A
Streptomyces fradiae NTGA-344 strain with the preservation number of CGMCC No.2074 and a method for preparing 20(S)-
ginsenoside Rh1 (6-O-belta-D-Glucopyranoside-20(S)-
Protopanaxatriol by using the strain to ferment and convert
panaxatriol saponins in various notoginsenosides in a
fermentation cylinder are provided. 1(GA), ISP2,
humic acid (HSG culture medium) and asparagines culture medium are selected as the culture media. In the process of
fermentation, the inventory rating proportion of various notoginsenosides is 0.1% to 10 % (w / v), the
fermentation temperature is 18 to 50 DEG C, the pH value of 0.1% to 35% (w / v)
ammonia is dynamically adjusted to be between 2 and 8, the ventilation ratio is 0.1 to 10 (v / v), the stir speed is 10 to 900r / min, the fermentation time is 5h to 200h, and the macroporous absorbent resin is 0.1% to 30% (w / v). The invention provides the
streptomyces yunnanensis with stable property, simple nutritional requirement and good growth and a practical and convenient fermentation cylinder fermentation and separation extraction process for large-scale fermentation and conversion of various notoginsenoside and preparation of 20(S)-
ginsenoside Rh1. The 20(S)-
ginsenoside Rh1 is prepared by the strain and the technology, thereby having low manufacturing cost, less
impurity and high product yield.