96 results about "Mitogen-activated protein kinase" patented technology

Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the cell wall integrity pathway. Yeast with increased expression of SLT2p, a mitogen activated protein kinase of the MAPK module of the cell wall integrity pathway had increased tolerance to isobutanol. These yeast may be used for improved butanol production.

The present invention provides novel compounds that are capable of inhibiting mitogen activated protein kinase-activated protein kinase-2 and analogues thereof and pharmaceutical compositions and kits that include these compounds.

The present invention is used for evaluating the advantages of haliotis discus hannai. A multi-phase HPLC purification system is used to purify anti-inflammatory peptide from abalone (AAIP, abalone anti-inflammatory peptide). In tandem MS analysis, a fragmentation result shows that the amino acid sequence of the AAIP with nitrogen monoxide (NO) inhibitory activity (IC50=55.8[um]M) is Pro-Phe-Asn-Glu-Gly-Thr-Phe-Ala-Ser (1175.2Da). While the anti-inflammatory effect of RAW264.7 macrophages generated by the stimulus of the AAIP to lipopolysaccharides (LPS) is further studied, and the molecular mechanism is elaborated. The result shows that the AAIP peptide inhibits the nitrogen monoxide (NO) generation induced by the LPS through the expression of inducible nitric oxide synthase (iNOS) by a dose-dependent manner, and the gene transcription of pro-inflammatory cytokines is also obviously reduced, wherein the pro-inflammatory cytokines comprise such as interleukin (IL-1 beta), tumor necrosis factors (TNF-beta) and IL-6. In addition, the AAIP obviously inhibits phosphorylation of mitogen-activated protein kinases (MAPK), such as p-p38 and p-JNK. These results indicate that the AAIP inhibits inflammatory response induced by LPS by intercepting the MAPK pathway of the macrophages. Therefore, the AAIP can be applied to therapeutic drugs for inflammations treatment or healthcare food products.

A method is described for inhibiting mitogen activated protein kinase-activated protein kinase-2 in a subject in need of such inhibition, where the method involves administering to the subject a beta-carboline MK-2 inhibiting compound, or a pharmaceutically acceptable salt thereof.

The invention provides a rapid detection method for mitogen activated protein kinase 1 (Mitogen Activated protein Kinase Kinase 1, MEK1 / MAPKK1) gene mutation. The rapid detection method is realized by the following steps: designing a pair of wild primers at the two ends of an MEK1 gene mutation site and designing a pair of mutation primers on the mutation site; respectively designing wild primers and mutation types of a No.2 exon (MEK1-E2) and a No.3 exon (MEK1-E3) of MEK1; respectively amplifying a wild type template and a mutation type template by the primers; utilizing the wild primers to carry out PCR (Polymerase Chain Reaction) amplification on the two templates; respectively amplifying corresponding wild target segment and a mutant type target segment; mixing the two segments according to different ratios and distinguishing by using an HRM method. Compared with the other methods, the rapid detection method has the advantages of high specificity, high sensitivity, convenience and portability; the flux is high and the cost of each time is low.

The present invention relates to compositions and methods useful in treating diseases and disorders related to protein kinases. In particular, the present invention relates to compositions and methods useful for targeting protein kinases related to mitogen activated protein kinase (MAPK) pathways (e.g., p38 MAPK, JNK, ERK, and upstream and downstream protein kinases) and / or casein kinase (CK) pathways (e.g., CK1δ, and upstream and downstream protein kinases), and diseases and disorders related to MAPK pathways (e.g., p38 MAPK, JNK, ERK, and upstream and downstream protein kinases) and / or CK pathways (e.g., CK1δ, and upstream and downstream protein kinases).

The invention discloses a detection kit for accessorily diagnosing type 2 diabetes. The detection kit comprise a primer used for detecting the single nucleotide polymorphisms of a PDPK1 (Phosphoinositide Dependent Protein Kinase) gene, a GCK (Glucokinase) gene, a PIK3R2 (Phosphoinositide 3-Kinase Regulatory Subunit 2) gene, an MAPK9 (Mitogen Activated Protein Kinase) gene, a CBLC (Cobalamin C) gene, an MKNK2 gene, an FLOT2 gene, a TRIP10 gene and a KRAS gene, and the single nucleotide polymorphisms respectively include rs76318740, rs12702070, rs3736328, rs1363513, rs2965143, rs3810412, rs4795473, rs340141 and rs7311692. According to the detection kit, PCR (Polymerase Chain Reaction) amplification is carried out on the DNA (Deoxyribose Nucleic Acid) of a sample by virtue of the primer of the detection kit, a melting curve of a PCR amplification product is analyzed by using a fluorescent quantitative PCR instrument, then the melting curve is compared with a standard melting curve, and the Tm value of the PCR amplification product is compared, so that a corresponding genotyping result is obtained. The detection kit disclosed by the invention is capable of accessorily detecting and predicting the diabetes of testee and has the advantages of simple detection method, high detection efficiency and high pertinence, thereby promoting the early prevention of the type 2 diabetes and providing the reference for medication treatment.

The invention discloses a culture medium for rat embryonic stem cells. The culture medium comprises basal culture media and additives, wherein the additives comprise differentiation inhibitors and Pluripotin; and moreover, the differentiation inhibitors comprise GSK (Glycogen Synthase Kinase) inhibitors, FGFR (Fibroblast Growth Factor Receptor) inhibitors and MAPK (Mitogen-activated Protein Kinases) inhibitors. The invention also discloses a culture medium kit of the rat embryonic stem cells, a method for culturing the rat embryonic stem cells and applications of the culture medium and the kit, wherein the culture medium kit comprises the basal culture media, the differentiation inhibitors and the Pluripotin. With the adoption of the differentiation inhibitors and the Pluripotin, the culture medium for the rat embryonic stem cells can inhibit the differentiation of the rat embryonic stem cells and can further guarantee the stability of karyotype for reproductive potential.