39 results about "Parabiosis" patented technology

The invention provides an HNC (Head and Neck Cancer) prognosis biomarker based on a lymph node microbial flora and application of the HNC prognosis biomarker, and belongs to the technical field of disease prognosis and molecular biology. According to the invention, the 16S ribosomal RNA gene of the lymph node microbial flora of an HNC patient is subjected to high-throughput sequencing, so that it is proved that different species exist in flora species of metastatic lymph nodes and non-metastatic lymph nodes in the HNC patient; furthermore, through a microbial flora symbiotic relationship network, it is found that the flora interrelation between the species of the metastatic lymph node and the species of the non-metastatic lymph node of the HNC patient is also different at the genus level; the result discovers the characteristics of the microbiome of the metastatic lymph node and the non-metastatic lymph node of the HNC patient for the first time; and meanwhile, it is known by analysis that the flora difference characteristics (diversity index values and relative abundance of different species) have a good prediction effect on the lifetime (total lifetime and three-year lifetime) of the HNC patient, so that the invention has a good practical application value.

The invention provides gut commensal bacteria that have been modified to express one or more biologically active polypeptides or protiens, the bacteria includes a promoter, such as a xylanase promoter, which is induced in response to the presence of xylan in the diet and which regulates the expression of the biologically active polypeptide or protien.

The invention belongs to the technical field of bioinformatics research of medicinal plants, and particularly relates to a bioinformatics analysis and identification method of a V3 region and a V4 region of a microorganism 16S rRNA gene based on high-throughput sequencing. The bioinformatics analysis and identification method includes the following steps that a sample is collected, and pre-processing operation is carried out; a high-throughput sequencing method is used for obtaining double-strand sequencing data of sequences of the V3 region and the V4 region of the microorganism 16S rRNA genein the sample; qualified data is selected with the aid of FastQC software; quality control processing is carried out on a total chain file with Mothur software to obtain a quality controlled file; the quality controlled file is analyzed with the aid of Qiime software; a visual result analysis chart is drawn with the aid of R software and LEfSe software; and results are compared to identify samplemicroorganisms. The sample microorganisms can be quickly identified, so that the cultivation methods of traditional Chinese medicinal materials can be identified and the specific action mechanism oftradition Chinese medicinal materials can be explored, the possibility is provided to maximize using of the efficacy of traditional Chinese medicinal materials, and the characteristics of short identification cycle and high result accuracy are achieved.

The present invention relates to a combination of a Neotyphodium species endophyte or endophyte culture in a symbiotic association with a host grass which association does not cause symptoms of toxicosis in animals through exclusion of ergovaline but, due to the inclusion of alkaloids including agroclavine, setoclavine or isosetoclavine, retains the ability to resist abiotic stresses and protect the grass from pests.

The invention discloses application of phosphorylated bacterioprotein NopL. According to the invention, NopL and a mutant thereof are successfully expressed and purified in vitro; a phosphorylation experiment is carried out, and it is discovered that NopL can be directly phosphorylated by SIPK, wherein NopL is the first discovered bacterioprotein which can be directly used as an SIPK substrate. Moreover, through construction of the mutant of a NopL-deleted phosphorylation site, it is found that NopL loses its original promotion effect on symbiosis of leguminous plants when NopL cannot be phosphorylated by protein kinase, so it is proved that the symbiosis promotion function of NopL is depended on phosphorylation. Such a discovery provides a novel direction for further research on the interaction mechanism of leguminous plants and rhizobium and has high theoretical and practical values.

Methods and compositions for controlling termite populations by leveraging newly discovered mechanisms for symbiont- and host mediated anti-fungal defenses in the termite hind-gut. Such methods may include administering a composition to a termite, the composition formulated to promote protist dysbiosis in the gut of the termite, or silencing specific host or symbiont genes by RNA interference, and exposing the termite to a pathogen, where the composition increases the susceptibility of the termite to the pathogen. Compositions are also provided for the treatment of fungal infections, the composition comprising a medically effective amount of recombinant glycosyl hydrolase family 7 cellulase, as well as methods for utilizing the same.

The invention discloses the application of sulfadiazine to the reproductive behavior of Trichogramma brevis infected with Wolbachia. The invention provides the application of sulfadiazine in eliminating symbiotic Wolbachia or reducing the titer of Wolbachia in animals. The present invention finds for the first time that the honey water containing 1.0mg / ml sulfadiazine alone is used to feed the short-tube Trichogramma, which can well remove the Wolbachia carried by the short-tube Trichogramma. This method is more convenient, time-saving, and obtains complete male offspring. This will lay the foundation for the further use of Wolbachia to regulate the reproductive mode of arthropods, and has good application value and promotion significance.

The invention provides an Armillaria cepistipes Velen. strain and a method for preparing the same. A systematic name of the strain is Armillaria cepistipes Velen., and a preservation registration number of the strain is CGMCC No.11902. The method includes strain selection, separation and purification, identification and cultivation. The Armillaria cepistipes Velen. strain and the method have the advantages that risks of low gastrodia elata Bl, yield or zero-output 'empty pond' phenomena due to inappropriate strain selective breeding can be reduced; the strain and gastrodia elata BL. f. glauca S. Chow are good in affinity, and accordingly the original quality of the gastrodia elata BL. f. glauca S. Chow in main gastrodia elata BL. f. glauca S. Chow producing regions under large-scale artificial cultivation conditions can be guaranteed.

The invention discloses a symbiotic fungus Aspergillus fumigatus, a preparation method, extracts and applications thereof. The symbiotic fungus Aspergillus fumigatus ( Aspergillus fumigatus ) The HX-1 strain was deposited in the China Center for Type Culture Collection on June 15, 2018, and the preservation address is Luojia Mountain, Wuchang, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M 2018377. ( Vibrio anguillarum ), Vibrio harveii ( Vibrio harvey ), Vibrio parahaemolyticus ( Vibrio parahaemolyticus ), Vibrio cholerae ( Vibrio cholerae ), Aeromonas hydrophila ( Aeromonas hydrophila ) and Shewanella spoilage ( Shewanella putrefaciens ) has an inhibitory effect, therefore, the realization of the present invention provides candidate drugs for the research and development of new antibacterial drugs.