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Group of bemisia tabaci prevention and control target genes and combined application thereof

A technology of bemisia tabaci and target genes, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of unsatisfactory control effect of bemisia tabaci, endangering ecological environment food safety, and insecticide resistance level of bemisia tabaci Increase and other issues

Active Publication Date: 2021-06-25
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of chemical pesticides not only endangers the rural ecological environment and people's food safety, but also leads to an increase in the level of insecticide resistance in whiteflies
In addition, due to the characteristics of overlapping generations and rapid reproduction of whitefly, traditional insecticides are not ideal for the control of whitefly

Method used

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  • Group of bemisia tabaci prevention and control target genes and combined application thereof
  • Group of bemisia tabaci prevention and control target genes and combined application thereof
  • Group of bemisia tabaci prevention and control target genes and combined application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Effects of Feeding dsRNAs on the Survival Rate of Bemisia tabaci Adults

[0020] 1. Design specific primers for BtGlu gene, BtArgH gene and BtTK gene (see SEQ ID NO.4~SEQ ID NO.9 respectively for its sequence information), and use cDNA as template amplification to obtain double-stranded DNA (abbreviated as BtGlu, BtArgH and BtTK), its sequence information is shown in SEQ ID NO.1 (5'→3'), SEQ ID NO.2 (5'→3'), SEQ ID NO.3 (5'→3');

[0021] 2. Using the double-stranded DNA obtained by the in vitro reverse transcription method as a template, the double-stranded RNA (dsBtGlu, dsBtArgH and dsBtTK for short) was synthesized and purified by in vitro transcription method;

[0022] 3. Mix dsBtGlu, dsBtArgH and dsBtTK in equal amounts (the resulting solution is built into dsRNAs);

[0023] 4. Take a cylindrical double-pass glass tube with an inner diameter of 2 cm and a height of 5 cm, pull the Parafilm membrane to the end of the double-pass glass tube, drop 200 μl of ...

Embodiment 2

[0026] Example 2: Effect of feeding dsRNAs on egg laying rate of Bemisia tabaci females

[0027] 1. Design specific primers for BtGlu gene, BtArgH gene and BtTK gene (see SEQ ID NO.4~SEQ ID NO.9 respectively for its sequence information), and use cDNA as template amplification to obtain double-stranded DNA (abbreviated as BtGlu, BtEAAs and BtPYs), the sequence information of which is shown in SEQ ID NO.1 (5'→3'), SEQ ID NO.2 (5'→3'), SEQ ID NO.3 (5'→3');

[0028] 2. Using the double-stranded DNA obtained by the in vitro reverse transcription method as a template, double-stranded RNAs (dsBtGlu, dsBtEAAs and dsBtPYs for short) were synthesized and purified by in vitro transcription method;

[0029] 3. Mix dsBtGlu, dsBtEAAs and dsBtPYs in equal amounts (the resulting solution is built into dsRNAs);

[0030]4. Take a cylindrical double-pass glass tube with an inner diameter of 2 cm and a height of 5 cm, pull the Parafilm membrane to the end of the double-pass glass tube, drop 200...

Embodiment 3

[0035] Example 3: Effect of Feeding dsRNAs on Symbiotic Bacteria of Bemisia tabaci Females

[0036] 1. Design specific primers for BtGlu gene, BtArgH gene and BtTK gene (see SEQ ID NO.4~SEQ ID NO.9 respectively for its sequence information), and use cDNA as template amplification to obtain double-stranded DNA (abbreviated as BtGlu, BtArgH and BtTK), its sequence information is shown in SEQ ID NO.1 (5'→3'), SEQ ID NO.2(5'→3'), SEQ ID NO.3(5'→3');

[0037] 2. Using the double-stranded DNA obtained by the in vitro reverse transcription method as a template, the double-stranded RNA (dsBtGlu, dsBtArgH and dsBtTK for short) was synthesized and purified by in vitro transcription method;

[0038] 3. Mix dsBtGlu, dsBtArgH and dsBtTK in equal amounts (the resulting solution is built into dsRNAs);

[0039] 4. Take a cylindrical double-pass glass tube with an inner diameter of 2 cm and a height of 5 cm, pull the Parafilm membrane to the end of the double-pass glass tube, drop 200 μl of s...

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Abstract

The invention relates to a group of bemisia tabaci prevention and control target genes and combined application thereof. The group of bemisia tabaci prevention and control target genes comprise a BtGlu gene as shown in SEQ ID NO.1, a BtArgH gene as shown in SEQ ID NO.2 and a BtTY gene as shown in SEQ ID NO.3. The research thought of taking a sugar transporter gene, a basic group and a amino acid synthetic gene as the bemisia tabaci prevention and control target genes is provided for the first time; and experiments of the survival rate of the adult bemisia tabaci, the egg laying rate of the female bemisia tabaci, the influence on symbiotic bacteria of the female bemisia tabaci, the influence on the virus acquiring capacity of the female bemisia tabaci TYLCV and the like verify that the silent expression can obtain a short-term prevention and control effect-reduction of the survival rate of the bemisia tabaci and a long-term prevention and control effect-reduction of the reproductive capacity of the bemisia tabaci.

Description

technical field [0001] The invention relates to the technical field of biological pest control, in particular to a group of control target genes for whitefly control and their combined application. Background technique [0002] Bemisia tabaci belongs to Hemiptera Bemiptera, which is an important pest that harms vegetables, flowers, cotton and other economic crops in my country. By directly feeding, spreading plant viruses and secreting honeydew to induce mildew, Bemisia tabaci reduces the quality and yield of host crops, restricting the development of modern agriculture in China. [0003] At present, field control of Bemisia tabaci mainly relies on chemical control. By using chemical pesticides to reduce the number of field insects, indirectly reduce the harm of whitefly-transmitted viral diseases. However, the extensive use of chemical pesticides not only endangers the rural ecological environment and people's food safety, but also leads to an increase in the level of res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/113C12N15/11A01N57/16A01P7/04
CPCC07K14/43563C12N15/113A01N57/16C12N2310/14
Inventor 杨泽众刘佰明白义川王芳
Owner 天津市农业科学院
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