33 results about "Phenotypic screening" patented technology

Provided is a system for identifying a hydrogen gas producing organism. The system includes a sensor film having a first layer comprising a transition metal oxide or oxysalt and a second layer comprising a hydrogen-dissociative catalyst metal, the first and second layers having an inner and an outer surface wherein the inner surface of the second layer is deposited on the outer surface of the first layer, and a substrate adjacent to the outer surface of the second layer, the organism isolated on the substrate.

The present invention provides a method for identifying a functional antibody or antigen-binding protein or a fragment thereof that is capable of binding to, and stimulating the activity of, a targettransmembrane protein comprising the steps of providing yeast cells transformed with yeast expression vectors encoding a library of antibodies or antigen-binding proteins or fragments thereof, whereinsaid yeast cells express said target transmembrane protein, expressing said library of antibodies or antigen-binding proteins or fragments thereof in the yeast cells, wherein said expressed antibodies or antigen-binding proteins or fragments thereof are secreted into the periplasmic space of said yeast cells, incubating the yeast cells in or on a selective medium or under a restrictive temperature, or a combination thereof, and detecting a predetermined phenotype in the yeast cells, wherein detection or manifestation of the predetermined phenotype is indicative of binding of the functional antibody or antigen-binding protein or a fragment thereof to said target transmembrane protein and stimulation of said target transmembrane protein by the functional antibody or antigen-binding proteinor a fragment thereof. Antibodies or antibody fragments that are agonists of G-protein coupled receptors identified by the method of the invention are also provided.

The invention provides a high-yield crossbreeding method of two-line super rice parent GuiKe-2S stock seeds. The high-yield crossbreeding method comprises the following steps: A, carrying out phenotypic screening on a GuiKe-2S large group, namely, planting a GuiKe-2S group of a single plant; B, detecting a specificity marker of a GuiKe-2S sterile locus; C, detecting a molecular marker of a GuiKe-2S fingerprint spectrum; D, carrying out low-temperature screening on a GuiKe-2S first-time manual climatic box; E, carrying out low-temperature screening on a GuiKe-2S second-time manual climatic box; and F, breeding a rice root in winter of Hainan, transplanting the GuiKe-2S the root of which is cut again to Sanya of Hainan for breeding in winter, and harvesting seeds when ripening to obtain the two-line super rice parent GuiKe-2S stock seeds. According to the high-yield crossbreeding method, the stock seeds are further bred to obtain high-purity parent seeds applied to seed production, which has a very important significance in developing two-line hybrid rice. Through tests, the purity of the obtained breeder seeds reaches above 99.99 percent, and the purity of the stock seeds obtained through expanding propagation reaches above 99.9 percent.

The invention discloses a method for accurately and rapidly identifying a drug target of an active compound obtained by phenotypic screening. The seamless joint of compound phenotypic screening and target identification is established by a chemical proteomics-driven caenorhabditis elegans feeding RNAi technology; the active compound is an active compound with a corresponding phenotype in the elegans; the phenotype includes but is not limited to lipid dysbolism, cell apoptosis, autophagy, senility, neurodegenerative disease and anti-infection. The problem that at present, false positive and false negative are easily produced when the proteomics method is purely used for analyzing the interacting protein of drug can be solved, and the aim of eliminating the false and retaining the true can be realized; furthermore, the method is simple and convenient in operation and rapid and accurate in screening, and provides a feasible scheme for the analysis of the large-flux interacting protein of the drug; a more perfect chemical proteomics technology system is formed, and the possibility of breaking through the choke point of phenotypic screening protein target identification is provided.

The invention provides a breeding method of a multiple-birth mutton sheep breed and relates to the technical field of animal breeding. The breeding method herein is characterized in that a Hu sheep, as a first male parent, an east eastfrierian sheep as a second female parent and a Tan sheep as a female parent are subjected to three-breed crossing; the novel multiple-birth mutton sheep breed suitable for the ecological conditions of loess plateau is cultivated through phenotypic screening in combination with screening of specific gene carriers; the multiple-birth mutton sheep breed is adaptiveto the natural ecological conditions of the loess plateau ecological region of China and has the advantages of good stress resistance, crude feeding resistance, high propagation rate, good meat tenderness, uniform fat distribution, little odor and the like.

Described herein are methods and compositions for high efficiency transfection of siRNA into a cell population. Such methods and compositions utilize a low voltage pre-conditioning pulse to modulate the efficiency of siRNA transfection. In some embodiments, the methods and compositions permit spatial and temporal control of siRNA transfection efficiency within a population of cells. The disclosed methods and compositions, in some embodiments, are amenable to high throughput applications such as siRNA library-based phenotypic screening.

The present invention includes a method, kits, and assays for identifying a human subject as having an increased risk of developing an autoimmune disease, or a human subject with multiple sclerosis caused by elevated soluble Interleukin 7 receptor (sIL7R), by obtaining a biological sample and detecting or measuring in the biological sample an amount of a soluble Interleukin-7 receptor (sIL7R) and an amount of an RNA Helicase DDX39B, whereby a lower expression of DDX39B and a higher secretion of sIL7R identifies the subject from which the biological sample was obtained as having an increased risk of developing an autoimmune disease, when compared to a human subject not having an autoimmune disease. The present invention also includes a method of modifying a treating of subjects based on the lower expression of RNA Helicase DDX39B alone or in combination with an increase in sIL7R.

The invention belongs to the field of wild plant resource utilization research, in particular to a method for breeding glyphosate-resistant new germplasm by using wild soybeans. The method for breeding new glyphosate-resistant / tolerant germplasm uses different concentrations of glyphosate to treat seedling wild soybeans to screen out wild soybeans with resistance / tolerance to glyphosate, and then use the selected wild soybeans with resistance / tolerance The wild soybean and the cultivated soybean are interspecifically crossed to obtain interspecific hybrid progeny, and the inbred lines of the interspecific hybrid progeny are screened for glyphosate resistance / tolerance and combined with phenotypic screening to select glyphosate-resistant / new germplasm of tolerance. The present invention determines the wild soybean resource resistance standard evaluation system, establishes a method for screening wild soybeans with resistance / tolerance, uses it for interspecific hybridization, and creates a method for breeding new glyphosate-resistant / tolerant germplasm , the method is simple, practical and efficient, and is also suitable for the study of other traits of wild soybean.