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Rapid detection method of lafil PDE5 type inhibitor in drugs, health foods and foods

A detection method and inhibitor technology, applied in the direction of analysis using chemical indicators, can solve problems such as unfavorable on-site operations, and achieve the effects of wide application range, high accuracy, and strong specificity

Active Publication Date: 2011-07-27
广东省药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above method is simple to operate, it needs to use strong oxidizing and corrosive concentrated sulfuric acid and highly toxic chloroform, and needs to be evaporated in a water bath at 80°C or blown dry with a hair dryer, which is not conducive to on-site operation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Lafite-like PDE added in medicine, health food and food of the present invention 5 A preferred embodiment of the detection method of type inhibitor, comprises the steps:

[0038] (1) Take solid or liquid medicine, health food or food, add 1ml of ethyl acetate as an extraction solvent, shake for 2 minutes, let stand for 10 minutes, and take the supernatant;

[0039] (2) Color reaction: Add 1 drop of the supernatant of the step (1) into 0.5 ml of 30% dilute sulfuric acid by weight, and observe whether purple is produced.

[0040] If the solution is purple within 10 minutes, it is preliminarily judged to contain diaphane chemical components.

Embodiment 2

[0042] Lafite-like PDE added in a kind of medicine, health food and food of the present invention 5 Another preferred embodiment of the detection method of type inhibitor, comprises the steps:

[0043] 1. Preparation of test solution

[0044] Take 1 capsule (1 tablet; about 1 / 2 of the dosage of solid preparations such as pills, granules, powders, etc.), and put it in a stoppered test tube (tablets and pills need to be ground or chopped in advance) , add 2ml of ethanol, shake for about 1 to 2 minutes, let stand for about 1 minute, take the supernatant, that is.

[0045] 2. Color reaction

[0046] Add 1ml of dilute sulfuric acid with a concentration of 45% by weight in the test tube, add 2 drops of the test solution, and observe whether purple is produced.

[0047] 3. Result judgment

[0048]If the test solution is purple within 2 minutes, it is preliminarily judged to contain tadalafil chemical components.

Embodiment 3

[0050] Lafite-like PDE added in medicine, health food and food of the present invention 5 A preferred embodiment of the detection method of type inhibitor, comprises the steps:

[0051] (1) Take solid or liquid medicine, health food or food, add 20ml of extraction solvent acetone, shake for 1 minute, let stand for 5 minutes, and take the supernatant;

[0052] (2) Color reaction: Add 1-2 drops of the supernatant of the step (1) to 10 ml of perchloric acid to observe whether purple is produced.

[0053] If the solution is purple within 10 minutes, it is preliminarily judged to contain diaphane chemical components.

[0054] In several other preferred embodiments, the extraction solvent is selected from isopropanol and methanol, and the color developer is selected from 80% dilute sulfuric acid, hydrochloric acid and nitric acid.

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PUM

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Abstract

The invention discloses a rapid detection method of a lafil PDE5 type inhibitor in drugs, health foods and foods, comprising the following steps: taking solid or liquid drugs, health foods or foods, adding 1-20ml of extraction solvent, shaking the solvent for 1-2 minutes, standing for 1-10 minutes, and then taking supernatant; enabling 1-2 drops of the supernatant to react with 0.5-10ml of a colour developing agent, if the solution is purple in 2 minutes, initially judging that lafil chemical composition is contained. The method has the advantages of simple operation, strong specificity, high sensitivity, high accuracy and no requirement on special equipments, thereby avoiding using agents with relatively high toxicity or strong corrosion, being suitable for field operations, and being capable of meeting the supervision and inspection demands of primary level units, vast rural areas and small-medium cities with relatively backward detection technology conditions.

Description

technical field [0001] The invention relates to the technical field of analysis of medicines, health food or food, in particular to a rapid screening of diaphane-like PDEs added in medicines, health food or food based on physical and chemical reactions 5 methods for the detection of inhibitors. Background technique [0002] Illegal addition of chemical ingredients in Chinese patent medicines for nourishing kidney and strengthening yang, health food and food is becoming more and more rampant. Illegal addition of chemical ingredients is relatively common, and there are more and more high-tech counterfeiting activities aimed at standards, which seriously threaten the health of the people. After reviewing a large number of domestic and foreign literature, it was found that the illegally added aphrodisiac chemical ingredients are mainly PDE 5 Type inhibitors (phosphodiesterase type-5 inhibitor, phosphodiesterase type-5 inhibitor) are the main ones. The U.S. Food and Drug Admini...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N31/22
Inventor 罗卓雅魏嘉陵林生文赖宇红雷毅李延志
Owner 广东省药品检验所
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