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Anti-inflammatory peptide separated from haliotis discus hannai abalone visceral organ and use of anti-inflammatory peptide

A technology for abalone viscera and wrinkled abalone, which is applied to medical preparations containing active ingredients, anti-inflammatory agents, peptides, etc., can solve problems such as rarely reported beneficial effects of abalone

Active Publication Date: 2016-03-30
千忠吉
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  • Claims
  • Application Information

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Problems solved by technology

However, although many studies on the biological and physiological properties of abalone have been conducted on pathology, genetics and aquaculture techniques, the beneficial effects of abalone on health are rarely reported (Sun et al., 2010; Ekanayake et al., 2008 ; Zoysa et al., 2009; Wan, Whang, & Lee, 2005)

Method used

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  • Anti-inflammatory peptide separated from haliotis discus hannai abalone visceral organ and use of anti-inflammatory peptide
  • Anti-inflammatory peptide separated from haliotis discus hannai abalone visceral organ and use of anti-inflammatory peptide
  • Anti-inflammatory peptide separated from haliotis discus hannai abalone visceral organ and use of anti-inflammatory peptide

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[0015] In order to make the purpose, technical solution and advantages of the present invention clearer, the specific implementation manners of the present invention will be described in detail below. Apparently, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0016] materials and sources

[0017] Macrophages (RAW264.7) were obtained from American Type of Culture Collection (Manassas, VA, USA). Dulbecco's modified Eagle's medium (DMEM), trypsin-EDTA, penicillin / streptomycin / amphotericin (10,000 U / mL, 10,000 μg / mL, and 2,500 μg / mL, respectively), phosphate buffer Saline (PBS) and fetal bovine serum (FBS) were obtained from GibcoBRL, Life Technologies (USA). 3-(4,5-Dimethyl-2-yl)-2,5-diphenyltetrazolium bromid...

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Abstract

The present invention is used for evaluating the advantages of haliotis discus hannai. A multi-phase HPLC purification system is used to purify anti-inflammatory peptide from abalone (AAIP, abalone anti-inflammatory peptide). In tandem MS analysis, a fragmentation result shows that the amino acid sequence of the AAIP with nitrogen monoxide (NO) inhibitory activity (IC50=55.8[um]M) is Pro-Phe-Asn-Glu-Gly-Thr-Phe-Ala-Ser (1175.2Da). While the anti-inflammatory effect of RAW264.7 macrophages generated by the stimulus of the AAIP to lipopolysaccharides (LPS) is further studied, and the molecular mechanism is elaborated. The result shows that the AAIP peptide inhibits the nitrogen monoxide (NO) generation induced by the LPS through the expression of inducible nitric oxide synthase (iNOS) by a dose-dependent manner, and the gene transcription of pro-inflammatory cytokines is also obviously reduced, wherein the pro-inflammatory cytokines comprise such as interleukin (IL-1 beta), tumor necrosis factors (TNF-beta) and IL-6. In addition, the AAIP obviously inhibits phosphorylation of mitogen-activated protein kinases (MAPK), such as p-p38 and p-JNK. These results indicate that the AAIP inhibits inflammatory response induced by LPS by intercepting the MAPK pathway of the macrophages. Therefore, the AAIP can be applied to therapeutic drugs for inflammations treatment or healthcare food products.

Description

technical field [0001] The invention belongs to the field of biotechnology, specifically the field of bioactive peptides. In particular, the present invention relates to anti-inflammatory peptides isolated from abalone intestines using a continuous HPLC purification system and uses thereof. Background technique [0002] Inflammation represents a highly coordinated set of events that allows a tissue to respond to injury or infection, requiring the participation of various cell types to express and respond to a variety of mediators in a sequential fashion (Sebban & Courtois, 2006). Macrophages are the main immune cells in the innate immune system. Activation of macrophages plays a key role in the inflammatory response when infected by pathogens. Macrophages can kill pathogens directly by phagocytosis and indirectly by secreting various pro-inflammatory mediators. Macrophages play an important role in inflammatory diseases involving the overproduction of proinflammatory cyto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/20C07K1/18C12P21/06A61K38/08A61P29/00A23L33/18
Inventor 千忠吉苏伟明
Owner 千忠吉
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