Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method of constructing a neural tube defect model and its application

A neural tube deformity and construction method technology, applied in the field of animal model construction, can solve the problem of unclear neural tube deformity and other problems

Active Publication Date: 2021-06-04
首都儿科研究所
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many studies have found that elevated homocysteine ​​(homocysteine, HCY) levels caused by abnormal folic acid metabolism is one of the important risk factors for birth defects, and the mechanism of high homocysteine ​​leading to neural tube defects is still unclear. unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method of constructing a neural tube defect model and its application
  • A method of constructing a neural tube defect model and its application
  • A method of constructing a neural tube defect model and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The following materials are mainly used in this embodiment:

[0027] White Laihang chicken eggs (China Agricultural University, China); Homocysteine ​​Thiolactone hydrochloride (Sigma, USA); Tyrode Buffer; Phenol Red (Beijing Chemical Plant); Incubator (Showa Furanki, Japan). The main instruments include optical and dissecting microscopes, automatic incubators, micro-injectors, dental drills and egg openers, etc.

[0028] The specific preparation method of the neural tube defect animal model is as follows:

[0029] Collect artificially inseminated White Legion breeder eggs, select eggs with smooth surface, oval shape, and moderate size, wash off the mucus and feces on the surface with water, wash with 0.1% bromogeramine and put them in the incubator for incubation. The incubation condition is the incubation temperature 38°C, 55-65% relative humidity, 90° rotation angle, automatic egg turning at intervals of 30 minutes, and continued incubation. This time is recorded a...

Embodiment 2

[0035] In order to explore the effect of 0.5mM HTL neural groove injection on chicken embryo neural tube defects, the inventors expanded the sample size and injected 0.5mM HTL neural groove with an injection volume of 5 μl. The control group was injected with bench-top liquid containing phenol red. figure 1 shown. according to figure 2 At the key time points of neural tube development shown, embryos were taken for observation at E2 before neural tube closure, E3, E5 and E8 after neural tube closure.

[0036] At the E2 stage of embryonic development, the malformation rate of embryos was 59.1%, with abnormal brain development and abnormal closure of neural grooves, and some embryos were accompanied by abnormal body shape. At E3, brain development was obviously abnormal, and the deformity rate was 50%. Spina bifida, brachycephaly and abnormal body segment development appeared, and the stillbirth rate was 9.09%. At E5, the malformation rate of embryos was 56.6%, mainly brain dy...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for constructing a neural tube defect model, which comprises the following steps: step 1, collecting artificially inseminated eggs, cleaning them and placing them in an incubator for incubation; step 2, incubating the eggs to the neural plate of chicken embryos After the start of closure, inject homocysteine ​​thiolactone into the eggs using the chicken embryo neural groove injection method; step 3, continue to incubate the treated eggs, observe the phenotype of the chicken embryos, and screen out successful neural tube defects Model. Another aspect of the present invention also discloses the application of the above construction method in the study of the pathogenesis of neural tube defects caused by high homocysteine. By adopting the neural tube defect model construction method provided by the invention, the deformity rate of the nervous system reaches 50%, and the embryo survival rate reaches 87.5%, which is currently the best modeling method for observing neural tube defects.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a construction method and application of a neural tube defect model. Background technique [0002] Neural tube defects (NTDs) are serious congenital malformations of the central nervous system, and the incidence rate in northern my country, especially in Shanxi Province, can be as high as 10.2‰. The etiology of neural tube defects is complex. Many studies have shown that neural tube defects are caused by the interaction of genes and environmental factors, such as environment, nutrition, metabolism, organic solvents, etc., are closely related to the occurrence of neural tube defects. Many studies have found that elevated homocysteine ​​(homocysteine, HCY) levels caused by abnormal folic acid metabolism is one of the important risk factors for birth defects, and the mechanism of high homocysteine ​​leading to neural tube defects is still unclear. unclear. [000...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/381A61P25/00
CPCA61K31/381A61P25/00
Inventor 张勤张霆白宝玲李丹万春雷
Owner 首都儿科研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com