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Enzyme preparation, preparation method and application thereof in sudan red degradation

An enzyme preparation, Sudan red technology, applied in the field of bioengineering and environmental pollutant treatment, can solve the problem of less research on degradation methods

Active Publication Date: 2016-01-27
五峰杰成魔芋食品有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Sudan Red mainly focuses on the analysis and detection of Sudan Red content in food, but there are few studies on the degradation method of Sudan Red in the environment. The related reports are TiO2 photocatalytic degradation and a microorganism of Fusarium acuminate degradation

Method used

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  • Enzyme preparation, preparation method and application thereof in sudan red degradation
  • Enzyme preparation, preparation method and application thereof in sudan red degradation
  • Enzyme preparation, preparation method and application thereof in sudan red degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The enzyme preparation of the present invention can be obtained from the fermented mycelia of the microorganism Coriolus unicolor. Specific steps are as follows:

[0022] 1. Seed culture

[0023] (1) Preparation of seed medium: Cut 200g of peeled potatoes into small pieces, add appropriate amount of water to boil for 30min, filter with gauze, add 20g of glucose to the filtrate, make up 1L of tap water, and evenly distribute them in ten 250mL Erlenmeyer flasks Medium (about 100mL / one Erlenmeyer flask), sterilize at 121°C for 20min.

[0024] (2) Seed culture: Place the Coriolus unicolor strains on a slant medium for activation and then place them in an aseptic operating environment, use an inoculation needle to draw a 0.5cm×0.5cm bacterial block and inoculate it on the pre-set seed The culture medium was placed in a shaker flask with a rotation speed of 120 r / min, and incubated at a constant temperature of 28° C. for 4 days to obtain a seed liquid.

[0025] 2. Fermenta...

Embodiment 2

[0032] The enzyme preparation of the present invention is used for degrading one or more of Sudan Red I, Sudan Red II, Sudan Red III and Sudan Red IV. Specific steps are as follows:

[0033] (1) Take 600 mg of the above enzyme preparation and dissolve it in 50 mL of 50 mM phosphate buffer. Take 0.5mL of enzyme solution, dilute it to 1mL with 50mM phosphate buffer, take 0.2mL respectively and add to the solutions of Sudan Red I, II, III, and IV, and the final concentration of Sudan Red I, II, III, and IV in the solution is 10 μg / mL , the volume of the reaction solution was 2 mL.

[0034] (2) The reaction solution was incubated on a shaker at 30°C for 2 days at a constant temperature, and the final concentrations of Sudan I, II, III, and IV in the reaction solution were detected by HPLC.

[0035] (3) According to the final quality of Sudan Red in the reaction solution, calculate the Sudan Red degradation rate.

[0036] figure 1 Chromatograms of Sudan Red I, Sudan Red II, Sud...

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Abstract

The present invention discloses a sudan red I, II, III and IV degradation enzyme preparation and a preparation method thereof. The preparation method comprises the following steps: (1) Coriolus unicolor strain is inoculated from a slant culture medium into a seed liquid for culture, then transferred into a triangular flask with a base liquid medium for shaking culture, fermentation liquid is filtered to obtain mycelium, the mycelium is washed with a phosphate buffer solution for a plurality of times, then the mycelium is collected for cryopreservation for standby use; (2) the mycelium is added into liquid nitrogen and ground into homogeneous powder, a phosphate buffer solution is added, and the supernatant is taken after centrifugation; and (3) the above crude enzyme solution is precipitated with 40% ammonium sulfate, the centrifuged precipitate is the enzyme product. The enzyme preparation is a product of natural origin, has the characteristics of being simple in process, suitable for large scale industrial production, and the like, can effectively degrade the sudan I, II, III and IV dyes, has the characteristics of high degradation efficiency, short degradation cycle, simple degraded solution composition, and the like, and has a good application prospect.

Description

technical field [0001] The invention relates to an enzyme preparation and a preparation method thereof, and applies the enzyme preparation to degrade Sudan red, belonging to the fields of bioengineering and environmental pollutant treatment. Background technique [0002] Sudan Red is a lipophilic azo chemical dyeing agent (non-food additive), mainly used for dyeing oil paint, engine oil, wax and shoe polish, etc. It does not exist naturally in food, mainly including I, II, III and IV Four types. Such substances are mutagenic and carcinogenic, and have obvious toxic effects on the liver and kidney organs of the human body. They are prohibited from being used in food in my country. Therefore, the presence of Sudan Red in the environment directly affects human health. At present, the research on Sudan Red mainly focuses on the analysis and detection of Sudan Red content in food, but there are few studies on the degradation method of Sudan Red in the environment. The related r...

Claims

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Application Information

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IPC IPC(8): C12N9/02A62D3/02C12R1/645A62D101/28
CPCA62D3/02A62D2101/28C12N9/0059C12Y110/03001
Inventor 邓张双周金山陈良立施亚群
Owner 五峰杰成魔芋食品有限责任公司
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