Detection kit for accessorily diagnosing type 2 diabetes and use method thereof
A detection kit, a technology for type 2 diabetes, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as non-disclosure
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[0080] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0081] A detection kit for assisting in the diagnosis of type 2 diabetes, the detection kit comprises mononuclear genes for detecting PDPK1 gene, GCK gene, PIK3R2 gene, MAPK9 gene, CBLC gene, MKNK2 gene, FLOT2 gene, TRIP10 gene and KRAS gene The primers of nucleotide polymorphisms, the single nucleotide polymorphisms are respectively rs76318740, rs12702070, rs3736328, rs1363513, rs2965143, rs3810412, rs4795473, rs340141 and rs7311692, and the primers include:
[0082] PDPK1 gene-specific upstream primer 1:
[0083] 5'-gcgggcagggcggcCAGCTGCCCCTCTCTCCCc-3';
[0084] PDPK1 gene-specific upstream primer two: 5'-gattaccg CAGCTGCCCCTCTCTCCCt-3';
[0085] PDPK1 gene downstream primer: 5'-AGAAAGCATCTGAGCAGGCACCAG-3';
[0086] GCK gene-specific upstream primer 1:
[0087] 5'-gcgggcagggcggcCCTTGTCACTACAGTGACCGCc-3';
[0088] GCK gene-specific upstr...
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