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Method for detecting fat-soluble azo dyes in food or fodder and its kit

An azo dye and detection method technology, which is applied in the field of food hygiene detection, can solve the problems of expensive instruments, missed detection of instrument analysis methods, and high analysis costs, and achieve the effects of reducing analysis costs, simple and fast operation, and improving inspection efficiency

Inactive Publication Date: 2007-11-14
广东省疾病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In April 2005, the No. 43 announcement issued by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China pointed out: "According to the World Health Organization's International Agency for Research on Cancer (IARC) in 1995, Sudan Red belongs to the third class of carcinogens. It can Causes cancer in mice and rabbits, but no direct evidence of carcinogenicity in humans
Its disadvantages are: (1) the instrument is expensive, the sample pretreatment time is long, the chromatographic column is easily polluted, and the analysis cost is high; (2) when the chromatographic retention time of the coexisting components is close to that of the target component, it is easy to make mistakes Judgment; (3) The instrument has high requirements for the use environment and needs to be placed fixedly, which is not suitable for on-site inspection with large fluidity; (4) High technical requirements for inspectors
Limited by factors such as chromatographic resolution, coexisting interfering substances, and standards, instrumental analysis methods can generally only screen a few or ten or twenty target compounds at a time, but there are many types of fat-soluble azo dyes that can be used for illegal addition , when the added dye is not in the preset range, the instrument analysis method will miss detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Chili oil 1 (commercially available bottled, the chili oil is orange-red, known to contain 0.3mg of Sudan I per mL)

[0057] Step 1: Take about 0.2 mL of chili oil with a straw, put it in a stoppered test tube, add 0.8 mL of cyclohexane (the purpose of adding cyclohexane is to reduce the viscosity of the oil, so that it is easy to split into Small droplets, increase the oxidation reaction contact area, so that the colored interfering substances in the oil are oxidized faster);

[0058] Second step: add weight percent concentration and be 10% sulfuric acid solution 5mL (the purpose of adding sulfuric acid is to improve the oxidation potential of potassium permanganate, make it have enough oxidation capacity, improve the speed of oxidation reaction);

[0059] Step 3: Add 1 mL of potassium permanganate solution with a molar concentration of 0.1 mol / L, shake vigorously for about 5 minutes, and let it stand for 2 minutes. The water layer in the test tube is still obviously p...

Embodiment 2

[0064] Chili powder 1 (commercially available in bulk, red in color, detected by high performance liquid chromatography, known to contain no Sudan I, Sudan II, Sudan III, Sudan IV, Sudan Orange G, Sudan Red 7B, Sudan Red G, Para Red, etc. dye).

[0065] Step 1: Take about 2 grams of chili powder, put it in a 50mL colorimetric tube, add 10mL of water, then add 8mL of cyclohexane, shake vigorously for 5 minutes, and absorb 1mL of the supernatant with a straw after the layers are separated (orange-yellow) was used as the test solution, and placed in another test tube; the remaining operations were in accordance with the second to fifth steps in Example 1.

[0066] In this experiment, it was observed that the organic layer obtained in the fifth step was clear and colorless, so it was judged that the sample did not contain fat-soluble azo dyes.

Embodiment 3

[0068] Paprika 2 (get the paprika in embodiment 2 in right amount, add Sudan I standard solution, mix thoroughly, get the positive sample that every gram sample contains Sudan I0.3mg).

[0069] All operations are the same as in Example 2.

[0070] In this experiment, it was observed that the organic layer obtained in the last step was orange-yellow, so it was judged that the sample probably contained fat-soluble azo dyes.

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Abstract

The invention discloses a the fat-soluble azo dye detection method and kit in the food or feed, which includes the following steps: (1) Preparation for the test solution: to be examined samples using organic solvent dilution, dissolved or extraction, as evidence test solution; (2) adding the test solution of excessive oxidant; (3) adding reducing agent to remove excess oxidizer, (4) static hierarchical, according to the color of organic layer judge whether the seized samples containing Sudan, and other fat-soluble azo dyes. The detection method of this invention is low cost, specific, sensitive and simple rapid, and does not require expensive equipment, and it can be used for the scene rapid detection of food or feed whether added illegal Sudan, and other fat-soluble azo dyes. At the same time it provides an apid screening test to see whether the food or feed contain Sudan or fat-soluble azo dyes illegally.

Description

technical field [0001] The invention relates to the field of food hygiene detection, in particular to a detection method and a kit for fat-soluble azo dyes such as Sudan red in food or feed. Background technique [0002] At present, there are thousands of chemically synthesized dyes. Azo dyes account for about two-thirds of the total. Azo dyes are one of the most numerous and diverse synthetic dyes, and are widely used in industry. Azo dyes can be divided into fat-soluble azo dyes (or lipophilic azo dyes) and water-soluble azo dyes (or hydrophilic azo dyes). Azo compounds do not occur naturally in food. Due to the strong toxicity of most azo dyes, few of them can be used as food additives. In my country, only a few water-soluble dyes such as tartrazine are allowed to be used as food additives, and there are strict regulations on their dosage. Fat-soluble azo dyes are prohibited from being added to food. [0003] The use of coloring in food can change the "appearance" o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25
Inventor 朱炳辉龙朝阳高燕红钟志雄
Owner 广东省疾病预防控制中心
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