SRAP molecular marker, kit and method for identifying Pelteobagrus fulvidraco, Pelteobagrus vachelli and first filial generation and application

Abstract

The invention relates to an SRAP molecular marker, a kit and an identification method for identifying Pelteobagrus fulvidraco, Pelteobagrus vachelli and first filial generation of the Pelteobagrus fulvidraco and the Pelteobagrus vachelli and application, and belongs to the technical field of hybrid variety breeding of aquaculture. Amplification primers of the SRAP molecular marker are a forward primer Me3 with a nucleotide sequence as shown in SEQ ID NO.1 and a reverse primer Em3 with a nucleotide sequence as shown in SEQ ID NO.2. The molecular marker provided by the invention can be used forsimply and accurately distinguishing the Pelteobagrus fulvidraco, the Pelteobagrus vachelli and the first filial generation, so that the parent breeding and fry breeding effects are improved.

Description

technical field [0001] The invention relates to the technical field of aquaculture hybrid breed selection, in particular to an SRAP molecular marker, a kit, an identification method and an application for identifying Jianghuang catfish, Washi's yellow catfish and their first generation of hybrids. Background technique [0002] Yellow catfish is one of the important famous and characteristic aquaculture species in my country. Its meat is tender and has no intermuscular spines. It is delicious, nutritious, nourishing and medicinal, so it is deeply loved by consumers. There is a great demand in the market. With the continuous promotion of new breeding technologies and increasingly diverse farming methods, the output of yellow catfish in my country has increased significantly. According to the statistics of "China Fishery Yearbook", it has reached 480,000 tons in 2018, with a rate of about 20% for several consecutive years growing. Among them, Jiangsu, Zhejiang, Hubei, Jiangxi, ...

AI Technical Summary

Problems solved by technology

Usually, it is difficult to distinguish Jianghuang catfish, Washi's yellow catfish and their hybrid offsprings at the seedling stage. They need to be distinguished by their shape and body color when they grow to adult fish. Seedlings of different species are often mixed together. Affect the breeding effect

At present, there is a lack of an effective method for distinguishing the yellow catfish, the yellow catfish and their hybrid offspring at the seedling stage.

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