Primers for rapidly identifying peltebagrus fulvidraco, pelteobagrus vachelli and hybrid peltebagrus fulvidraco and identification method of peltebagrus fulvidraco, pelteobagrus vachelli and hybrid peltebagrus fulvidraco

A technology for yellow catfish and yellow catfish, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, animal/human peptides, etc., and can solve the problem of infertility, inability to prepare from seed, and it is difficult to distinguish hybridization Problems of yellow catfish and common yellow catfish fry and adult fish

Active Publication Date: 2017-02-22
HUAZHONG AGRI UNIV
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Problems solved by technology

However, offspring of hybrid yellow catfish are generally infertile and cannot be used for later seed preparation
[0004] At present, it is difficult to distinguish the hybrid yellow catfish from the common yellow catfish fry and adults morphologically

Method used

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  • Primers for rapidly identifying peltebagrus fulvidraco, pelteobagrus vachelli and hybrid peltebagrus fulvidraco and identification method of peltebagrus fulvidraco, pelteobagrus vachelli and hybrid peltebagrus fulvidraco
  • Primers for rapidly identifying peltebagrus fulvidraco, pelteobagrus vachelli and hybrid peltebagrus fulvidraco and identification method of peltebagrus fulvidraco, pelteobagrus vachelli and hybrid peltebagrus fulvidraco
  • Primers for rapidly identifying peltebagrus fulvidraco, pelteobagrus vachelli and hybrid peltebagrus fulvidraco and identification method of peltebagrus fulvidraco, pelteobagrus vachelli and hybrid peltebagrus fulvidraco

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Embodiment Construction

[0025] In order to better explain the present invention, the main content of the present invention is further clarified below in conjunction with specific examples, but the content of the present invention is not limited to the following examples.

[0026] Method for identification of yellow catfish, tile style yellow catfish and hybrid yellow catfish

[0027] 1) Take 10 yellow catfish, 10 yellow catfish and their hybrids, and sample them at the Shangshehu Lake Breeding Base in Jiangxia District, Wuhan City.

[0028] 2) Genomic DNA extraction of yellow catfish, tile style yellow catfish and their hybrids

[0029] Take 0.2g fin ray tissue into a 1.5ml centrifuge tube, add 600μl cell lysate (Tris-HCl 100mM, pH 8.0; EDTA 50mm / L, pH 8.0; SDS 1%, pH 8.0; NaCl 125mM) and 15μl proteinase K (10mg / ml), shred the fin rays in a centrifuge tube, place the centrifuge tube in a water bath at 62°C for 2-4 hours, shake the centrifuge tube every half hour until fully lysed. Take out the cent...

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Abstract

The invention discloses primers for rapidly identifying peltebagrus fulvidraco, pelteobagrus vachelli and hybrid peltebagrus fulvidraco and an identification method of the peltebagrus fulvidraco, the pelteobagrus vachelli and the hybrid peltebagrus fulvidraco. A nucleotide sequence of an inad-like gene of the peltebagrus fulvidraco is represented as SEQ ID No.1. On the basis of sequence difference of the Inad-like gene in genomes of the peltebagrus fulvidraco and the pelteobagrus vachelli, a pair of markers capable of accurately identifying the peltebagrus fulvidraco, the pelteobagrus vachelli and the hybrid peltebagrus fulvidraco is screened out, and foundation is laid for further breeding research of the peltebagrus fulvidraco.

Description

technical field [0001] The invention relates to a molecular marker technology, in particular to a primer for quickly identifying yellow catfish, tile-style yellow catfish and hybrid yellow catfish and an identification method thereof. Background technique [0002] The yellow catfish belongs to the Siluriformes in classification, the family of the family, and the genus of the yellow catfish. In this genus, there are mainly yellow catfish, the yellow catfish (Jianghuang catfish), the fork-tailed yellow catfish and the glossy yellow catfish. 4 types of fish. Yellow catfish is a very important economic fish in freshwater aquaculture in my country. It has strong adaptability to the ecological environment and is widely distributed in freshwater water bodies in my country. In 2014, the aquaculture output reached 340,000 tons. Because of its tender meat, rich nutrition, high meat content, and no intermuscular spines except spines, it is very popular among consumers. [0003] Wache...

Claims

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Application Information

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IPC IPC(8): C07K14/46C12N15/12C12N15/11C12Q1/68
CPCC07K14/461C12Q1/6888
Inventor 梅洁张晋巩高瑞丹成桂建芳
Owner HUAZHONG AGRI UNIV
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