immune chromatography test paper detection method. On the back liner of the test paper, a sample liquor absorption part, a colloidal gold marking part, a detecting reaction part and a water absorption part are sequentially pasted, wherein, the detecting reaction part is covered with one or two strips of Sudan I polyclonal antibodies or one or two strips of antigens used for detecting, which are used as detecting lines, meanwhile, one strip of IgG which resists the second species animal protein also covers the detecting reaction part and is used as a reference line. The combination mode has two ways, that is, one detecting line plus one reference line or two detecting lines plus one reference line. The fast detection test paper can be used when the temperature ranges from 4 DEG C to 40 DEG C and is suitable for fast detecting foods such as meat, etc. which may be polluted by Sudan I by departments such as sanitary, quality supervision, customs and supermarkets, etc. The Sudan I immune chromatography test paper detection method has the advantages of strong specificity, high sensitivity, capability for realizing semi-quantitative detection, simple and convenient operation, etc.

The invention discloses a composite adsorbing material of a metal organic framework and cuprous salt and a preparation method and application thereof. The adsorbing material is compounded by the metal organic framework HKUST-1 and cuprous salt and is a brand-new composite adsorbing material, wherein the HKUST-1 has a very high specific surface area and can form physical adsorption with azobenzene dyes such as methyl orange, and the cuprous salt is attached to the surface of the KHUST-1 and is uniformly dispersed, so that the adsorption capacity of the material on organic dyes is favorably improved. The preparation method disclosed by the invention is simple and feasible and is easy to realize; and furthermore, the composite material can be widely applied to organic dyes for removing azobenzene, and particularly has good application prospects in the aspect of removing dyes, such as methyl orange, Sudan red, reactive black, methyl red and dimethyl yellow.

The invention discloses a the fat-soluble azo dye detection method and kit in the food or feed, which includes the following steps: (1) Preparation for the test solution: to be examined samples using organic solvent dilution, dissolved or extraction, as evidence test solution; (2) adding the test solution of excessive oxidant; (3) adding reducing agent to remove excess oxidizer, (4) static hierarchical, according to the color of organic layer judge whether the seized samples containing Sudan, and other fat-soluble azo dyes. The detection method of this invention is low cost, specific, sensitive and simple rapid, and does not require expensive equipment, and it can be used for the scene rapid detection of food or feed whether added illegal Sudan, and other fat-soluble azo dyes. At the same time it provides an apid screening test to see whether the food or feed contain Sudan or fat-soluble azo dyes illegally.

The invention discloses a method for simultaneously determining seven pigments in eggs, and belongs to the technical field of food safety detection. According to the method, a DQTP / Fe3O4 organic covalent skeleton structure and a magnetic nano composite material are adopted as a distributed magnetic solid-phase extraction adsorbent to extract pigments in eggs, and then an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) method is combined with an external standard method to determine the content of the pigments in the eggs. The invention discloses a method forsimultaneously determining contents of riboflavin, xanthophyll, canthaxanthin, Sudan red I, Sudan red II, Sudan red III and Sudan red IV pigments in eggs. Detection limits of xanthophyll, canthaxanthin, Sudan red I, Sudan red II, Sudan red III and Sudan red IV are 0.2 mu g / kg; the detection limit of riboflavin is 0.2 mu g / kg. The adding amount of the seven pigments is within the range of 2-10 mu g / kg, the recovery rate is 80.2-106.7%, and relative standard deviation RSD is 1.5-6.0%.

The invention relates to the technical field of electrochemical transducers, and in particular to a preparation method and application of an electrochemical transducer for fast detecting Sudan red in food. The method comprises the following steps: with nano ferroferric oxide-graphene material as an electrode material, ramming and loading the electrode material in an electrode tube, and inserting a copper wire to form the Sudan red electrochemical transducer. Therefore, the structure of a traditional Sudan red detector is simplified; in combination, with the application in chilli products, the sensitivity of the electrochemical transducer is improved by reasonably combining the electrode raw materials while controlling technical parameters in the detection step, so that the reliability of the Sudan red content detection result is improved, the cost of detection on the content of Sudan red in food is reduced, and the influence of food subjected to environment pollution and polluted by lawless persons to the human body health can be avoided.

The invention discloses a method for separating and enriching Sudan red series coloring agents in a large-volume environment water sample. The method comprises the following steps: synthesizing a hydrophilic polystyrene(STY)-divinylbenzen (DVB)- ethylene glycol dimethacrylate (EGDMA) high internal phase emulsion polymerization monolithic column through a high internal phase emulsion polymerization technology, wherein a volume ratio of the STY to the DVB to the EGDMA to span80 is 6:3:3:4, and the volume ratio of a water phase is 90-92%; allowing 500-1000mL of an environment water sample to go through the high internal phase emulsion polymerization monolithic column at a flow velocity in a range of 1-2mL / min, and extracting and enriching a trace amount of the Sudan red series coloring agents in the sample; cleaning the monolithic column with a 10% or 20% aqueous acetonitrile solution, eluting the monolithic column with acetone, and collecting the obtained eluate; and drying the collected eluate, and re-dissolving the dried eluate in acetonitrile to obtain a solution to be detected in order to complete separation and enrichment of the Sudan red series coloring agents in the environment water sample.

The invention relates to Sudan red I crystal A. Crystal Sudan red I exists in a polymorphic form with a main crystal form and other multiple auxiliary crystal forms, and the polymorphic form is identified through X-ray powder diffraction. The invention further provides a preparation method of the Sudan red I crystal A; the purity of the Sudan red I crystal A is greatly improved, the content of the Sudan red I crystal A is greatly increased, a prepared sample meets the technical index for detection of Sudan red I in a food as an external marker, and a special Sudan red I standard substance for food detection is further prepared.

The invention discloses a method for detecting Sudan red I in an aqueous phase, fluorescence test paper and a preparation method and application of the fluorescence test paper. The detection method comprises the following steps: (1) preparing a fluorescence probe solution, wherein the fluorescence probe solution is an amphipathic chitosan derivative CMCs-g-PPDO / PFO nanocomposite fluorescence probe solution, and the micelle concentration is 0.5-2mg / mL; (2) adding the fluorescence probe solution into a cuvette, and arranging the cuvette in a fluorescence spectrophotometer for scanning a fluorescence spectrum; (3) adding a to-be-detected sample solution into the cuvette containing the fluorescence probe solution in the step (2), arranging the cuvette in the fluorescence spectrophotometer for scanning a fluorescence spectrum, identifying Sudan red I pigments according to the fluorescence quenching degree, if the fluorescence spectrum quenching is obvious, the sample contains the Sudan red I pigments, and if the fluorescence spectrum does not have an obvious change, the sample does not contain the Sudan red I pigments. Furthermore, functional fluorescence test paper is prepared. The fluorescence test paper has a good detection effect on Sudan red I.

The invention discloses a rapid extraction purification and detection method for Sudan red dye residue in food. The rapid extraction purification and detection method comprises the following steps: collecting a sample: an experimenter wears gloves to take out all articles in a package, vegetables to be detected such as chili can be firstly prepared, the surface layer of the vegetable can be cleaned by using little water, the cleaned water is collected for standing, then the surface layer of the vegetables such as the chili is scraped, and then little flesh inside the vegetables such as chili is collected and placed in a number-one empty reagent tube. The invention relates to the technical field of food safety detection. According to the rapid extraction purification and detection method for the Sudan red dye residue in food, by virtue of the cooperation of a detection tool and chemicals in a detection apparatus, a whole detection process can be operated by an ordinary person, so that the food bought at normal times can be detected by the ordinary person at home with the detection tool without using a professional detection mechanism, the use is convenient, the supervision of publicpeople for the food can be facilitated, and the food supervision can be improved.

The invention relates to an application of Sudan red for preparing an ammonium nitrate fuel oil explosive (ANFO), and an ANFO containing the Sudan red and a preparation method thereof. The invention can enable a field operator to visually ensure that the ANFO meeting the formula requirement into each blast hole through a color board, reduces the emission of toxic gas, improves the capacity for work of the explosive, obtains good blasting effect, and meets the requirement of green organization on least content of toxic gas generated after the explosive explodes.

The invention discloses Sudan red I molecularly-imprinted solid phase extraction padding. The Sudan red I molecularly-imprinted solid phase extraction padding refers to molecularly-imprinted polymer particles which is prepared in a way that Sudan red I is taken as a template molecule, acrylamide is taken as a function monomer, maleated rosin glycol acrylate is taken as a cross-linking agent, and azoisobutyronitrile is taken as an initiating agent. The solid phase extraction padding has a memory function on a three-dimensional structure of a molecule of the sudan red I, the specificity, the selective separation and the enrichment of the sudan red I are realized, the padding is filled so as to form a solid phase extraction column, and the solid phase extraction column is used for detecting the selective separation, purification and the enrichment of the Sudan red I in an extraction solution containing a sudan red I food sample; and the high sensitivity and high selectivity detection on the food sample containing the sudan red I is realized, and the method provided by the invention has the characteristics of being high in recovery ratio, small in relative standard deviation, simple and rapid to operate, high in efficiency and the like.

The invention relates to a few-layer MXene-ionic liquid droplet modified electrode as well as a preparation method and application thereof in detection of Sudan red. The preparation method comprises the steps: adding a proper amount of few-layer MXene into ionic liquid, controlling the ultrasonic temperature to be 20 DEG C or less, performing ultrasonic oscillation for 30-40 minutes, and fully mixing to obtain a few-layer MXene-ionic liquid composite material; and taking a few-layer MXene-ionic liquid composite material, coating the surface of a glassy carbon electrode with the MXene-ionic liquid composite material, and placing the glassy carbon electrode at room temperature for 20-30 minutes to enable the composite material to be fully combined with the surface of the glassy carbon electrode so as to prepare the few-layer MXene-ionic liquid droplet modified electrode. The prepared few-layer MXene-ionic liquid droplet modified electrode shows excellent electrocatalytic activity, wide linear range and low detection limit on Sudan red I. In the detection process, the electrode is easy to operate, rapid in response and high in sensitivity, and is a novel, simple and low-cost electrochemical sensor.

The invention relates to a method to detect the Sudan red in food. It adds the detecting sample and the triton X-100 into the mixture of the AgNO3, the NaOH and the ammonia to watch the solution color change to detect that if there is the Sudan red in food; also it compares to the standard sample to go on the semi-quantitative detection; next it uses the recovery method with the mark to get the actual Sudan red concentration according to the linear equation.

The invention relates to a water-based biodegradable penetrant and a preparation method thereof. The water-based biodegradable penetrant comprises food-grade carmine, a solvent, a stable neutralizer and a surfactant, the concentration of the food-grade carmine is 1.5-3.0 g / 1000 ml, the concentration of the stable neutralizer is 8.0-12.0 g / 1000 ml, the concentration of the surfactant is 1.0-1.5 g / 1000 ml, and the solvent is water. According to the present invention, the design is reasonable, the food-grade carmine is adopted as the pigment to replace the existing Sudan red, the food-grade carmine is adopted as the food-grade pigment, such that the health and the environmental protection are provided while the water-based requirement is met, the food-grade carmine is dissolved in water to form the solution with the high color contrast, the process parameter requirement of the penetration detection is met, the sensitivity is high, and five artificial defects on the type 2 reference test block can be clearly displayed; the water-based biodegradable penetrant is low in toxicity, so that the health risk of operators can be reduced.

The invention discloses a the fat-soluble azo dye detection method and kit in the food or feed, which includes the following steps: (1) Preparation for the test solution: to be examined samples using organic solvent dilution, dissolved or extraction, as evidence test solution; (2) adding the test solution of excessive oxidant; (3) adding reducing agent to remove excess oxidizer, (4) static hierarchical, according to the color of organic layer judge whether the seized samples containing Sudan, and other fat-soluble azo dyes. The detection method of this invention is low cost, specific, sensitive and simple rapid, and does not require expensive equipment, and it can be used for the scene rapid detection of food or feed whether added illegal Sudan, and other fat-soluble azo dyes. At the same time it provides an apid screening test to see whether the food or feed contain Sudan or fat-soluble azo dyes illegally.

The invention discloses a method for detecting Sudan red I in an aqueous phase, fluorescence test paper and a preparation method and application of the fluorescence test paper. The detection method comprises the following steps: (1) preparing a fluorescence probe solution, wherein the fluorescence probe solution is an amphipathic chitosan derivative CMCs-g-PPDO / PFO nanocomposite fluorescence probe solution, and the micelle concentration is 0.5-2mg / mL; (2) adding the fluorescence probe solution into a cuvette, and arranging the cuvette in a fluorescence spectrophotometer for scanning a fluorescence spectrum; (3) adding a to-be-detected sample solution into the cuvette containing the fluorescence probe solution in the step (2), arranging the cuvette in the fluorescence spectrophotometer for scanning a fluorescence spectrum, identifying Sudan red I pigments according to the fluorescence quenching degree, if the fluorescence spectrum quenching is obvious, the sample contains the Sudan red I pigments, and if the fluorescence spectrum does not have an obvious change, the sample does not contain the Sudan red I pigments. Furthermore, functional fluorescence test paper is prepared. The fluorescence test paper has a good detection effect on Sudan red I.

The invention discloses a method for simultaneously measuring seven pigments in eggs, belonging to the technical field of food safety detection. The method is to adopt DQTP / Fe 3 o 4 Organic covalent framework structure and magnetic nanocomposite materials, as a dispersed magnetic solid-phase extraction adsorbent to extract pigments in eggs, and then use ultra-high performance liquid chromatography tandem mass spectrometry UPLC-MS / MS method combined with external standard method to determine the pigments in eggs Pigment content. The invention establishes a method for simultaneously measuring the content of riboflavin, lutein, canthaxanthin and Sudan red I, Sudan red II, Sudan red III and Sudan red IV in eggs. The detection limits of lutein, canthaxanthin, Sudan Ⅰ, Sudan Ⅱ, Sudan Ⅲ and Sudan Ⅳ were all 0.2ug / kg, and the detection limit of riboflavin was 0.2ug / kg. The recoveries of seven pigments ranged from 2 to 10ug / kg, and the recoveries were 80.2 to 106.7%, and the relative standard deviations (RSD) were 1.5 to 6.0%.

The invention discloses a detecting method of red-series coloring agents in medicinal materials. The detecting method comprises the following steps: (1) standard reagents of carmine, amaranth and erythrosine are taken to be added with ethyl alcohol of 60-80%, a first standard solution is prepared, standard reagents of acid red 73, new fuchsine, 808 scarlet, Sudan I and Sudan IV are taken to be added with ethyl alcohol, and a second standard solution is prepared; (2) 1 mL of the first standard solution and 1 mL of the second standard solution in the step (1) are taken to be diluted, and a composite standard solution is obtained; (3) the composite standard solution obtained in the step (2) is taken to be injected into a liquid chromatograph to be measured through the detection wavelength of500-520 nm; and (4) a to-be-detected sample is taken to be analyzed, and suspected peaks are re-checked through a ultraviolet-visible light chromatograph. According to the detecting method, whether aproduct contains the red-series coloring agents or not can be quickly and qualitatively detected, all signal peaks are sharp and free of trailing and interference, requirements for equipment are lowered, the checkout efficiency is improved, and the detecting method has optimistic market prospects.

The invention discloses a method for simultaneous determination of a plurality of synthetic pigments in hot pot flavorings. The method comprises extraction, concentration, purification and detection of new red, amaranth, carmine, allura red, erythrosine, sunset yellow, acid red G, acid scarlet GR, Rhodamine B, Para Red, sudan I, sudan II, sudan III, sudan IV, sudan orange G, sudan red 7B and a plurality of other synthetic pigments. The method for simultaneous determination of a plurality of synthetic pigments in hot pot flavorings provided by the invention has the advantages of simple steps and high accuracy and precision.

The invention belongs to the technical field of food detection and discloses a method for measuring industrial dyes in food through a GPC-LC-MS-MS method. According to the method, an ammonium hydroxide-acetonitrile solution is adopted to extract 9 kinds of industrial dyes, namely, Sudan I-IV, Basic orange 2, Basic orange 21, Basic orange 22, Auramine O, and rhodamine B in food; a total supernatantis collected; a GPC column is adopted to remove greases and pigments in the total supernatant; and a liquid phase-series mass spectrometry instrument is adopted to perform measurement. With the detection method of the invention adopted, separate detection of samples is omitted, and the efficiency of the detection of industrial dyes in food is improved. The method has good detection accuracy.

The invention belongs to the field of food safety detection, and more specifically relates to a sudan red III semiantigen with a structure represented by formula I, and also relates to a sudan red IIIcoupling antigen, a sudan red III antibody, and a colloidal gold rapid detection device containing the sudan red III coupling antigen and the sudan red III antibody, and applications thereof. The cross reaction of the colloidal gold rapid detection device with other kinds sudan red is reduced, so that the colloidal gold rapid detection device can be used for detecting sudan red III in food accurately; cost is low; detection is rapid and accurate; and rapid on-line detection and monitoring of suspected objects can be realized.

The invention relates to an electrochemical method for detecting Sudan red I. The electrochemical method includes respectively placing an expansion graphite paste electrode which is used as a working electrode, a saturated calomel electrode which is used as a reference electrode and a platinum wire electrode which is used as an auxiliary electrode into BR (Britton-Robinson) buffer solutions which contain the Sudan red I with different concentrations and have the pH (potential of hydrogen) of 3.0, stirring and gathering for 300 seconds at the potential of 0.1V, cleaning by deionized water after standing the solution for 30 seconds; then placing a system consisting of the three electrodes in BR buffer solution with the pH of 6.0, scanning by differential-pulse voltammetry to obtain an oxidation peak current value, which has a good linear relation with the concentration of the Sudan red I, of the Sudan red I within the potential range from 0.3V to 0.9V, measuring an oxidation peak current value of sample solution by the same method and obtaining the concentration of the Sudan red I according to a standard curve and a linear regression equation. The method is simple in operation, low in detection cost, high in sensitivity and good in stability and reproducibility and is convenient and speedy, and the Sudan red I is directly detected.

The invention relates to the technical field of electrochemical transducers, and in particular to a preparation method and application of an electrochemical transducer for fast detecting Sudan red in food. The method comprises the following steps: with nano ferroferric oxide-graphene material as an electrode material, ramming and loading the electrode material in an electrode tube, and inserting a copper wire to form the Sudan red electrochemical transducer. Therefore, the structure of a traditional Sudan red detector is simplified; in combination, with the application in chilli products, the sensitivity of the electrochemical transducer is improved by reasonably combining the electrode raw materials while controlling technical parameters in the detection step, so that the reliability of the Sudan red content detection result is improved, the cost of detection on the content of Sudan red in food is reduced, and the influence of food subjected to environment pollution and polluted by lawless persons to the human body health can be avoided.

The invention discloses a standard sample of Sudan red in eggs and a preparation method of the standard sample. According to the preparation method, a mixed stock mother liquor containing the Sudan red I, the Sudan red II, the Sudan red III and the Sudan red IV is prepared firstly, then most of the egg liquid is added for dilution, the matrix matching property can be kept in the mode, and meanwhile the problem that the Sudan red is not dissolved in water and is not prone to being evenly dispersed in the egg liquid matrix is solved; and then drying is performed in a low-temperature spray drying and airflow crushing manner, so that the material is fluffy and is not easy to harden and block in the storage process, and the sample stability is better. According to the standard sample provided by the invention, commercially available eggs are directly adopted as raw materials, the raw materials are easy to obtain, other substances capable of changing matrix components are not introduced in the preparation process, the base material matching property of the standard sample and an actual tested sample is guaranteed to the maximum extent, and the standard sample is good in uniformity and stability, and can be used for measurement and calibration of food detection related instruments, instrument period check and method verification, measurement personnel examination and the like.

The invention discloses a method for synchronous detection of five colorants in hawthorn pills, comprising the following steps: (1) preparation of a blank matrix solution; (2) preparation of a mixed reference solution; (3) preparation of a sample solution; 4) Perform liquid chromatography-tandem mass spectrometry analysis; (5) Analysis of results. The five colorants are Sudan Red I, Sudan Red II, Sudan Red III, Sudan Red IV and 808 Scarlet. This method has the advantages of simple operation, high accuracy, high precision, and good stability. It can detect one or more of 5 kinds of coloring agents at the same time. After dyeing with the above five colorants, it can be detected in batches with high detection efficiency.

The present invention discloses a sudan red I, II, III and IV degradation enzyme preparation and a preparation method thereof. The preparation method comprises the following steps: (1) Coriolus unicolor strain is inoculated from a slant culture medium into a seed liquid for culture, then transferred into a triangular flask with a base liquid medium for shaking culture, fermentation liquid is filtered to obtain mycelium, the mycelium is washed with a phosphate buffer solution for a plurality of times, then the mycelium is collected for cryopreservation for standby use; (2) the mycelium is added into liquid nitrogen and ground into homogeneous powder, a phosphate buffer solution is added, and the supernatant is taken after centrifugation; and (3) the above crude enzyme solution is precipitated with 40% ammonium sulfate, the centrifuged precipitate is the enzyme product. The enzyme preparation is a product of natural origin, has the characteristics of being simple in process, suitable for large scale industrial production, and the like, can effectively degrade the sudan I, II, III and IV dyes, has the characteristics of high degradation efficiency, short degradation cycle, simple degraded solution composition, and the like, and has a good application prospect.

The invention belongs to the field of extraction of natural products, in particular to a method for removing various harmful substances in capsanthin. The method of the invention comprises the following steps: dissolving the capsanthin to be treated in an organic solvent, and then separating the hazardous substances through an organic solvent-resistant nanofiltration membrane; the organic solvent is acetone, vegetable oil extraction solvent or ethyl acetate one or more of them. The method of the invention has the advantages of simple process, high capsanthin yield, and a loss rate of less than 0.5% compared with the non-removal process. The method of the invention can remove a number of harmful components at the same time, including removing benzopyrene in capsanthin, and reducing the level of tens of ppb to <10 ppb; removing illegal dyes such as Sudan red and rhodamine B, from tens to several 100 ppb is removed to less than 10 ppb; pesticide residues are removed from tens of ppm to less than 20 ppb, meeting the quality requirements of high-end products, and the safety is greatly improved.

The invention discloses an electrochemical method for detecting Sudan red in food. The electrochemical method specifically comprises the following steps of: (1) preparing an expanded graphite paste electrode; (2) preparing a Sudan red standard solution; (3) drawing a standard curve, specifically, taking the expanded graphite paste electrode as a working electrode, a saturated calomel electrode as a reference electrode and a platinum wire electrode as a counter electrode to obtain a three-electrode system, then inserting the three-electrode system into the Sudan red standard solution, performing stirring for enriching, and performing scanning to obtain a linear regression equation, and drawing the standard curve; (4) preparing a liquid to be detected; and (5) performing detecting. The three-electrode system is simple in structure, wide in detection range and low in detection limit, can stably, quickly and accurately detect the content of the Sudan red in food, and can be repeatedly used for detecting the Sudan red.