Detecting method of red-series coloring agents in medicinal materials

Abstract

The invention discloses a detecting method of red-series coloring agents in medicinal materials. The detecting method comprises the following steps: (1) standard reagents of carmine, amaranth and erythrosine are taken to be added with ethyl alcohol of 60-80%, a first standard solution is prepared, standard reagents of acid red 73, new fuchsine, 808 scarlet, Sudan I and Sudan IV are taken to be added with ethyl alcohol, and a second standard solution is prepared; (2) 1 mL of the first standard solution and 1 mL of the second standard solution in the step (1) are taken to be diluted, and a composite standard solution is obtained; (3) the composite standard solution obtained in the step (2) is taken to be injected into a liquid chromatograph to be measured through the detection wavelength of500-520 nm; and (4) a to-be-detected sample is taken to be analyzed, and suspected peaks are re-checked through a ultraviolet-visible light chromatograph. According to the detecting method, whether aproduct contains the red-series coloring agents or not can be quickly and qualitatively detected, all signal peaks are sharp and free of trailing and interference, requirements for equipment are lowered, the checkout efficiency is improved, and the detecting method has optimistic market prospects.

Description

technical field [0001] The invention relates to an analysis method, in particular to a detection method of a red coloring agent in medicinal materials. Background technique [0002] Adding dyes to traditional Chinese medicine is an illegal addition. Chinese herbal medicines are mainly tested for red series dyes: carmine, amaranth, erythrosin, acid red 73, new fuchsin, 808 scarlet, Sudan red Ι, and Sudan red Ⅳ. For this reason, the State Food and Drug Administration has issued a number of detection methods for the dyeing of Chinese medicinal materials, but these methods are mostly for the detection of single Chinese medicinal materials (see Table 1 below). When faced with a variety of Chinese medicinal materials, due to the diversity of methods , leading to the complexity of the pre-treatment and analysis process on the computer, and the slow detection efficiency. Therefore, a detection method of red dye in Chinese medicinal materials was explored to find a general detection...

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Problems solved by technology

[0006] Most of the above detection methods are single dye detection, and the detection efficiency is low. Therefore, it is necessary to explore the method of detecting multiple components of the red series of Chinese medicinal materials at the same time, and establish a method that can detect multiple components of the red series of Chinese medicinal materials. Universal detection method for simultaneous detection of components

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