Identification method of symbiotic microorganisms of medicinal plants and application of identification method

Abstract

The invention belongs to the technical field of bioinformatics research of medicinal plants, and particularly relates to a bioinformatics analysis and identification method of a V3 region and a V4 region of a microorganism 16S rRNA gene based on high-throughput sequencing. The bioinformatics analysis and identification method includes the following steps that a sample is collected, and pre-processing operation is carried out; a high-throughput sequencing method is used for obtaining double-strand sequencing data of sequences of the V3 region and the V4 region of the microorganism 16S rRNA genein the sample; qualified data is selected with the aid of FastQC software; quality control processing is carried out on a total chain file with Mothur software to obtain a quality controlled file; the quality controlled file is analyzed with the aid of Qiime software; a visual result analysis chart is drawn with the aid of R software and LEfSe software; and results are compared to identify samplemicroorganisms. The sample microorganisms can be quickly identified, so that the cultivation methods of traditional Chinese medicinal materials can be identified and the specific action mechanism oftradition Chinese medicinal materials can be explored, the possibility is provided to maximize using of the efficacy of traditional Chinese medicinal materials, and the characteristics of short identification cycle and high result accuracy are achieved.

Description

technical field [0001] The invention belongs to the technical field of bioinformatics research, in particular to a method for identifying medicinal plant symbiotic microorganisms based on high-throughput sequencing and its application. Background technique [0002] High-throughput sequencing is also called second-generation sequencing technology, and sequencing platforms such as Roche / 454FLX, Illumina / GenomeAnalyzer / Hiseq / Miseq, and Applied Biosystems SOLID are usually used. Microbial 16S rRNA research method based on high-throughput sequencing technology is currently one of the most effective and important methods for understanding and analyzing the structure and function of microbial hybrids. With the development of "omics" theories such as modern genomics, proteomics, and metabolomics, the introduction of the perspective of systems biology and the application of bioinformatics, through the analysis of species-related RNA and 16S rRNA sequencing data, we can get Informati...

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Problems solved by technology

[0004] However, the existing analysis methods have disadvantages such as slow analysis speed, poor accuracy, and a long cycle for the entire analysis and identification, and because the analysis method has the significance of statistical analysis, the results are not necessarily scientific and practical, etc.

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