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A kind of clam symbiotic fungus Aspergillus fumigatus and its preparation method, extract and application

A technology of symbiotic fungi, Aspergillus fumigatus, used in the fields of fermentation engineering, microbiology, and medicine

Active Publication Date: 2021-08-03
JIANGSU MARINE RESOURCES DEV RES INST LIAN YUNGANG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the research on the active substances of marine fungi is relatively in-depth in the development of medicine, and there are few reports on the antagonistic substances of marine fungi to aquatic pathogens and aquatic product spoilage bacteria

Method used

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  • A kind of clam symbiotic fungus Aspergillus fumigatus and its preparation method, extract and application
  • A kind of clam symbiotic fungus Aspergillus fumigatus and its preparation method, extract and application
  • A kind of clam symbiotic fungus Aspergillus fumigatus and its preparation method, extract and application

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Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: Clam symbiotic fungus Aspergillus fumigatus ( Aspergillus fumigatus ) Isolation and identification of HX-1

[0036] Its separation and purification preparation method is as follows: after heating and dissolving 4% (W / V) glucose, 1% (W / V) peptone and 2% (W / V) agar, add aged sea water to constant volume, and sterilize at 121°C for 20 min to obtain the Sabouraud solid medium; cut the clam with sterile scissors, add sterile water to make a suspension, take 0.1 mL evenly spread on the Sabouraud solid medium, and cultivate in a 28°C incubator until the colony grows. After extraction, colonies were picked and streaked for purification.

[0037] The above-mentioned flower clam symbiotic fungus Aspergillus fumigatus ( Aspergillus fumigatus ) The biological form of HX-1 is described as: it grows rapidly on Sabouraud medium in seawater, cultured at 28°C for 7 days, the diameter of the colony can reach 50-65 mm, the center is slightly raised, there are irregular str...

Embodiment 2

[0039] Embodiment 2: Clam symbiotic fungus Aspergillus fumigatus ( Aspergillus fumigatus ) HX-1 fermented extract

[0040] The clam symbiotic fungus Aspergillus fumigatus ( Aspergillus fumigatus ) HX-1 strain was inoculated into seawater liquid medium, cultured at 160 r / min, 28°C for 7 days, and the symbiotic fungus Aspergillus fumigatus ( Aspergillus fumigatus ) liquid culture of HX-1. The clam symbiotic fungus Aspergillus fumigatus ( Aspergillus fumigatus ) HX-1 liquid culture is filtered to separate the mycelia from the fermentation broth, and the fermentation broth is extracted three times with an equal volume of ethyl acetate to obtain a fermentation extract.

[0041] The seawater liquid culture medium is to add glucose 10 g, maltose 20 g, monosodium glutamate 10 g, yeast extract 3 g, KH 2 PO 4 0.5 g, MgSO 4 ·7H 2 O 0.3 g, aged sea water 1 L.

Embodiment 3

[0042] Embodiment 3: Clam symbiotic fungus Aspergillus fumigatus ( Aspergillus fumigatus ) Antibacterial activity of HX-1 fermentation broth extract

[0043] Preparation of indicator bacteria solution: Vibrio anguillarum ( Vibrio anguillarum ), Vibrio harveyi ( Vibrio Harvey ), Vibrio parahaemolyticus ( Vibrio parahaemolyticus ), Vibrio cholerae ( Vibrio cholerae ), Aeromonas hydrophila ( Aeromonas hydrophila ) or Shewanella putrefaciens ( Shewanella putrefaciens ) respectively made spore suspension with beef extract peptone liquid medium (10 7 per mL), the spore suspension was inoculated into a sterilized 500mL triangular flask containing 200mL beef extract peptone medium according to the inoculum amount of 5%, and placed in a constant temperature shaking incubator at a temperature of 37°C and a speed of 160r / min. Shake culture for 1 day to obtain indicator bacteria solution.

[0044] Bacteriostatic activity test: Pour the beef extract peptone solid medium cooled...

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Abstract

The invention discloses a symbiotic fungus Aspergillus fumigatus, a preparation method, extracts and applications thereof. The symbiotic fungus Aspergillus fumigatus ( Aspergillus fumigatus ) The HX-1 strain was deposited in the China Center for Type Culture Collection on June 15, 2018, and the preservation address is Luojia Mountain, Wuchang, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M 2018377. ( Vibrio anguillarum ), Vibrio harveii ( Vibrio harvey ), Vibrio parahaemolyticus ( Vibrio parahaemolyticus ), Vibrio cholerae ( Vibrio cholerae ), Aeromonas hydrophila ( Aeromonas hydrophila ) and Shewanella spoilage ( Shewanella putrefaciens ) has an inhibitory effect, therefore, the realization of the present invention provides candidate drugs for the research and development of new antibacterial drugs.

Description

technical field [0001] The invention relates to the fields of microbiology, fermentation engineering, and medicine, and in particular to a symbiotic fungus Aspergillus fumigatus ( Aspergillus fumigatus ) HX-1 and its preparation method, as well as the fermented extract prepared from the liquid culture fermentation product of the strain, and the application of the extract in the preparation of antibacterial drugs. Background technique [0002] Due to their complex genetic background, various types of metabolites, and high yield, marine fungi have become the main source of marine microbial natural products. Since the 1990s until now, it has been found in the fermentation products of marine fungi from sources such as seaweed, sponges, mangrove ecosystems, marine sediments, marine molluscs, sea squirts, corals, fish, and other marine animals and plants. More than 2,000 new secondary metabolites, compound types include alkaloids, polypeptides, polyketides, terpenoids, steroids a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A61K36/06A61P31/04C12R1/68
CPCA61K36/06A61P31/04C12N1/14C12N1/145C12R2001/68Y02A50/30
Inventor 郭雷陈慧王鑫彤王乐张菲曹曦许福泉陈建华
Owner JIANGSU MARINE RESOURCES DEV RES INST LIAN YUNGANG
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