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Method for detecting Sudan red I in aqueous phase, fluorescence test paper and preparation method and application thereof

A technology for detecting Sudan red and water, which is applied in the fields of optics and polymer chemistry, can solve problems such as increasing the cost of detection, restricting detection of Sudan Red I, etc., and achieves the effects of improved sensitivity, good water solubility, and simple and easy preparation process

Active Publication Date: 2014-06-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Conjugated polymers with special fluorescent signal amplification function are excellent sensing active materials, and have sensitive recognition ability for electron-deficient Sudan I. However, the hydrophobicity of these conjugated polymers limits their use in aqueous phase. Application of Detection of Sudan Red I in the System
Therefore, modifying these conjugated polymers to make them hydrophilic can expand their application range. However, the preparation of water-soluble conjugated polymers involves a very complicated synthesis process, which greatly increases the cost of detection.

Method used

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  • Method for detecting Sudan red I in aqueous phase, fluorescence test paper and preparation method and application thereof
  • Method for detecting Sudan red I in aqueous phase, fluorescence test paper and preparation method and application thereof
  • Method for detecting Sudan red I in aqueous phase, fluorescence test paper and preparation method and application thereof

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Embodiment 1

[0039] 5g of purified chitosan (Mw=50000) was added to 50mL of saturated NaOH solution with a mass fraction of 50%, stirred evenly and placed in the refrigerator overnight; the swollen sample was transferred to a 1000mL beaker, and 300mL of iso Propanol was stirred evenly; 5g of chloroacetic acid was added to the beaker in batches, and mechanically stirred for 12 hours; the isopropanol was filtered out, and water was added to dissolve the sample; the pH of the solution was adjusted to neutral with dilute acetic acid, and dialyzed in deionized water for 3-5d (MWCO= 3500); the solution in the dialysis bag was collected and freeze-dried for 48 hours to obtain CMCs; 10g of ionic liquid [Bmim]Cl was heated and melted, and 0.2g of dry CMCs was added, N 2 Under the protection of 1500rmp magnetic stirring for 3h to completely dissolve the CMCs; N 2 Add 0.388g p-dioxanone (PDO) and 0.128g 4-dimethylaminopyridine (DMAP) to the solution under protection, N 2 Under protection, react at 1...

Embodiment 2

[0041] The CMCs-g-PPDO prepared in Example 1 was formulated into a 0.9 mg / mL aqueous solution of polymer nanomicelles, and 1 mL of a 0.5 mg / mL PFO / THF solution was slowly added dropwise to 10 mL of an aqueous solution of polymer nanomicelles with stirring. Ultrasound for 2 hours to mix evenly, remove THF by rotary evaporation, add water to 10mL; filter with 0.45 μm microporous membrane to remove unembedded PFO to obtain the amphiphilic chitosan derivative CMCs-g-PPDO / PFO nanocomposite Fluorescent probe solution. The loading rate of the conjugated polymer PFO in the probe solution was measured and calculated by an ultraviolet spectrometer to be 11.36%; 2.5 mL of the probe solution diluted 5 times was placed in a four-sided quartz cuvette, and at an excitation wavelength of 340 nm, a narrow Scan the fluorescence spectrum under the condition of a slit width of 2.5nm; drop 10 μL of the sample solution to be tested into the cuvette containing the fluorescent probe solution, scan it...

Embodiment 3

[0043] The CMCs-g-PPDO prepared in Example 1 was formulated into a 1.35 mg / mL aqueous solution of polymer nanomicelles, and the particle size was determined to be 23.18 nm by dynamic light scattering (DLS) using a Malvern particle size analyzer; 100 μL 0.5 mg / mL PFO / THF solution was slowly added dropwise to 10mL polymer nanomicelle aqueous solution under stirring condition, ultrasonically mixed for 2h, then rotary evaporated to remove THF, added water to make up to 10mL; filtered with 0.45μm microporous membrane to remove unembedded PFO That is, the amphiphilic chitosan derivative CMCs-g-PPDO / PFO nanocomposite fluorescent probe solution is obtained. The loading rate of the conjugated polymer PFO in the probe solution was measured and calculated by an ultraviolet spectrometer to be 41.94%; 2.5 mL of the probe solution diluted 5 times was placed in a four-sided quartz cuvette, and the excitation wavelength was 340 nm, narrowly Scan the fluorescence spectrum under the condition o...

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Abstract

The invention discloses a method for detecting Sudan red I in an aqueous phase, fluorescence test paper and a preparation method and application of the fluorescence test paper. The detection method comprises the following steps: (1) preparing a fluorescence probe solution, wherein the fluorescence probe solution is an amphipathic chitosan derivative CMCs-g-PPDO / PFO nanocomposite fluorescence probe solution, and the micelle concentration is 0.5-2mg / mL; (2) adding the fluorescence probe solution into a cuvette, and arranging the cuvette in a fluorescence spectrophotometer for scanning a fluorescence spectrum; (3) adding a to-be-detected sample solution into the cuvette containing the fluorescence probe solution in the step (2), arranging the cuvette in the fluorescence spectrophotometer for scanning a fluorescence spectrum, identifying Sudan red I pigments according to the fluorescence quenching degree, if the fluorescence spectrum quenching is obvious, the sample contains the Sudan red I pigments, and if the fluorescence spectrum does not have an obvious change, the sample does not contain the Sudan red I pigments. Furthermore, functional fluorescence test paper is prepared. The fluorescence test paper has a good detection effect on Sudan red I.

Description

technical field [0001] The invention relates to a preparation method and application of an optical material carboxymethyl chitosan grafted p-dioxanone / fluorescent dye composite nano-micelle and a functionalized test paper for detecting Sudan Red I, belonging to optics and In the field of polymer chemistry, it is expected to be used to detect the safety of food additives. Background technique [0002] In recent years, the rapid, efficient and sensitive detection of the carcinogenic pigment additive Sudan Red I has become the focus of attention in the global food safety field. At present, the detection of Sudan Red I mainly relies on relatively expensive detection instruments, and the detection is time-consuming, such as mass spectrometry and chromatography. However, in many cases, especially in critical situations, it is often necessary to quickly make a judgment on the content of Sudan Red I on the spot, but in many methods that can be used to detect Sudan Red I, there are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 王小慧陈惠孙润仓黄飞
Owner SOUTH CHINA UNIV OF TECH
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