Cloning of poncirustrifoliata mitogen-activated protein kinase (PtrMAPK) and application of PtrMAPK to improvement of drought resistance of plant

Abstract

The invention belongs to the field of plant genetic engineering, and particularly relates to a poncirustrifoliata mitogen-activated protein kinase (PtrMAPK) gene which is obtained from poncirustrifoliata by separation and cloning. The nucleotide sequence of the gene is shown as a sequence table SEQ ID NO:1; the corresponding amino acid sequence of the gene is shown as a sequence table SEQ ID NO:2; and the gene comprises 1128bp of open reading frame, and encodes 375 amino acids, an isoelectric point is 5.51, and predicted molecular weight is 43kD. The PtrMAPK gene is imported into tobacco for functional verification, and the drought resistance capacity of the obtained transgenic tobacco plant can be improved obviously. New gene resources are provided for anti-abiotic stress molecular design and breeding, new genetic resources are provided for implementing green and environment-friendly agriculture, and the development and utilization of the genetic resources contribute to reducing agriculture production cost and achieving environment-friendliness.

Description

technical field [0001] This patent belongs to the field of plant genetic engineering. Specifically, it involves isolating and cloning the mitogenic protein kinase gene PtrMAPK from Poncirus trifoliata, and then introducing the gene into the model plant Nicotiana tabacum for functional verification, and the obtained transgenic plants have significantly improved drought resistance. Background technique [0002] During the growth and development of plants, plants are often affected by adverse external environments, among which drought has been proven to be the most destructive abiotic stress, which seriously affects the growth, development and yield of plants. However, when plants are stimulated by an unfavorable external environment, they do not accept it passively. In fact, during the long process of evolution, plants have established a set of mechanisms to respond to adversity stress, which involves the plant's perception of adversity signals, the transmission of adversity ...

AI Technical Summary

Problems solved by technology

[0005] It must be pointed out that although MAPKs have been cloned from different plants, the functions of most protein kinases under drought stress still need to be further verified

On the other hand, it is worth noting that most of the information about MAPK comes from Arabidopsis, rice, tobacco, and tomato, but there is very little information about MAPK in fruit trees.

Studies have shown that although the MAPK signaling pathway has a certain degree of conservation in the evolution of eukaryotes, the composition of this cascade and the functions of specific components are somewhat different (Morris, 2001; et al., 2010). Hovenia trifoliate is a widely used rootstock in the citrus industry, but it is susceptible to drought, which leads to its use in areas where water resources are limited and droughts occur periodically

Although many studies have shown that the MAPK gene has been cloned in some plants, there is no report on the cloning and functional verification of the gene in Citrus trifoliate, let alone the literature report on the application of the MAPK gene in Citrus trifoliate to transform plants to improve drought resistance

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