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Method for detecting sudan red in foods

A detection method, Sudan red technology, is applied in the measurement of scattering properties, color/spectral properties, and material analysis by observing the influence of chemical indicators, which can solve the problems of complicated pretreatment and expensive instruments, and achieve detection results. Good, low detection cost, easy to operate

Inactive Publication Date: 2009-04-15
SOUTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no matter which method is used, it needs to be separated by high performance liquid chromatography, the pretreatment is complicated, and the instruments used are expensive

Method used

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  • Method for detecting sudan red in foods
  • Method for detecting sudan red in foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Detection of Sudan Red Content in Commercially Available Chili Oil

[0033] 1. First determine the testing standard:

[0034] A. Standards for qualitative and semi-quantitative detection: 2.25mL 1.0×10 -3 Mix M silver nitrate solution, 0.4mL 0.1M sodium hydroxide solution and 0.25mL 0.4% ammonia water in a 10mL test tube, then add 1.0×10 -5 M Sudan red I, II, III, IV, concentration ranges are 0.2-2.4μM, 0.1-2.4μM, 0.1-2.4μM, 0.2-3.0μM, and 0.3mL 0.2% Triton X-100 solution, diluted with water to 5.0mL, mix again, and after 20min, different shades of brown solutions at different concentrations are obtained. Take photos and record the Sudan red solutions with different shades at different concentrations. When the concentration of Sudan red is small and it is difficult to observe the color change, use a laser pointer or LED irradiates the solution, and also takes pictures to record the intensity of scattered light observed, and uses the above records as the sta...

Embodiment 2

[0044] Embodiment 2: the detection of Sudan Red content in the commercially available chili sauce

[0045] The detection standard that adopts is identical with embodiment 1.

[0046] 1. Pre-treat the chili sauce first: add 1.0g of chili sauce to a test tube, dilute to 10mL with DMF and stir thoroughly to extract the Sudan Red that may be contained in it. Stir for 1 hour and let it stand for 10 minutes. The supernatant was filtered through a microporous membrane, and the filtrate was diluted 10 times before detection;

[0047] 2. Add 2.25mL 1.0×10 -3 M silver nitrate solution, 0.4mL 0.1M sodium hydroxide solution and 0.25mL 0.4% ammonia water, mix thoroughly, then add 0.02mL of the above-mentioned processed chili sauce sample, and 0.3mL 0.2% Triton X-100 solution, dilute to 5.0mL, mix again, add five groups in parallel, measure the scattering intensity after 20min, calculate the concentration of Sudan Red in the actual sample according to the linear equation, and get the "mea...

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Abstract

The invention relates to a method to detect the Sudan red in food. It adds the detecting sample and the triton X-100 into the mixture of the AgNO3, the NaOH and the ammonia to watch the solution color change to detect that if there is the Sudan red in food; also it compares to the standard sample to go on the semi-quantitative detection; next it uses the recovery method with the mark to get the actual Sudan red concentration according to the linear equation.

Description

technical field [0001] The invention relates to a detection method of Sudan red. Background technique: [0002] At present, the standard method for detecting Sudan Red announced by the European Union is the detection method (HPLC / APCI-MS method) of "high performance liquid chromatography coupled with electrospray ionization mass spectrometer". The detection steps of the method are that the actual sample is first extracted with acetonitrile, filtered, separated by high-performance liquid chromatography, and then quantitatively detected by mass spectrometry. In addition, other detection methods such as "high performance liquid chromatography-ultraviolet detection method" (HPLC-UV method) are used. The previous steps are similar to those announced by the European Union, except that a UV spectrophotometer is used for the final quantitative detection. There are also "HPLC-DAD" detection methods, etc. However, no matter which method is used, high performance liquid chromatograph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/25G01N21/49
Inventor 黄承志武丽萍
Owner SOUTHWEST UNIV
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