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Pelteobagrus fulvidraco feed additive, pelteobagrus fulvidraco feed and preparation method of pelteobagrus fulvidraco feed

A feed additive, yellow catfish technology, applied in the molding or processing of animal feed, animal feed, animal feed and other directions, can solve the problems of drug resistance, increase the cost of breeding treatment dose, achieve enhanced effect and reduce antibiotic pollution problem, effect of good effect

Pending Publication Date: 2020-11-10
武汉大北农水产科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the non-standard use of antibiotics causing obvious drug resistance problems, the continuous increase in treatment doses is unacceptable for the breeding environment and breeding costs

Method used

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  • Pelteobagrus fulvidraco feed additive, pelteobagrus fulvidraco feed and preparation method of pelteobagrus fulvidraco feed

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0044] Preparation Example 1: Recovery of Lactobacillus plantarum and Preparation of Seed Solution

[0045] 1. The Lactobacillus plantarum strain tube is opened

[0046] 1) Wipe the strain tube with absorbent cotton containing 75% alcohol;

[0047] 2) Use tweezers to heat the top of the strain tube on the flame of an alcohol lamp to avoid direct heating of the bacteria or excessive heating;

[0048] 3) Use a pipette tip to add a few drops of sterile water to the top of the heated strain tube to crack the glass of the strain tube;

[0049] 4) Knock off the top of the cracked strain tube with tweezers;

[0050] Among them, the freeze-dried powder of Lactobacillus plantarum in the strain tube was purchased from China General Microorganism Strain Collection and Management Center.

[0051] 2. Activation of Lactobacillus plantarum

[0052] 1) Take 0.3mL of sterile MRS liquid medium with a sterile pipette tip, drop it into the strain tube, mix well, and dissolve the freeze-dried ba...

preparation example 2

[0062] Preparation Example 2: Bacillus subtilis recovery and preparation of seed solution

[0063] 1. Open the Bacillus subtilis strain tube

[0064] 1) Wipe the strain tube with absorbent cotton containing 75% alcohol;

[0065] 2) Use tweezers to heat the top of the strain tube on the flame of an alcohol lamp to avoid direct heating of the bacteria or excessive heating;

[0066] 3) Use a pipette tip to add a few drops of sterile water to the top of the heated strain tube to crack the glass of the strain tube;

[0067] 4) Knock off the top of the cracked strain tube with tweezers;

[0068] Among them, the freeze-dried powder of Bacillus subtilis in the strain tube was purchased from China General Microorganism Strain Collection and Management Center.

[0069] 2. Activation of Bacillus subtilis

[0070] 1) Take 0.3mL of sterile LB liquid medium with a sterile pipette tip, drop it into the strain tube, and mix well to dissolve the freeze-dried bacteria into a suspended liqui...

preparation example 3

[0080] Preparation Example 3: Recovery of Aeromonas hydrophila and cultivation of seed solution

[0081] 1. The Aeromonas hydrophila strain tube is opened

[0082] 1) Wipe the strain tube with absorbent cotton containing 75% alcohol;

[0083] 2) Use tweezers to heat the top of the strain tube on the flame of an alcohol lamp to avoid direct heating of the bacteria or excessive heating;

[0084] 3) Use a pipette tip to add a few drops of sterile water to the top of the heated strain tube to crack the glass of the strain tube;

[0085] 4) Knock off the top of the cracked strain tube with tweezers;

[0086] Among them, the freeze-dried powder of Aeromonas hydrophila in the strain tube was purchased from China General Microorganism Strain Collection and Management Center.

[0087] 2. Activation of Aeromonas hydrophila

[0088] 1) Take 0.3mL of sterile LB liquid medium with a sterile pipette tip, drop it into the strain tube, mix well, and dissolve the freeze-dried bacteria into...

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Abstract

The invention relates to a pelteobagrus fulvidraco feed additive, a pelteobagrus fulvidraco feed and a preparation method of the pelteobagrus fulvidraco feed, and relates to the field of aquatic feeds. The pelteobagrus fulvidraco feed additive comprises at least two of a compound Chinese herbal medicine extract, a lactobacillus plantarum seed solution and a bacillus subtilis seed solution. The pelteobagrus fulvidraco feed is prepared from the following raw material substances in percentage by weight: 32-34% of soybean meal, 18-20% of wheat flour, 3.5-4.2% of soybean oil, 1.8-2.2% of monocalcium phosphate, 0.25-0.32% of a mineral trace element additive, 0.23-0.26% of composite vitamins, 0.18-0.21% of choline chloride, 0.08-0.11% of a mildew inhibitor, 0.045-0.052% of an antioxidant, 0.01-1.01% of a pelteobagrus fulvidraco feed additive and the balance of fish meal. The preparation method of the pelteobagrus fulvidraco feed comprises the following steps of S1, solid material cleaning, S2, primary crushing, S3, primary blending, S4, secondary crushing, S5, secondary blending and S6, blending processing. The feed has the advantages of being free of antibiotics, remarkable in antibacterial and disease-resistant effects and balanced in nutrition.

Description

technical field [0001] The invention relates to the field of aquatic feed, in particular to a feed additive for yellow catfish, feed for yellow catfish and a preparation method thereof. Background technique [0002] Yellow catfish is a common freshwater fish belonging to the family Pleurodendronidae and the genus Yellow catfish. Because of its delicious meat and intermuscular spines, it is very popular among consumers, and the aquaculture output is also increasing year by year. However, in recent years, diseases in the cultivation of yellow catfish have occurred frequently, especially pathogenic fungal diseases, which have caused huge economic losses and waste of resources to the cultivation. Aeromonas hydrophila is a common opportunistic pathogen in aquaculture, belonging to Gram-negative short bacillus, mesophilic. Skin ulcers and acute hemorrhagic enteritis will appear in yellow catfish infected with Aeromonas hydrophila, which are relatively common diseases in the breed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/30A23K10/18A23K10/37A23K20/158A23K20/26A23K20/174A23K20/10A23K10/22A23K20/20A23K40/10A61K36/889A61P31/04A61P37/04A61K35/742A61K35/747
CPCA23K50/80A23K10/30A23K10/18A23K10/37A23K20/158A23K20/26A23K20/174A23K20/10A23K10/22A23K20/30A23K40/10A61K36/734A61K36/752A61K36/575A61K36/708A61K36/634A61K36/889A61K36/718A61K36/185A61K36/484A61K36/30A61P31/04A61P37/04A61K35/747A61K35/742A23V2400/169A61K2300/00Y02A40/818Y02P60/87
Inventor 周建成邹琦蒋勇成琳潘望城杨传哲金柏涛
Owner 武汉大北农水产科技有限公司
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