A method for breeding new germplasm with resistance/resistance to glyphosate using wild soybean

Abstract

The invention belongs to the field of wild plant resource utilization research, in particular to a method for breeding glyphosate-resistant new germplasm by using wild soybeans. The method for breeding new glyphosate-resistant / tolerant germplasm uses different concentrations of glyphosate to treat seedling wild soybeans to screen out wild soybeans with resistance / tolerance to glyphosate, and then use the selected wild soybeans with resistance / tolerance The wild soybean and the cultivated soybean are interspecifically crossed to obtain interspecific hybrid progeny, and the inbred lines of the interspecific hybrid progeny are screened for glyphosate resistance / tolerance and combined with phenotypic screening to select glyphosate-resistant / new germplasm of tolerance. The present invention determines the wild soybean resource resistance standard evaluation system, establishes a method for screening wild soybeans with resistance / tolerance, uses it for interspecific hybridization, and creates a method for breeding new glyphosate-resistant / tolerant germplasm , the method is simple, practical and efficient, and is also suitable for the study of other traits of wild soybean.

Description

technical field [0001] The invention belongs to the field of wild plant resource utilization research, in particular to a method for breeding glyphosate-resistant new germplasm by using wild soybeans. Background technique [0002] Glyphosate is a very important organophosphorus herbicide. It is a foliar, broad-spectrum, non-selective herbicide that has strong control over annual and perennial weeds. [0003] The glyphosate-resistant gene is transferred into the transgenic soybean, so that the sensitivity of the transgenic soybean to glyphosate is relatively low, and the shikimic acid pathway in the transgenic soybean plant can proceed normally, thus showing resistance to glyphosate. Since GM soybeans were approved for commercial production in the United States in 1994, the global acreage of GM soybeans has increased rapidly. However, due to the large-scale planting of glyphosate-resistant transgenic soybeans and the long-term and single use of glyphosate, the frequency of g...

AI Technical Summary

Problems solved by technology

However, due to the large-scale planting of glyphosate-resistant transgenic soybeans and the long-term and single use of glyphosate, the frequency of glyphosate-resistant weeds in the field has increased significantly; or other herbicides, the transgenic soybean plants will not be able to quickly decompose glyphosate, thus appearing symptoms of phytotoxicity; at the same time, during the selection of resistant varieties, some varieties will be less resistant to glyphosate than other varieties, As a result, genetically modified soybeans have phytotoxicity problems after spraying glyphosate

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