64 results about "Pelteobagrus eupogon" patented technology

The invention discloses a swelling granular mixed feed for adult pelteobagrus fulvidraco, relating to the field of pelteobagrus fulvidraco feed. The feed is suitable for feeding the pelteobagrus fulvidraco in adult stage (namely the pelteobagrus fulvidraco in the adult stage with the weight of more than 20g); the feed is a mixture prepared by all raw materials according to the processing method of swelling granular feeds; according to the weight percentage, the feed comprises the following raw materials of: 36-40% of fish meal, 15.58-19.6% of flour, 3.5-4% of cottonseed meal, 16-20% of soybean meal, 6-7% of maize protamine, 4.5-5.5% of fish oil, 6-7% of beer yeast, 1-1.5% of mineral matters, 0.5-0.6% of choline chloride and 0.2% of multivitamin. Compared with normal mixed feed, the swelling granular mixed feed has the advantages of comprehensive nutrition, high livability, little pollution on culture water, greenness, and no public nuisance.

The invention relates to a cultivation method for the summer fingerling of the pelteobagrus. The method comprises the following steps of: (1) selecting a cultivation pond; (2) selecting a water source; (3) preparing the cultivation pond; (4) reasonably stocking; (5) managing the feeding; (6) managing the quality of the water; and (7) preventing and curing the disease and the like. When the method is used for cultivating the summer fingerling of the pelteobagrus, the total artificial forage domestication technologies with different types and different modes are adopted at different times by means of combining with the mode such as eel material slurry sprinkling, dried power sprinkling, bean blocking by powder cluster and the like according to the biologic learnability in different periods to provide a most effective practical measure and method for cultivating the summer fingerling of the pelteobagrus so as to perfect the cultivation technology for the summer fingerling of the pelteobagrus, firstly and successfully break through the key point of the total artificial forage cultivation of the summer fingerling of the pelteobagrus in the domestics, have a theoretical significance and a practice guidance significance for cultivating the artificial forage of the pelteobagrus and improving the forage use ratio, and lay a technical foundation for cultivating the fingerling on a large scale.

The invention discloses a Pelteobagrus fulvidraco and an interbreeding species identification method thereof based on a depth convolution generation antagonistic network, belonging to the technical field of machine learning. The method comprises the following steps of: collecting original pictures of yellow catfish and interbreeding fish of yellow catfish, and generating fish pictures similar to the original pictures through a generated antagonistic network; the fish images are mixed with the original images and sent to the convolution neural network for training. The trained convolution neural network was used to identify Pelteobagrus fulvidraco and its interbreeds. The method can generate images similar to the real Pelteobagrus fulvidraco and its interbreeding species under the conditionof very few samples, and thoroughly solves the problem that Pelteobagrus fulvidraco living in water is hard to collect images out of water, and has hard spines on the body surface, which leads to manual sorting and easy injury. By using the original data and the pictures generated by the depth convolution countermeasure network for the input of the convolution neural network, the recognition accuracy of the network model is significantly improved, and the final recognition accuracy can reach 94.2%, which has important application value.

The present invention discloses a SNP molecular marker associated with growth traits of Pelteobagrus vachelli, which is located at 41cM position of linkage group 14 of Pelteobagrus vachelli. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO: 1, and a SNP site exists at the 26th position of the gene sequence: the base is G or C. The SNP molecular marker is located at 41cM position of linkage group 14 of Pelteobagrus vachelli. The invention also discloses a specific primer, a kit and a detection method for detecting the molecular marker. It is found that, The genotype ofthe SNP site at 26 bp of the gene at 41 cM in linkage group 14 of Pelteobagrus vachelli is GG, GC or CC is closely related to growth traits of Pelteobagrus vachelli, such as body weight, body width and total length. Therefore, the SNP molecular marker of the invention can be used for detecting or assisting in detecting growth traits of Pelteobagrus vachelli, and can be used for assisting selectionand breeding of Pelteobagrus vachelli. The invention has important guiding significance for breeding new strain of excellent growth characteristics of Pelteobagrus vachelli.

The invention discloses high-protein feed for promoting feeding and digesting of juvenile fish of pelteobagrus fulvidraco. The high-protein feed comprises brown fishmeal, bean pulp, beer yeast, corn gluten meal, rapeseed dregs, cottonseed meal, wheat bran, wheat middling, dried blood, carboxymethylcellulose, shrimp shell meal, corn gluten meal, corn, soya-bean oil, lecithin, rapeseed oil, radix astragali, radix codonopsis, rehmannia glutinosa, radix angelica sinensis, fructus crataegi, malt, choline chloride, pigment, composite bacillus, phagostimulant, vitamin premix, composite multi-ore, complex enzyme and antioxidant. The feed for the juvenile fish of the pelteobagrus fulvidraco is high in protein content and can promote feeding and digesting of the juvenile fish of the pelteobagrus fulvidraco, raise the yield of the pelteobagrus fulvidraco and improve quality of the pelteobagrus fulvidraco.

The invention discloses a compound health-caring feed for pelteobagrus fulvidraco and a preparation method thereof. The compound health-caring feed for the pelteobagrus fulvidraco comprises the following ingredients in the following percentage: 10-30% of red snapper meal, 20-35% of soybean meal, 1-5% of fermented soybean meal, 3-10% of rapeseed meal, 3-10% of cottonseed meal, 20-25% of high-gluten flour, 1.0-3.0% of soybean lecithin, 1.0-3.0% of soybean oil; 1.0-3.0% of fish oil, 0.1-0.5% of choline chloride; 1-3% of fish soluble paste, 1.0-2.0% of calcium dihydrogen phosphate, 0.1-0.3% of a mildew proofing agent, 1-4% of beer yeast, 0.5-2% of yeast hydrolysate, 0.1-0.5% of betaine, 0.05-0.2% of chitin; 0.2-0.5% of a vitamin premix for aquaculture, and 1.0-2.0% of a trace element premix for aquaculture. The compound health-caring feed for the pelteobagrus fulvidraco disclosed by the invention is wide and stable in raw material sources, so that preparation cost of the feed prepared by the preparation method is relatively low; moreover, the feed is capable of meeting all nutritional requirements of pelteobagrus fulvidraco cultivation. In addition, the feed is high in feed conversion rate so that rapid growth of the pelteobagrus fulvidraco is ensured; and thus, breeding benefits are high. The feed prepared by the preparation method is also capable of obviously enhancing immune functions and anti-stress abilities of the pelteobagrus fulvidraco fed with the feed; and thus, the adult pelteobagrus fulvidraco is good in vitality, natural in body color and high in survival rate.

The invention discloses a tor brevifilis and pelteobagrus fulvidraco mixed aquaculture method. The tor brevifilis and pelteobagrus fulvidraco mixed aquaculture method includes the following steps: A, selecting, repairing and maintaining a fishpond; B, cleaning and disinfecting the fishpond, wherein the fishpond is dried, flattened and exposed, and then disinfection is carried out through quick lime; C, injecting and enriching water, wherein thoroughly-decomposed organic fertilizers are evenly scattered into the fishpond; D, breeding fries, wherein at the end of April, when the water temperature is higher than or equal to 10 DEG C, young pelteobagrus fulvidracos are bred; in the beginning of June, young tor brevifilises are bred; before breeding is carried out, the fries are soaked in a potassium permanganate solution with the concentration ranging from 18 mg / L to 22 mg / L for 10 minutes to 15 minutes; E, managing feeding, wherein the everyday feeding amount is 3% to 4% of the weight of all the fishes during the period from April to June, the everyday feeding amount is 6% to 10% of the weight of all the fishes during the period from June to October, and feeding is not carried out when the water temperature is higher than or equal to 30 DEG C or the air temperature is lower than to equal to 10 DEG C; F, carrying out daily management. The tor brevifilis and pelteobagrus fulvidraco mixed aquaculture method can meet the requirements of people in multiple aspects, a water area and feed are reasonably used, and the yield of the tor brevifilises and the yield of the pelteobagrus fulvidracos are increased.

The invention relates to the field of aquatic feed processing, and discloses pelteobagrus fulvidraco culturing feed and a preparation method thereof. The feed provided by the invention is prepared from Peru fish meal, bean pulp, flour, soya-bean oil, monocalcium phosphate, zeolite powder, saltwater fish compound premix, feed grade calcium propionate, vitamin C and an antioxidant. The pelteobagrus fulvidraco culturing feed provided by the invention adopts multiple proper components, fish oil commonly used in a traditional feed formula is removed, meanwhile, a cold spraying technology of cooling the feed first and then spraying grease is adopted for preparation, the prepared feed can reduce the feed coefficients and increase the specific growth rate and relative weight gain rate of pelteobagrus fulvidraco.

The invention discloses a sex identification method for juvenile fish of hybrid pelteobagrus fulvidraco (richardson) [pelteobagrus fulvidraco (richardson) (male)* pelteobagrus vachelli (richardson) (female)]. The hybrid pelteobagrus fulvidraco (richardson) having an individual body length of above 3 cm is identified by utilization of a method of living-body gonad squashing, acetocarmine staining and ethanol crystal violet staining, and the accuracy of the method is verified by a paraffin section of the gonad tissue. The sex identification method is suitable for sex identification for juvenile fish of common pelteobagrus fulvidraco (richardson) and of "all-male No.1" pelteobagrus fulvidraco (richardson). By the sex identification method, sex identification of one juvenile fish can be completed in 3 min, and 100 fishes can be identified by cooperation of two persons for 2 h. The sex identification method has characteristics of high efficiency, economy, rapidness, accuracy and stability. The sex identification method has important application value for male fry cultivation of the pelteobagrus fulvidraco (richardson) and the hybrid pelteobagrus fulvidraco (richardson) thereof, and can be applied for rapid sex identification of a pelteobagrus fulvidraco (richardson) commodity summer fry.

The invention provides a breeding method of excellent strains of hybrid pelteobagrus fulvidraco resistant to Edwardsiella ictaluri.The method comprises the following steps: collecting the pelteobagrusfulvidraco and pelteobagrus vachelli, cultivating till mature, and then reproducing to obtain the fry of purebred pelteobagrus fulvidraco and the pelteobagrus vachelli with different populations; soaking all the fingerlings of the pelteobagrus fulvidraco and the pelteobagrus vachelli with a soaking method according to determined medial lethal concentration of the Edwardsiella ictaluri; performingbreeding on the population resistant to the Edwardsiella ictaluri twice, screening the excellent disease-resistant strains by the survival rate of mixed-cultured fry, respectively cultivating the strains of the pelteobagrus fulvidraco resistant to the Edwardsiella ictaluri, and the pelteobagrus vachelli to sexual maturity, and then producing the hybrid pelteobagrus fulvidraco according to a universal artificial reproduction method of fish. According to the breeding method of the excellent strains of the hybrid pelteobagrus fulvidraco resistant to the Edwardsiella ictaluri, hole-in-head-disease-resisitant parents of the pelteobagrus fulvidraco and the pelteobagrus vachelli can be cultivated, the incidence and mortality of hole-in-head disease in the seedling and culture process are improved, and the survival rate of the culture is improved.

The invention discloses a pelteobagrus fulvidraco beta defensin gene, and beta defensin antibacterial peptide and application thereof. An in vitro recombinant expression technology is used for successfully expressing a pelteobagrus fulvidraco beta defensin recombinant protein PET-32a-Pf_BD, and antibacterial activity identification results show that the pelteobagrus fulvidraco beta defensin gene has a certain bacteriostasis for staphylococcus aureus in gram-positive bacterium, escherichia coli in gram-negative bacterium, aeromonas hydrophila, edwardsiella ictaluri and flavobacterium columnare. Therefore, a foundation is laid for application of the defensin recombinant protein, used as a broad-spectrum antibacterial drug, in aquaculture.

The invention relates to the technical field of biology and discloses a whole genome cloning method of pelteobagrus fulvidraco bacillus-shaped virus. The method comprises the following steps of: designing 14 specific primers for reverse transcription and 14 pairs of specific primers for PCR, wherein the primer sequences are shown as SEQ ID No.1-SEQ ID No.14; extracting pelteobagrus fulvidraco bacillus-shaped virus RNA; taking the extracted viral RNA as a template, respectively adding the extracted viral RNA into a reverse transcription reaction system and a PCR reaction system by using the primers, cloning the whole genome of the pelteobagrus fulvidraco bacillus-shaped virus by sections under reaction conditions, wherein 13 fragments of the amplified product are 2000bp in size, one fragment is 1000bp in size, a total of 14 fragments contain the entire genome sequence of the pelteobagrus fulvidraco bacillus-shaped virus. The invention provides a cloning method of the whole genome of thepelteobagrus fulvidraco bacillus-shaped virus. The method not only can be used for rapid cloning of the whole genome of the pelteobagrus fulvidraco bacillus-shaped virus, but also can be used for researching molecular biology pathogenic mechanism and molecular epidemiology investigation of the pelteobagrus fulvidraco bacillus-shaped virus, and is convenient to use and has good effect.

The invention discloses primers for rapidly identifying peltebagrus fulvidraco, pelteobagrus vachelli and hybrid peltebagrus fulvidraco and an identification method of the peltebagrus fulvidraco, the pelteobagrus vachelli and the hybrid peltebagrus fulvidraco. A nucleotide sequence of an inad-like gene of the peltebagrus fulvidraco is represented as SEQ ID No.1. On the basis of sequence difference of the Inad-like gene in genomes of the peltebagrus fulvidraco and the pelteobagrus vachelli, a pair of markers capable of accurately identifying the peltebagrus fulvidraco, the pelteobagrus vachelli and the hybrid peltebagrus fulvidraco is screened out, and foundation is laid for further breeding research of the peltebagrus fulvidraco.

The invention relates to an SRAP molecular marker, a kit and an identification method for identifying Pelteobagrus fulvidraco, Pelteobagrus vachelli and first filial generation of the Pelteobagrus fulvidraco and the Pelteobagrus vachelli and application, and belongs to the technical field of hybrid variety breeding of aquaculture. Amplification primers of the SRAP molecular marker are a forward primer Me3 with a nucleotide sequence as shown in SEQ ID NO.1 and a reverse primer Em3 with a nucleotide sequence as shown in SEQ ID NO.2. The molecular marker provided by the invention can be used forsimply and accurately distinguishing the Pelteobagrus fulvidraco, the Pelteobagrus vachelli and the first filial generation, so that the parent breeding and fry breeding effects are improved.

The invention relates to a low-fish-meal compound feed for preventing and treating enteritis of pelteobagrus fulvidraco and a preparation method of the low-fish-meal compound feed. The low-fish-meal compound feed comprises the following components in parts by weight: 21-24 parts of fish meal, 6-10 parts of corn protein powder, 12-14 parts of flour, 5-7 parts of chicken meal, 1-2 parts of blood cell protein powder, 4-6 parts of pork powder, 5-7 parts of cottonseed protein powder, 22-27 parts of soybean meal, 6-10 parts of rapeseed meal, 3.6-3.9 parts of soybean oil, 2-6 parts of a premix and 0.1-2 parts of an intestinal function compound. Wherein the intestinal function compound is prepared from the following components in parts by weight: 0.1 to 0.3 part of sodium butyrate, 0.05 to 0.2 part of citric acid, 0.05 to 0.2 part of glutamine and 0.05 to 0.2 part of protease. The low-fish-meal compound feed can ensure the growth function under the condition of low fish meal, the feed cost isreduced, and the culture benefit is improved.

The invention relates to a method for increasing pelteobagrus fulvidraco fry breeding survival rate and a preparation method thereof. The preparation method comprises the following steps: (1) designing a fry breeding pond, (2) designing a bait breeding pond, (3) seeding, absorbing excessive nutrients and fixing the bottom of the fry breeding pond and the bait breeding pond, (4) fertilizing and water culture, (5) throwing fries, (6) controlling diseases and insect pests, (7) supplementing biological baits, (8) feeding feeds and (9) regularly regulating water. The net frame is erected on the periphery of the fry breeding pond, the aeration stones are arranged on the net frame, attachments are provided for fries which just go down to the pond and cannot swim horizontally, and the fries are prevented from sinking into the water bottom and being killed by oxygen deficit.

The invention relates to a feeding device, in particular to an automatic feeding device for pelteobagrus fulvidraco culture. According to the automatic feeding device for pelteobagrus fulvidraco culture, when feed is fed, the amount of the feed falling each time is consistent, and the feed cannot be caked. The automatic feeding device for pelteobagrus fulvidraco culture comprises a pulling rope and a storage mechanism; a floating mechanism is connected to one end of the pulling rope, and the storage mechanism is arranged on the floating mechanism. Through the floating mechanism and the storagemechanism, the device can float around under the action of water flow, and meanwhile, the feed leaks, so that the condition that a large amount of pelteobagrus fulvidraco is piled together to snatchthe feed is avoided; and the feed can be prevented from being solidified in a storage box through a driving mechanism and a stirring mechanism.

The invention discloses a preparation method of N4-acetyl-sulfamethoxazole residue standard substance in pelteobagrus fulvidraco tissue. The preparation method includes: A, selecting female pelteobagrus fulvidraco which is determined without containing sulfamethoxazole and N4-acetyl-sulfamethoxazole; B, using dimethyl formamide to dissolve sulfamethoxazole, and using water for dilution to obtain a sulfamethoxazole solution; C, orally pouring the sulfamethoxazole solution according to dosage of 60 mg / kg body weight of pelteobagrus fulvidraco, and putting pelteobagrus fulvidraco after going through oral administration in water for observation; D, selecting pelteobagrus fulvidraco without vomiting to prepare N4-acetyl-sulfamethoxazole residue standard substance, collecting back muscle, liver, kidney, gill and ovary of pelteobagrus fulvidraco for homogenization respectively to prepare the N4-acetyl-sulfamethoxazole residue standard substance in the pelteobagrus fulvidraco tissue, freezing and storing the same. The preparation method is easy to implement, simple and convenient to operate and capable of valuing the standard substance prepared and testing uniformity and stability.

The present invention discloses a compound feed used for improving immunity of pelteobagrus fulvidraco and reducing NO2- in water body and an application thereof. The compound feed is characterized byconsisting of fish meal, soybean meal, flour, blood globulin powder, corn protein powder, expanded soybeans, bran, monocalcium phosphate, peanut meal, soybean oil, carboxymethyl cellulose, zeolite powder, a premix and daphnia magna powder. The fish meal, daphnia magna powder and soybean meal account for 45%-55% of the total mass of the compound feed, a mass ratio of the fish meal to soybean mealis (1.4-1.6):1, and a mass ratio of the fish meal to the daphnia magna powder is 0.15-2.4.

The invention discloses a preparation for improving fish intestinal pathology injury. The preparation disclosed by the invention is prepared from the following components in parts by weight: 1 part ofmangosteen extract and 3 parts of stevia rebaudian extract. When the preparation is added into a feed in proportion or mixed with the feed for use, the preparation has remarkable repairing and improving effects on damaged intestinal tracts of cultured animals and has no adverse effect on the production performance of aquatic animals. The preparation is added into feed according to the proportionof 0.1%, so that the intestinal injury of pelteobagrus fulvidraco caused by histamine can be remarkably repaired; the intestinal health condition of the ricefield eel fed by the iced fresh fish can beobviously improved by mixing and feeding the preparation according to the proportion of 0.3%. The preparation is wide in raw material source, convenient and easy to purchase, easy to obtain and low in cost; the preparation is simple to prepare and operate without special instruments and equipment; and the preparation is simple and easy to use, high in applicability, free of residues after being used and environmentally friendly.

The invention discloses a dual-gRNA site amh gene knockout method in pelteobagrus fulvidraco. The method comprises the following steps of (1) designing a target site 1 on the first exon of a pelteobagrus fulvidraco amh gene, and designing a target site 2 on the fourth exon; (2) according to a target site point sequence design primer in the step (1), detecting the accuracy of the target site in parent fish, amplifying the target site 1 and a near sequence by using amh E1 F and amh E1 R, and amplifying the target site 2 and the near sequence by using amh E4 F and amh E4 R; (3) using a pUC19-gRNA-scaffold plasmid as a template, performing PCR amplification on a gRNA1 fragment by using amh E1 gRNA F and gRNA R, and performing PCR amplification on a gRNA2 fragment by using amh E4 gRNA F and gRNA R, wherein the PCR products are used as the template, in vitro transcription is performed, and purification is performed, so that gRNA is obtained; (4) using a pXT7-hCas9 linearization plasmid as atemplate, and performing in vitro transcription to synthetize Cas9 mRNA; (5) performing microinjection on Cas9 mRNA and two gRNA into a cell stage embryo of pelteobagrus fulvidraco; and (6) detectingthe mutation type, and calculating the gene editing rate. The invention further discloses an application of the method.

The invention provides a feed formula for promoting growth of pelteobagrus fulvidraco and improving the utilization rate and a processing technology of the feed formula, and relates to the technical fields of aquaculture feed and preparation. The feed formula is prepared from the following raw materials in parts by weight: 20-30 parts of fish meal, 15-25 parts of soybean meal, 2-4 parts of blood meal, 8-12 parts of rapeseed meal, 20-30 parts of flour, 2-3 parts of soybean oil, 2-3 parts of fish oil, 1-2 parts of calcium dihydrogen phosphate, 0.2-1.0 part of a vitamin premix, 2-7 parts of a mineral salt premix, 0.01-0.05 part of ethoxyquinoline, and 3-7 parts of cellulose. The feed formula has the following beneficial effects: the feed formula can effectively shorten the culture cycle of the pelteobagrus fulvidraco, improve the growth rate and feed utilization rate of the pelteobagrus fulvidraco, and improve the survival rate of the pelteobagrus fulvidraco.

The invention discloses a plteobagrus fulvidraco feed for different breeding stages and application of the pelteobagrus fulvidraco feed. According to breeding scales, breeding time, market requirements and the like of pelteobagrus fulvidraco, pelteobagrus fulvidraco breeding stages are divided into a recovery stage, a rapid growth stage (also called as rapid growth early stage), a high temperaturestage, a new fish rapid growth stage (also called as rapid growth later stage) and a wintering period; and according to stage division of the plteobagrus fulvidraco, and together with research achievements of the plteobagrus fulvidraco, nutrition standards of the plteobagrus fulvidraco at different stages are confirmed, and the problems that stage feed standards are nor reasonably designed in breeding of the plteobagrus fulvidraco and nutrition standards are not matched with different specifications in breeding of the plteobagrus fulvidraco, can be solved.

The invention provides a feed additive for preventing and treating bacterial disease of pelteobagrus fulvidraco. The feed additive comprises the following raw materials in parts by weight of 20-26 parts of poria cocos, 16-24 parts of honeysuckle flowers, 25-35 parts of a dendrobium huoshanense extract, 6-12 parts of cortex eucommiae essential oil and 10-16 parts of a carrier, wherein the carrier is modified Maifan stone powder. The feed additive can effectively restrain growth of harmful pathogenic bacteria, and is notable in effects of preventing and treating bacterial gill diseases.

The invention discloses a feed for preventing pelteobagrus fulvidraco from body grayish white patches The feed comprises the following components of fish meal, hydrolyzed fish protein powder, soybeanprotein concentrate, spray-dried blood cell protein powder, beer yeast, meat and bone meal, fermented soybean meal, corn protein powder, seaweed meal, flour, corn, soybean oil, soybean phospholipid oil, monocalcium phosphate, vitamin C phosphate, vitamin A, vitamin D and a premix additive. The invention further discloses a preparation method and application of the feed at the same time. The feed can effectively enhance the immunity of the pelteobagrus fulvidraco, prevent the occurrence of body grayish white patch diseases or body rotting diseases, and also has a good treatment effect on the pelteobagrus fulvidraco suffering from the body grayish white patch diseases or the body rotting diseases; and meanwhile, the culture water environment can be improved, and livers and intestines of thepelteobagrus fulvidraco are protected, so that the culture survival rate is improved, and the culture benefits is increased.

The invention belongs to the technical field of fish heredity nutriology, and particularly relates to pelteobagrus fulvidraco larva fish high in vegetable protein tolerance and a cultivating method ofthe pelteobagrus fulvidraco larva fish high in vegetable protein tolerance. According to the method, the principle combining heredity nutriology and biological breeding science is adopted, through experiment, a technical system for cultivating pelteobagrus fulvidraco fry varieties high in vegetable protein tolerance is obtained. Pelteobagrus fulvidraco larva fish after 3-15dph of incubation and film outlet are fed with a compounded high-vegetable protein inducing feed, appropriate-duration vegetable protein feed short time stimulus is given (feeding stimulus duration of the high vegetable protein feed is between 5-15 days), and pelteobagrus fulvidraco juvenile fish fry varieties which do not have significant differences from a non-stimulus group in specific growth rate, are higher than the non-stimulus group in nonspecific immunity capacity with digestive absorption capacity or do not have significant differences from the non-stimulus group in the nonspecific immunity capacity and thedigestive absorption capacity can be obtained. In the respect of feed cost, through the high-level vegetable protein, the feed cost can be significantly reduced, the production cost can be saved, andthe breeding benefits can be increased.

The invention relates to a pelteobagrus fulvidraco feed additive, a pelteobagrus fulvidraco feed and a preparation method of the pelteobagrus fulvidraco feed, and relates to the field of aquatic feeds. The pelteobagrus fulvidraco feed additive comprises at least two of a compound Chinese herbal medicine extract, a lactobacillus plantarum seed solution and a bacillus subtilis seed solution. The pelteobagrus fulvidraco feed is prepared from the following raw material substances in percentage by weight: 32-34% of soybean meal, 18-20% of wheat flour, 3.5-4.2% of soybean oil, 1.8-2.2% of monocalcium phosphate, 0.25-0.32% of a mineral trace element additive, 0.23-0.26% of composite vitamins, 0.18-0.21% of choline chloride, 0.08-0.11% of a mildew inhibitor, 0.045-0.052% of an antioxidant, 0.01-1.01% of a pelteobagrus fulvidraco feed additive and the balance of fish meal. The preparation method of the pelteobagrus fulvidraco feed comprises the following steps of S1, solid material cleaning, S2, primary crushing, S3, primary blending, S4, secondary crushing, S5, secondary blending and S6, blending processing. The feed has the advantages of being free of antibiotics, remarkable in antibacterial and disease-resistant effects and balanced in nutrition.

The invention discloses pelteobagrus fulvidraco feed and a feeding method thereof which have the benefits that pelteobagrus fulvidraco is fed by using an insect protein product specially obtained by taking insect protein dry powder as a raw material as well as other special ingredients and by using warm mineral spring water of 27-29 DEG C containing specific ingredients, so that the zero additionof antibiotics and hormones in the feeding process can be realized, the disease resistance of the pelteobagrus fulvidraco is improved, and the zero occurrence of disease damage caused by ichthyophthirius and other parasites can be ensured; meanwhile, the culture time of the pelteobagrus fulvidraco can be shortened by 20 percent or more, and the yield per mu can reach 2000 kilograms or more. Therefore, the economic benefits are significant.

The invention discloses a large-scale breeding method for hybrid pelteobagrus fulvidraco. The large-scale breeding method comprises the following steps: pond selection and cleaning: cleaning a pond, selecting the area and the trend of the breeding pond, cleaning the pond by mixing quick lime and water, removing sludge by using a bulldozer or sucking sludge by using a sludge pump, exposing the bottom of the pond to the sun, and drying the bottom of the pond to crack; building of fish nests: building multiple rows of fish nests in the pond through reeds or branches, arranging the multiple rows of fish nests on one side of the pond in the east-west direction and arranging aerators on bank sides; fry releasing: scattering fries, after the internal and external water temperatures are adapted, opening fry oxygen bags, and slightly scooping pond water into the fry oxygen bags; bait putting and guarantee of water quality. Compared with the prior art, the method has the advantages that multiple rows of fish nests are built in the pond, oxygen is increased through air pumps, and fry death caused by oxygen deficit during fry gathering can be prevented; and water in the pond is used for washing the outer surface of a foam box during fry releasing, so that the temperature difference inside and outside the oxygen bags of the foam box is balanced, the inside and outside water bodies are mixed and buffered, and the survival rate of fries is increased.

The invention relates to a pelteobagrus fulvidraco fingerling feed with comprehensive nutrition, which is characterized by being prepared from the following components in percentage by weight: 31 to 40 percent of fish meal, 25 to 35 percent of bean cake, 10 to 15 percent of corn, 5 to 10 percent of bran, 5 to 10 percent of flour, 3 to 5 percent of vegetable oil, 1 to 2 percent of yeast, 1 to 2 percent of tapioca flour, 0.5 to 1 percent of a premix and 0.5 to 1 percent of monocalcium phosphate. The compound feed is comprehensive in nutrition, good in palatability and beneficial to growth of pelteobagrus fulvidraco.