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Photobioreactor for improving ganoderma lucidum triterpenoids and construction method of phycomycete symbiotic system

A technology for photobioreactors and triterpenoids, applied in photobioreactors, methods for stress-stimulating microbial growth, bioreactors/fermenters for specific purposes, etc., to achieve the effect of promoting yield and benefiting yield

Pending Publication Date: 2021-06-22
SHANGHAI INST OF TECH
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the improvement of the production of triterpenoids produced by Ganoderma lucidum is still one of the technical bottlenecks in the extraction of this active anticancer substance

Method used

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  • Photobioreactor for improving ganoderma lucidum triterpenoids and construction method of phycomycete symbiotic system
  • Photobioreactor for improving ganoderma lucidum triterpenoids and construction method of phycomycete symbiotic system

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Embodiment 1

[0032] Chlorella pyrenoidosa (purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, No. FACHB-5) and Ganoderma lucidum (purchased from the China General Microorganism Culture Collection Center, No. 5.896) in the logarithmic phase were respectively inoculated in freshly prepared and sterilized In the PDB medium of bacteria, carry out sterile culture, co-cultivate for 3 generations, the growth is stable and the algae cell concentration reaches 1×10 5 Cell / mL is used for the construction of algae-bacteria symbiosis system; 5mL Chlorella pyrenoidosa cells and Ganoderma lucidum were respectively inoculated in the first incubator and the first incubator of the photobioreactor containing 500mL sterilized PDB medium. Second incubator.

[0033] A single type of chlorella pyrenoidosa system, a single type of ganoderma lucidum system, a separate culture system for algae and bacteria, and a mixed culture system for algae and bacteria were set up. Both the f...

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Abstract

The invention discloses a photobioreactor for improving ganoderma lucidum triterpenoids and a construction method of a phycomycete symbiotic system. The photobioreactor is internally provided with a barrier film; and the barrier film divides the photobioreactor into a first incubator and a second incubator, the first incubator is used for culturing chlorella pyrenoidosa, the second incubator is used for culturing chlorella pyrenoidosa, and the barrier film is used for preventing a chlorella pyrenoidosa cell body and a ganoderma lucidum cell body from being adhered and adhered. The construction method of the phycomycete symbiotic system by adopting the photobioreactor comprises the steps: S1, inoculating chlorella pyrenoidosa cells into a PDB culture medium in the first incubator; S2, inoculating the ganoderma lucidum cells into a PDB culture medium in the second incubator; and S3, placing the photobioreactor inoculated with the chlorella pyrenoidosa bacteria and the ganoderma lucidum bacteria cells in a constant-temperature and constant-light sterile room, setting the temperature to be 28-30 DEG C, setting the illumination intensity to be 130 [mu]mol / m<2> / s-150 [mu]mol / m<2> / s, and culturing for 10-15 days. The phycomycete symbiotic system disclosed by the invention has a remarkable effect of improving the ganoderma lucidum triterpenoids.

Description

technical field [0001] The invention belongs to the field of biomedical technology manufacturing, and in particular relates to a photobioreactor for increasing the triterpene compound of Ganoderma lucidum and a method for constructing an algal-bacteria symbiosis system using the photobioreactor to increase the content of the triterpene compound. Background technique [0002] The research and development of anti-tumor drugs has always been a research hotspot in biomedical technology. Natural anti-cancer substances have the great advantage of less toxic and side effects. Triterpenoids are typical active anticancer substances, and Ganoderma lucidum can produce a large amount of triterpenoids, so how to increase the content of triterpenoids in Ganoderma lucidum has become a hot spot of concern. At present, the improvement of the production of triterpenoids produced by Ganoderma lucidum is still one of the technical bottlenecks in the extraction of this active anticancer substanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M1/00C12N1/14C12N1/12C12P39/00C12P33/00C12R1/89C12R1/645
CPCC12M21/02C12M23/34C12M29/04C12M35/08C12N1/14C12N1/12C12P39/00C12P33/00
Inventor 吉喜燕侯梅芳吴世超袁晓磊宋丽莉
Owner SHANGHAI INST OF TECH
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