Process of preparing 20(S)-ginsenoside Rh1 with streptomycete fermentation of pseudo-ginseng saponin

Abstract

A Streptomyces fradiae NTGA-344 strain with the preservation number of CGMCC No.2074 and a method for preparing 20(S)-ginsenoside Rh1 (6-O-belta-D-Glucopyranoside-20(S)-Protopanaxatriol by using the strain to ferment and convert panaxatriol saponins in various notoginsenosides in a fermentation cylinder are provided. 1(GA), ISP2, humic acid (HSG culture medium) and asparagines culture medium are selected as the culture media. In the process of fermentation, the inventory rating proportion of various notoginsenosides is 0.1% to 10 % (w / v), the fermentation temperature is 18 to 50 DEG C, the pH value of 0.1% to 35% (w / v) ammonia is dynamically adjusted to be between 2 and 8, the ventilation ratio is 0.1 to 10 (v / v), the stir speed is 10 to 900r / min, the fermentation time is 5h to 200h, and the macroporous absorbent resin is 0.1% to 30% (w / v). The invention provides the streptomyces yunnanensis with stable property, simple nutritional requirement and good growth and a practical and convenient fermentation cylinder fermentation and separation extraction process for large-scale fermentation and conversion of various notoginsenoside and preparation of 20(S)-ginsenoside Rh1. The 20(S)-ginsenoside Rh1 is prepared by the strain and the technology, thereby having low manufacturing cost, less impurity and high product yield.

Description

technical field [0001] The invention belongs to the technical field of biology and medicine, and relates to a kind of Streptomyces fermenting and transforming triol group ginsenosides in various notoginseng saponins, and preparing 20(S)-ginsenoside Rh 1 (6-O-β-D-glucopyranosyl-20(S)-protopanaxatriol, hereinafter referred to as Rh 1 ) process. In particular, an artificially domesticated Streptomyces fradiae NTGA-334 strain is provided, and various notoginseng saponins, separation and extraction of Rh 1 craft. Background technique [0002] Human metabolism experiments show that after oral administration of ginsenosides, only slight oxidation occurs under the action of gastric juice and bile, and cannot be degraded. Only under the action of intestinal bacteria, the glycosidic bonds are broken and a series of metabolites . The latest pharmacological research shows that it is not the ginsenoside itself that is absorbed into the blood and exerts its activity, but the metabolit...

AI Technical Summary

Problems solved by technology

[0003] In the preparation of small-component ginsenosides and ginsenoside metabolites, classical chemical methods (chemical synthesis, acid hydrolysis or alkali cleavage) inevitably produce some side reactions, such as epimerization, hydration and hydroxylation (Han BH, et al: PlantaMed, 1982, 44(3), 146-149; Chen Y, et al: Chem Pharm Bull, 1987, 35(4), 1653-1655); prepared by enzymatic conversion of triol group ginsenosides Rh 1 , has the advantages of high conversion rate and easy operation (Ko SR, et al: Planta Med, 2003, 69 (3), 285-286; Ko SR, et al: Chem Pharm Bull, 2003, 51 (4), 404- 408; Ko SR, et al: BiosciBiotechnol Biochem, 2000, 64(12), 2739-2743.), but the price is expensive, there are problems of inconvenient enzyme source and high cost, so the application is limited

Involving Streptomyces fermentative transformation of triol group ginsenosides in various notoginseng saponins, large-scale preparation of Rh 1 research, there is no literature report at home and abroad

Images