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Sex identification method for juvenile fish of hybrid pelteobagrus fulvidraco (richardson)

A technology for juvenile sex and yellow catfish, which is applied to fish sex identification, quickly and accurately distinguishes the sex of hybrid yellow catfish [Yellow catfish╳Washer's yellow catfish] juveniles, common yellow catfish and "Quanxiong No. 1" "The field of gender identification of juvenile yellow catfish can solve the problems of high equipment requirements, high detection costs, and cumbersome method steps, and achieve the effect of increasing benefits

Inactive Publication Date: 2014-01-22
NANJING NORMAL UNIVERSITY
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Problems solved by technology

[0008] The purpose of the present invention is to provide a method for quickly and accurately identifying the sex of hybrid yellow catfish [Yellow catfish (♀)╳Wachter's yellow catfish (♂)] juvenile fish, and is suitable for common yellow catfish and "Quanxiong No. 1" "The gender identification of juvenile yellow catfish is suitable for production practice, so as to overcome the defects of the existing technology of using modern molecular biology techniques to identify the sex of young yellow catfish, such as high equipment requirements, high detection costs, and cumbersome method steps, etc., The identification method of the present invention is more efficient, economical, fast, accurate and stable, and is suitable for production practice

Method used

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  • Sex identification method for juvenile fish of hybrid pelteobagrus fulvidraco (richardson)
  • Sex identification method for juvenile fish of hybrid pelteobagrus fulvidraco (richardson)
  • Sex identification method for juvenile fish of hybrid pelteobagrus fulvidraco (richardson)

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[0022] 1. Preliminary preparation for identification

[0023] 1) Drugs: 5% acetic acid solution, acetic acid (glacial acetic acid), magenta stain (phenol red), crystal violet stain, ammonium oxalate, 95% ethanol, double distilled water

[0024] 2) Equipment: biological microscope, 1mL syringe, ophthalmic scissors, iris tweezers, glass slide, cover glass, dropper

[0025] 3) Preparation of staining solution: Prepare glacial acetic acid carmine solution and ethanol crystal violet solution according to the method introduced in the manual

[0026] 2. Gender identification:

[0027] The Laboratory of Aquatic Economic Animal Germplasm Resources and Genetic Breeding, School of Life Sciences, Nanjing Normal University, Jiangsu Province, artificially bred hybrid yellow catfish in the Lukou Yellow Catfish Experimental Base in June 2013. Fish (♂)] 100,000 tails, 20,000 ordinary yellow catfish, and 50,000 "Quanxiong No. 1" yellow catfish. 30 days after the opening of the hybrid yellow ...

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Abstract

The invention discloses a sex identification method for juvenile fish of hybrid pelteobagrus fulvidraco (richardson) [pelteobagrus fulvidraco (richardson) (male)* pelteobagrus vachelli (richardson) (female)]. The hybrid pelteobagrus fulvidraco (richardson) having an individual body length of above 3 cm is identified by utilization of a method of living-body gonad squashing, acetocarmine staining and ethanol crystal violet staining, and the accuracy of the method is verified by a paraffin section of the gonad tissue. The sex identification method is suitable for sex identification for juvenile fish of common pelteobagrus fulvidraco (richardson) and of "all-male No.1" pelteobagrus fulvidraco (richardson). By the sex identification method, sex identification of one juvenile fish can be completed in 3 min, and 100 fishes can be identified by cooperation of two persons for 2 h. The sex identification method has characteristics of high efficiency, economy, rapidness, accuracy and stability. The sex identification method has important application value for male fry cultivation of the pelteobagrus fulvidraco (richardson) and the hybrid pelteobagrus fulvidraco (richardson) thereof, and can be applied for rapid sex identification of a pelteobagrus fulvidraco (richardson) commodity summer fry.

Description

technical field [0001] The invention belongs to fish gender identification technology in the technical field of aquatic organisms. More specifically, the invention relates to a method for quickly and accurately identifying hybrid yellow catfish [yellow catfish (♀)╳Vascher's yellow catfish (♂)] juveniles This method is also applicable to the sex identification of common yellow catfish and "Quanxiong No. 1" yellow catfish juveniles. The invention has important application value in cultivating male seedlings of the yellow catfish and its hybrid yellow catfish, and can be applied to the rapid sex identification of commercial yellow catfish seedlings. Background technique [0002] At present, there are mainly two species of yellow catfish cultured, namely, hybrid yellow catfish [yellow catfish (♀)╳Washer's yellow catfish (♂)] and "Quanxiong No. 1" yellow catfish. [0003] Both pelteobagrus fulvidraco (Richardson) and Pelteobagrus vachelli (Richardson) belong to Siluriformes, Bag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N1/30
Inventor 尹绍武张国松汪亚媛王小鲁胡亚丽
Owner NANJING NORMAL UNIVERSITY
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