The invention discloses a novel
metal cup-cylinder composite nano structure array which is formed by sequential arrangement of
metal nano structure array units. The
metal nano structure array units are of a metal cup-cylinder composite nano structure which comprises a cup-shaped metal nano outer wall, and the center of a cup groove formed by the metal nano outer wall is a cylindrical metal nano particle. The invention also discloses a preparation method of the metal nano structure array, and the preparation method comprises the
processing steps of preparing a single-layer orderly polrvinyl
benzene nano ball compact arrangement, preparing a single-layer orderly polrvinyl
benzene nano ball non-compact arrangement, preparing a nano annular-structure array
mask made of composite materials, preparing a
silicon-nano-structure array template, preparing the metal cup-cylinder composite nano-structure array, and the like. The metal nano array structure provided by the invention has gaps with smaller nano sizes and can be used for realizing the Roman detection of a single
biomolecule; and according to the preparation method, the compatibility is good, the efficiency is high and the cost is low.