39 results about "Parabiosis" patented technology

The present invention relates to a combination of a Neotyphodium species endophyte or endophyte culture in a symbiotic association with a host grass which association does not cause symptoms of toxicosis in animals through exclusion of ergovaline but, due to the inclusion of alkaloids including agroclavine, setoclavine or isosetoclavine, retains the ability to resist abiotic stresses and protect the grass from pests.

The object of the invention are compositions and medical treatment methods with an inheritable, Gram-negative, strictly anaerobic and commensal bacterium of the family Christensenellaceae belonging to an OTU (Operational Taxonomic Unit) characterized by a 16S rRNA sequence SEQ ID NO: 1 or to an OTU characterized by a 16S rRNA sequence SEQ ID NO: 2.

The invention provides gut commensal bacteria that have been modified to express one or more biologically active polypeptides or protiens, the bacteria includes a promoter, such as a xylanase promoter, which is induced in response to the presence of xylan in the diet and which regulates the expression of the biologically active polypeptide or protien.

The present disclosure describes compositions and methods for increasing the abundance of commensal bacteria belonging to the order Clostridiales, including Blautia, Ruminococcus, Clostridium, Eubacterium, Holdemania and Dorea species, that are associated with reduced lethal GVHD and improved overall survival following bone marrow or hematopoietic stem cell transplant. The present disclosure, therefore, provides methods for reducing the likelihood, incidence or severity of GVHD by (1) avoiding the loss of endogenous beneficial species through antibiotic selection; (2) by administering a therapeutically effective amount of a composition comprising one or more Clostridiales associated with reduced GVHD to individuals who may lack or have lost those strains from their intestinal microbiota. Additionally, support for endogenous or reestablished Clostridiales related to reduced GVHD as a treatment option for reducing GVHD can also be provided in the form of nutritional supplementation, for example, sugars fermented by some species of Clostridiales with GVHD reducing activity.

The invention relates to a bacterial solution of intestinal florae of healthy people, and a preparation method and quality control of the bacterial liquid. The invention also relates to an applicationof combination of the bacterial solution of intestinal florae of healthy people and an immune signal pathway modulator in treating tumor patients to improve response of tumors to immunotherapy.

The invention discloses a composition for delaying senility. The composition is prepared from the following components in parts by weight: 4 to 30 parts of collagen, 0.02 to 2 parts of haematococcus pluvialis, 0.01 to 0.5 part of tremella polysaccharides, 0.01 to 1 part of coniferous cherry powder, 0.1 to 2 parts of vitamin C, 3 to 10 parts of ingredients and 50 to 98 parts of purified water. According to the composition for delaying the senility, disclosed by the invention, all the components are synthesized, so that exogenous water-preserving antioxidant substances required by a human body can be supplemented and endogenous water-preserving antioxidant substances in the human body also can be increased; the interaction of exogenous and endogenous antioxidant substances is promoted to form a water-preserving antioxidant symbiotic ring, so that a comprehensive and effective synthetic effect in water-replenishing and anti-oxidation is produced; the composition is safe and reliable and has no toxic and side effects. A method for effectively detecting the content of the components in the composition is adopted, and especially the method for detecting the content of astaxanthin in a solution containing a protein sample has the advantages of simple operation, high sensitivity and wide application.

The invention provides a primer compound for identifying intestinal microecological state and an application thereof. The primer compound is a specific primer designed for intestinal bacterium 16S rRNA; the specific primer includes random basic groups; the quantity of random basic groups is 3-5; the intestinal bacterium includes any one of or combination of at least two of symbiotic clostridia, bifidobacterium adolescentis, fusobacterium nucleatum, peptostreptococcus anaerobius or klebsiella. A kit researched and developed on the basis of the primer compound provided by the invention has a specificity for detecting intestinal microecological imbalance reaching up to 92.5% and a sensitivity reaching up to 80.43%.

The invention provides an HNC (Head and Neck Cancer) prognosis biomarker based on a lymph node microbial flora and application of the HNC prognosis biomarker, and belongs to the technical field of disease prognosis and molecular biology. According to the invention, the 16S ribosomal RNA gene of the lymph node microbial flora of an HNC patient is subjected to high-throughput sequencing, so that it is proved that different species exist in flora species of metastatic lymph nodes and non-metastatic lymph nodes in the HNC patient; furthermore, through a microbial flora symbiotic relationship network, it is found that the flora interrelation between the species of the metastatic lymph node and the species of the non-metastatic lymph node of the HNC patient is also different at the genus level; the result discovers the characteristics of the microbiome of the metastatic lymph node and the non-metastatic lymph node of the HNC patient for the first time; and meanwhile, it is known by analysis that the flora difference characteristics (diversity index values and relative abundance of different species) have a good prediction effect on the lifetime (total lifetime and three-year lifetime) of the HNC patient, so that the invention has a good practical application value.

The invention discloses a kit for identifying colorectal cancer. The kit is characterized by comprising a specific primer combination for identifying indicating bacteria, wherein the primer combination is shown as SEQ ID NO.1-12, and the indicating bacteria are a combination of F. nucleatum, P. anaerobius, C. symbiosum, E. faecalis, P. asaccharolytica and S. salivaricus. The invention also discloses an application of the kit. The kit has the beneficial effects that risk prediction of colorectal cancer can be carried out only by collecting stool samples. The principle is to detect colorectal cancer pathogenic bacteria in feces, wherein the risk assessment sensitivity can reach 92% and the specificity is 95%.

The invention relates to a group of bemisia tabaci prevention and control target genes and combined application thereof. The group of bemisia tabaci prevention and control target genes comprise a BtGlu gene as shown in SEQ ID NO.1, a BtArgH gene as shown in SEQ ID NO.2 and a BtTY gene as shown in SEQ ID NO.3. The research thought of taking a sugar transporter gene, a basic group and a amino acid synthetic gene as the bemisia tabaci prevention and control target genes is provided for the first time; and experiments of the survival rate of the adult bemisia tabaci, the egg laying rate of the female bemisia tabaci, the influence on symbiotic bacteria of the female bemisia tabaci, the influence on the virus acquiring capacity of the female bemisia tabaci TYLCV and the like verify that the silent expression can obtain a short-term prevention and control effect-reduction of the survival rate of the bemisia tabaci and a long-term prevention and control effect-reduction of the reproductive capacity of the bemisia tabaci.

The invention discloses dsRNA of a gene of a symbiotic bacterium of aphids and an application thereof to inhibition of growth of aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids is obtained; the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids can be inhibited or silenced, impaired growth and development of the wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.

The invention belongs to the technical field of bioinformatics research of medicinal plants, and particularly relates to a bioinformatics analysis and identification method of a V3 region and a V4 region of a microorganism 16S rRNA gene based on high-throughput sequencing. The bioinformatics analysis and identification method includes the following steps that a sample is collected, and pre-processing operation is carried out; a high-throughput sequencing method is used for obtaining double-strand sequencing data of sequences of the V3 region and the V4 region of the microorganism 16S rRNA genein the sample; qualified data is selected with the aid of FastQC software; quality control processing is carried out on a total chain file with Mothur software to obtain a quality controlled file; the quality controlled file is analyzed with the aid of Qiime software; a visual result analysis chart is drawn with the aid of R software and LEfSe software; and results are compared to identify samplemicroorganisms. The sample microorganisms can be quickly identified, so that the cultivation methods of traditional Chinese medicinal materials can be identified and the specific action mechanism oftradition Chinese medicinal materials can be explored, the possibility is provided to maximize using of the efficacy of traditional Chinese medicinal materials, and the characteristics of short identification cycle and high result accuracy are achieved.

The object of the invention are compositions and medical treatment methods with an inheritable, Gram-negative, strictly anaerobic and commensal bacterium of the family Christensenellaceae belonging to an OTU (Operational Taxonomic Unit) characterized by a 16S rRNA sequence SEQ ID NO: 1 or to an OTU characterized by a165 rRNA sequence SEQ ID NO: 2.

The present invention relates to a selection of probiotic strains belonging to the genus Bifidobacterium and to a symbiotic composition containing the same, intended for feeding infants.

The invention discloses a breeding method for preventing gastrodia tuber from degradation, which comprises the following steps: detecting gastrodia tuber by simultaneously using AFLP or ISSR, SSR and gastrodine content markers, and drawing a clustering graph and a gastrodia tuber genetic polymorphism / gastrodine content relational graph by using software, thereby quickly analyzing the gastrodia tuber germplasm resource kinship, identifying the gastrodia tuber inbred line and hybrid, quickly selecting and determining parents of the gastrodia tuber, quickly identifying the purity of the gastrodia tuber hybrid, etc., so that the traditional Chinese medicine gastrodia tuber has high quality and is sustainable. The method disclosed by the invention can scientifically, quickly, effectively and progressively solving the problem in the gastrodia tuber seed source, and can select high-quality traditional Chinese medicine gastrodia tuber seeds applicable to local cultivation; under the conditions suitable for intergrowth of germination bacteria and Armillaria mellea as well as scientific management, the yield and quality of the gastrodia tuber seeds are high; and after three generations of reproduction, the content of gastrodine still conforms to the specification in pharmacopoeia.

The invention discloses application of phosphorylated bacterioprotein NopL. According to the invention, NopL and a mutant thereof are successfully expressed and purified in vitro; a phosphorylation experiment is carried out, and it is discovered that NopL can be directly phosphorylated by SIPK, wherein NopL is the first discovered bacterioprotein which can be directly used as an SIPK substrate. Moreover, through construction of the mutant of a NopL-deleted phosphorylation site, it is found that NopL loses its original promotion effect on symbiosis of leguminous plants when NopL cannot be phosphorylated by protein kinase, so it is proved that the symbiosis promotion function of NopL is depended on phosphorylation. Such a discovery provides a novel direction for further research on the interaction mechanism of leguminous plants and rhizobium and has high theoretical and practical values.

The invention discloses a mild bacteriophage VneM1 for regulating and controlling coral flora and application of the mild bacteriophage VneM1. The preservation number of the bacteriophage is Vibrio neocaledonicus bacteroiphage VneM1 is CGMCC NO.61215-B1. The invention also discloses a preparation method of the bacteriophage. According to the invention, the new bacteriophage-Vibrio neocaledonicus bacteriophage VneM1 is separated from a coral symbiotic bacterium Vibrio neocaledonicus SCSIO 43009. The bacteriophage VneM1 has a cracking effect on coral symbiotic vibrio, and regulates and controlsthe composition of coral symbiotic microorganisms. Hydranth infection experiments prove that the bacteriophage VneM1 can delay coral whitening caused by temperature rise. The bacteriophage VneM1 is amild bacteriophage, and under normal conditions, the bacteriophage VneM1 is integrated on an original lysozyme SCSIO 43009 genome and exists in a form of a prophage. The bacteriophage can be producedby spontaneous lysogen-lysis conversion, and the lysogen-lysis conversion can be promoted by environmental factors such as SOS stress reaction.

The present invention provides compositions comprising populations of commensal bacteria isolated from a microbiome sample of a mammalian subject and engineered to express a heterologous polynucleotide, compositions comprising such engineered commensal bacteria and methods of use for delivering a therapeutic polypeptide to a mammal, e.g, by administering the engineered commensal / native bacteria.

The invention provides a polypeptide for inhibiting dietary obesity. The amino acid sequence of the polypeptide is as follows: X1TX2YX3RTGR. The invention also provides application of the polypeptide. According to the polypeptide disclosed by the invention, the weight growth rate of experimental animals is reduced, subcutaneous and visceral fat deposition is reduced, occurrence and development of dietary obesity of experimental animals such as mice, rats and rhesus monkeys can be obviously inhibited by reducing appetite, and the abundance of intestinal symbiotic bacteria Akecmania of the experimental animals treated by the polypeptide is obviously improved.

The invention discloses a photobioreactor for improving ganoderma lucidum triterpenoids and a construction method of a phycomycete symbiotic system. The photobioreactor is internally provided with a barrier film; and the barrier film divides the photobioreactor into a first incubator and a second incubator, the first incubator is used for culturing chlorella pyrenoidosa, the second incubator is used for culturing chlorella pyrenoidosa, and the barrier film is used for preventing a chlorella pyrenoidosa cell body and a ganoderma lucidum cell body from being adhered and adhered. The construction method of the phycomycete symbiotic system by adopting the photobioreactor comprises the steps: S1, inoculating chlorella pyrenoidosa cells into a PDB culture medium in the first incubator; S2, inoculating the ganoderma lucidum cells into a PDB culture medium in the second incubator; and S3, placing the photobioreactor inoculated with the chlorella pyrenoidosa bacteria and the ganoderma lucidum bacteria cells in a constant-temperature and constant-light sterile room, setting the temperature to be 28-30 DEG C, setting the illumination intensity to be 130 [mu]mol / m<2> / s-150 [mu]mol / m<2> / s, and culturing for 10-15 days. The phycomycete symbiotic system disclosed by the invention has a remarkable effect of improving the ganoderma lucidum triterpenoids.

The combination of specific immunoglobulins plus activated Bifidobacteria strains or other beneficial bacteria is described with the designed efficacy to colonize unstable microbiome communities in humans or other animals, restoring the keystone Bifidobacteria strains or other beneficial bacteria to compositional and functional importance in the intestine and improve overall health and reduce pathogenic infections in the host. Secretory immunoglobulin A (SIgA), when bound via specific glycans to select commensal bacteria grown on human milk oligosaccharides (HMOs), enhances the colonization potential of commensals through protection from intestinal digestion, enhancing attachment, and dampening host immune response.

The invention discloses a method for rapidly detecting symbiotic bacteria in intestinal tracts / fat bodies of brown planthoppers, and aims to overcome the defects of the conventional method for quantitatively detecting the symbiotic bacteria of the brown planthoppers. According to the invention, plasmids containing an Escherichia coli 16S rDNA V3V4 region and a brown planthopper actin betaactin sequence are used for respectively constructing standard curves, a universal fluorescent quantitative PCR reaction detection system is established, and the total number of symbiotic bacteria in the intestinal tract or fat body of the brown planthopper in a unit insect body can be detected.

The invention discloses silver nano hydrogel for regulating flora to activate immune response and a preparation method and application thereof, and belongs to the field of biological medicine. The silver nano hydrogel consists of silver nanoparticles and mucous membrane adhesion gel. The silver nano hydrogel contains inorganic silver nanoparticles, natural high-molecular polysaccharide, a polyaldehyde polymer material and a deacetylation polymer material. The silver nano hydrogel can adjust flora abundance of mucous membranes, can inhibit proliferation of other bacteria, and meanwhile, can promote proliferation of immunologically activated bacteria such as anaerobic streptococcus and the like, so that immune response is enhanced. Meanwhile, the gel can also be adhered to human mucous membranes, so that long-time retention at affected parts is implemented, and the human symbiotic flora is continuously regulated. The silver nano hydrogel is biodegradable, excellent in biocompatibility, safe and free of toxic and side effects, and has the advantages of simplicity and convenience for preparation, continuity, high efficiency and the like. The silver nano hydrogel has important social and economic significance in the field of biomedicine of regulating the symbiotic flora, activating human immunity, assisting cancer resistance and the like.

The invention provides an Armillaria cepistipes Velen. strain and a method for preparing the same. A systematic name of the strain is Armillaria cepistipes Velen., and a preservation registration number of the strain is CGMCC No.11902. The method includes strain selection, separation and purification, identification and cultivation. The Armillaria cepistipes Velen. strain and the method have the advantages that risks of low gastrodia elata Bl, yield or zero-output 'empty pond' phenomena due to inappropriate strain selective breeding can be reduced; the strain and gastrodia elata BL. f. glauca S. Chow are good in affinity, and accordingly the original quality of the gastrodia elata BL. f. glauca S. Chow in main gastrodia elata BL. f. glauca S. Chow producing regions under large-scale artificial cultivation conditions can be guaranteed.

The invention provides a method for detecting a low-virulence infection in a patient, by distinguishing true infections from false positives. The method comprises testing for the presence or amount of commensal microorganism(s) in a patient sample, and in particular, the commensal microorganism is one that may be causative of a low-virulence infection. Since testing for commensal microorganisms will lead to a large number of false-positives due to frequent sample contamination, the sample is also tested to discriminate true chronic infection from these false positives. In accordance with the invention, false positives are discriminated by evaluating a nucleic acid profile from the sample.