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Cultivation method of polyploidy of triptonia macranthum

A cultivating method and a technology for moss brightleaf, applied in the field of cultivating polyploidy of brightleaf moss, can solve the problems of unsatisfactory breeding, low chromosomes, and bryophytes have not yet been applied, and achieve improved breeding efficiency, stable polyploid genome, The effect of increasing the number of false roots

Active Publication Date: 2021-10-08
丽水市润生苔藓科技有限公司
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Problems solved by technology

Polyploid breeding has two ways of artificially inducing chromosomal doubling and selecting mutants with natural chromosomal doubling, but the frequency of natural chromosomal doubling is low, and the probability of polyploid breeding in natural populations is low, which is far from satisfying Breeding needs, and artificial mutagenesis has the advantages of high efficiency and rapidity, so most of the existing polyploid breeding adopts the method of artificially inducing chromosome doubling
Currently, this method has not been applied to bryophytes

Method used

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  • Cultivation method of polyploidy of triptonia macranthum
  • Cultivation method of polyploidy of triptonia macranthum
  • Cultivation method of polyploidy of triptonia macranthum

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preparation example Construction

[0033] The invention uses a colchicine solution to soak the moss brightphyllum protonema to obtain the mutagenic moss brightphyllum protonema. In the present invention, it is preferable to use the sterile moss brightphyllum protonema obtained from tissue culture as a material for the colchicine solution treatment, and using the moss brightphyllum protonema as a mutagenic material can shorten the breeding time. In the present invention, the protocelium of Physcomitrella aciniphyllum is preferably young vigorously growing protocelium of Physcomitrella aciniphyllum; the preparation method of the protocelium of Moss aciniphyllum preferably includes the following steps: selecting robust mature After the spore capsules of Physcomitrella phyllosum were sterilized, the spores germinated, and the obtained aseptic protonema were cultured for 7 days, and then subcultured twice in succession to obtain young and tender protocelia of Physcomitrella brightoli. In the present invention, the m...

Embodiment 1

[0042] (1) The aseptic protonema obtained by tissue culture of healthy Physcomitrella brightoli was selected, and the obtained protonema was continuously subcultured once after the protonema was cultured for 7 days. The subculture steps are as follows: scrape about 0.1 g of the protonema of Physcomitrella brightoli grown on the surface of the medium with a sterile spoon, mix it with 10 mL of sterile water, and grind it with a homogenizer to make a moss suspension. The grinding parameters are 10s / time, 10 times / material, the moss suspension was inoculated on a new medium for culture, and the protonema fragments in the suspension regenerated the protonema.

[0043] (2) The preparation steps of the colchicine mother solution are as follows: accurately weigh 1 g of colchicine with an analytical balance, place the colchicine in a sterile blue cap reagent bottle, and add 100 μL of dimethyl sulfoxide (Dimethyl sulfoxide, DMSO), add 100mL sterile distilled water, stir to dissolve so t...

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Abstract

The invention relates to a cultivation method of polyploidy of triptonium nigrum, and belongs to the technical field of polyploidy induction. The method disclosed by the invention comprises the following steps of soaking the leucotinum leucotinoides protonemata by using a colchicine solution to obtain mutagenized leucotinum leucotinoides protonemata; carrying out continuous subculture on the mutagenized hypnum macranthum protonemata for three times to obtain subcultured protonemata; carrying out ploidy identification on gametophytes differentiated from the subcultured protonemata to obtain genomic doubled mutants; carrying out subculture on the genome doubled mutant for five times, and screening out a genome doubled and stably inherited mutant; and observing the phenotype of the mutant with doubled genome and stable inheritance, and screening the triptonia melanoides mutant with a brand new phenotype as a new triptonia melanoides strain. The cultivation method is simple to operate, the occurrence rate of chimeras can be reduced, and the obtained polyploidy is stable in heredity.

Description

technical field [0001] The invention relates to the technical field of polyploid induction, in particular to a method for cultivating polyploids of Physcomitrella brightoli. Background technique [0002] There are many kinds of bryophytes. There are about 23,000 kinds of bryophytes in the world, including 15,000 kinds of mosses, 8,000 kinds of liverworts, and 100 kinds of hornworts. Most of the bryophytes are tiny, with a length of only a few millimeters to a few centimeters, and the largest can reach 100 centimeters, with a relatively simple structure. The bryophytes have different shapes. Some mosses grow in dense clusters and form cushions, some mosses prostrate and grow intertwined into sheets, and some mosses grow upright like trees. Using the characteristics of mosses combined with other gardening techniques for landscaping can create ancient Mossy landscape full of natural beauty. In addition, moss has strong adaptability and is not easy to cause diseases and insect...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H11/00A01G22/30
CPCA01H1/08A01H11/00A01G22/30
Inventor 刘莉李青温从发黄钧超
Owner 丽水市润生苔藓科技有限公司
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