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Kit for identifying colorectal cancer and application thereof

A colorectal cancer and kit technology, applied in the field of kits for the identification of colorectal cancer, can solve problems such as pain, high physical fitness requirements, incomplete and accurate judgment results, etc., and achieve the effect of high sensitivity

Pending Publication Date: 2020-06-26
南京派森诺基因科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Moreover, in the prior art, the results of fecal occult blood detection are often used to judge whether there is colorectal cancer. However, the judgment results are not completely accurate, and it is often necessary to combine colonoscopy for further examination, and colonoscopy is very important for the examiner himself. High physical requirements, and more painful

Method used

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  • Kit for identifying colorectal cancer and application thereof
  • Kit for identifying colorectal cancer and application thereof
  • Kit for identifying colorectal cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] The accuracy of SVM algorithm (support vector machine) is higher than that of manual interpretation

[0077] The application of detecting the abundance of 9 kinds of intestinal bacteria in feces and the results of fecal occult blood in the risk assessment and diagnosis of colorectal cancer.

[0078] 1 Materials and methods

[0079] 1.1 Sample source

[0080] Two fecal samples from patients with colorectal cancer in the Anorectal Department of Liaoning Cancer Hospital from January 1, 2017 to June 30, 2018 were collected through cooperative relationship, which were marked as sample 1 and sample 2 respectively.

[0081] 1.2 Specimen extraction

[0082] Use the feces collection box, tear off the adhesive, put it in a plastic bag; paste the box on the toilet, and excrete the feces into the feces collection box; take a sample (about 5 spoons of feces) into the feces storage tube with a sampling spoon, and then tighten the tube firmly Cover; put the feces preservation tube ...

Embodiment 2

[0129] Example 2: Determining the optimal combination of indicator bacteria using the stool sample support vector machine method

[0130] 1. Treat the feces

[0131] The specific experimental steps in this example are the same as those in Example 1. The feces of healthy people and patients with colorectal cancer were collected, the bacterial genome was extracted, PCR was performed with 16s rRNA primers, and the values ​​were recorded by the calculation method of the threshold line (Ct) of the amplification curve. Also perform fecal occult blood testing.

[0132] 2. Standardize the results of manure disposal

[0133] Standardized input reference set data file format, the first column is the sample number, the second to tenth columns are indicator bacteria 1, indicator bacteria 2, indicator bacteria 3, indicator bacteria 4, indicator bacteria 5, indicator bacteria 6, indicator bacteria 7 , ΔCt values ​​of indicator bacteria 8 and indicator bacteria 9, the eleventh to nineteen...

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Abstract

The invention discloses a kit for identifying colorectal cancer. The kit is characterized by comprising a specific primer combination for identifying indicating bacteria, wherein the primer combination is shown as SEQ ID NO.1-12, and the indicating bacteria are a combination of F. nucleatum, P. anaerobius, C. symbiosum, E. faecalis, P. asaccharolytica and S. salivaricus. The invention also discloses an application of the kit. The kit has the beneficial effects that risk prediction of colorectal cancer can be carried out only by collecting stool samples. The principle is to detect colorectal cancer pathogenic bacteria in feces, wherein the risk assessment sensitivity can reach 92% and the specificity is 95%.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to a kit for differentiating colorectal cancer and its application. Background technique [0002] Among the common types of human cancers, according to the statistics published in "Cancer incidence and mortality in China, 2014", the incidence rate of colorectal cancer is 17.2 / 105, ranking fifth in the incidence rate of tumors (the top four are lung cancer, breast cancer, and breast cancer respectively). cancer, stomach cancer, liver cancer). At the same time, colorectal cancer is also one of the most common causes of death. According to statistics, more than 600,000 people die from colorectal cancer every year. [0003] The occurrence of colorectal cancer involves progressive changes in intestinal flora structure, molecular genetics, and related histomorphological changes. It is a multifactorial process that interacts with internal factors and external factors such as the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/14C12Q1/04C12R1/01C12R1/46C12R1/145
CPCC12Q1/689
Inventor 孙子奎宣涛
Owner 南京派森诺基因科技有限公司
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