52 results about "Genetic Technique" patented technology

The present invention concerns methods and kits for diagnosing a disease condition characterized by non-physiological levels of hepcidin, comprising obtaining a tissue or fluid sample from a subject; contacting the sample with an antibody or fragment thereof that specifically binds to a polypeptide corresponding to the mid-portion or C terminus of a hepcidin protein, and quantifying the hepcidin level using an assay based on binding of the antibody and the polypeptide; wherein the non-physiological level of hepcidin is indicative of the disease condition. The present invention also concerns diagnostic methods and kits for applications in genetic technological approaches, such as for overexpressing or downregulating hepcidin. The present invention further concerns therapeutic treatment of certain diseases by treatment of subjects with hepcidin and agonists or antagonists of hepcidin.

The invention provides an avian influenza virus D3 / F-R2 / 6*reassortment and construction method thereof, which is related to the field of reversal genetic technology and comprises, reassorting the six internal genes of the MPAIV chicken embryonic highly adaptive strain A / Chicken / Shanghai / F / 98(H9N2) PB2, PB1, PA, NP, M, NS and the NA gene and attenuate HA geine of the HPAIV epidemigenic strain A / Duck / Huadong / D3 / 00 (H5N1), thus constructing an avian influenza virus D3 / F-R2 / 6*reassortment, which can be used for manufacturing vaccine for treating avian influenza.

Embodiments of the present invention provides a system that optimizes a regression model which predicts a signal as a function of a set of available signals. These embodiments use a genetic technique to optimize the regression model, which involves using a portion of the sample signals used to generate each parent regression model from a pair of best-fit parent regression models to generate a child regression model. In addition, in embodiments of the present invention, the system introduces “mutations” to the set of sample signals used to create the child regression model in an attempt to create more robust child regression models during the optimization process.

The invention discloses a method for utilizing Nsp2 gene deletion for constructing a PRRSV gene deletion vaccine virus strain and the application thereof. The method for lowering the toxicity of the PRRSV BJ-4 strain of the invention is that a nucleotide segment with the length of 69nt in a Nsp2 coding region of the PRRSV BJ-4 strain genome is continuously deleted to obtain the virus strain with reduced toxicity; the nucleotide sequence of the 69nt nucleotide segment is shown as the 2795 to 2863 sites from the 5' tail end of GenBank Accession Number AF331831. The Nsp2 of the virus rV63 which is prepared by the method for lowering the toxicity of the PRRSV BJ-4 strain deletes 21 amino acids, which has the potential to develop a marker vaccine and can identify the diagnosis method of the deleted polypeptide. When in the development process of vaccine, the invention can utilizes a reverse genetic technical platform which is already established for carrying out the transformation of GP5 glycosylation sites, thus overcoming the suppression factors which affect the neutralizing epitope and further improving the level of the vaccine-induced neutralizing antibody.

The invention provides a method for isolating or enriching a rare cell from a biological fluid of a mammal employing an antibody that binds a cell-surface antigen of the rare cell. The immobilized antibody is incubated with a sample of biological fluid that includes the rare cells and a plurality of other cells so as to form an antibody-rare cell complex. The complex can be detected or isolated and subsequently analyzed by any of a variety of physical, chemical and genetic techniques.

The invention provides an entire-gene cDNA clone of modified measles virus, which is obtained from the substitution and transformation of main surface antigen genes (H gene) of main existing epidemic strains (genotype of H1 and representative strain of China93-7) in China to the full-length cDNA clones of measles virus strain CC-47. The invention adopts the RNA virus reverse genetic technique to carry out the rescue of modified CC-47 virus on the cDNA level so as to obtain modified infective virus, thus providing great convenience for the research on the replication, the proliferation and the package of novel virus stains. In addition, the novel produced infective virus has the hemagglutinin antigen of an epidemic strain and has more suitable application value of the hoplivac.

The native chicken marking and mating technique is belongs to the field of domestic bird genetic technique. The Beijing oil chichen has golden genetic genes, phoenix head, hair feet and five toes. Take it as the paternal to cross with the high layer hen which has silver genes, and the main strain is the import high-yield mating female line hen(such as roman egg chicken, halen egg chicken and yisa egg chicken). The hens of the filial generation are having golden feathers, while the cocks are having efflorescence feathers, so the day-old chick's sex can be distinguished by the feather. The eggs produced by the hens of filial generation, basically contains the character of the crossing imitation eggs. It improves the egg-laying property substantially, reduces the cost of production, and has great economic benefit and social benefit.

The present invention concerns methods and kits for diagnosing a disease condition characterized by non-physiological levels of hepcidin protein, including prohepcidin and fragments thereof, comprising obtaining a tissue or fluid sample from a subject; contacting the sample with an antibody or fragment thereof that specifically binds to a polypeptide corresponding to the mid-portion or C terminus of a hepcidin protein, and quantifying the hepcidin level using an assay based on binding of the antibody and the polypeptide; wherein the non-physiological level of hepcidin is indicative of the disease condition. The present invention also concerns diagnostic methods and kits for applications in genetic technological approaches, such as for overexpressing or down-regulating hepcidin. The present invention further concerns therapeutic treatment of certain diseases by treatment of subjects with hepcidin and agonists or antagonists of hepcidin.

The present invention relates generally to recombinant genetic technology. More particularly, the present invention relates to compositions and methods for use in selection and isolation of nucleic acid molecules. The invention further relates to methods for the preparation of individual nucleic acid molecules and populations of nucleic acid molecules, as well as nucleic acid molecules produced by these methods. The invention also relates to screening and / or selection methods for identifying and / or isolating nucleic acid molecules which have one or more common features (e.g., characteristics, activities, etc) and populations of nucleic acid molecules which share one or more features.

The present invention relates to genetic techniques employing the direct ligatation of an external DNA fragment generated in situ by the same ZFNs that target the genome. ObLiGaRe, i.e., the obligated ligation-gated recombination, is a new method for genetic engineering using custom designed nucleases, and a strategy of site-specific gene insertion utilizing the NHEJ pathway. It applies a similar logic to the one used in unidirectional loxP sites (Oberdoerffer et al., 2003) but maintains all the advantages and flexibility of CDNs.

The invention provides a method for isolating or enriching a rare cell from a biological fluid of a mammal employing an antibody that binds a cell-surface antigen of the rare cell. The immobilized antibody is incubated with a sample of biological fluid that includes the rare cells and a plurality of other cells so as to form an antibody-rare cell complex. The complex can be detected or isolated and subsequently analyzed by any of a variety of physical, chemical and genetic techniques.

The present invention relates to a mutated virus. Said virus can be an influenza virus of human or other animal origin. The present invention also relates to a method for preparing the mutated virus, the method comprising introducing UAG codons into positions upstream of the stop codons per se of one or more genes of a viral genome by reverse genetic techniques. The present invention further relates to uses of the mutated virus, for example, as a live attenuated vaccine, or in replication of controllable and safe virus models, and the like.

The invention discloses an oncolytic virus NDV-NRP1 and a construction method and application thereof, and belongs to the technical field of microorganisms. A recombinant virus NDV-NRP1-scFv-GT is constructed by adopting a reverse genetic technology, specific replication of rNDV in tumor cells is achieved at the transcriptional level by using an hTERT promoter, by combining NRP1-scFv with NRP1, invasion and growth of tumors are inhibited, and alpha-Gal epitope is overexpressed, so that anti-Gal of a human body can be induced to specifically target the tumor cells, complement is activated, killing of the tumor cells is achieved, and an effective anti-tumor effect is achieved.

The invention discloses a method for quickly constructing an IBV (Avian Infectious Bronchitis Virus) reverse genetic strain, and belongs to the technical field of coronavirus reverse genetics. The constructing method comprises the following steps: quickly completing construction containing IBV genomic full-strength cDNA (Complementary Deoxyribose Nucleic Acid) clone by taking a BAC (Bacterial Artificial Chromosome) vector as a framework and applying an in-vitro homologous recombination technology, directly transfecting cells by a constructed recombinant plasmid, and transcribing in the cells, thus obtaining a transcript having infectivity; completing virus packaging; inoculating SPF (Specific Pathogen Free) chick embryo to a mixed solution of the cells and a culture medium and passing from generation to generation, thus obtaining the IBV reverse genetic strain. The constructing method disclosed by the invention has the advantages of simple operation and high positive cloning efficiency, the obtained IBV reverse genetic strain has passage stability, and an effective tool is provided for researching pathogenesis of the virus in vitro, developing a novel vaccine and the like; according to the method disclosed by the invention, transcription is carried out in the cells by utilizing a CMV (Cytomegalovirus) promoter added on a 5' terminal, and the rescue efficiency of the virus is greatly increased by utilizing an HDVR (Hepatitis Delta Virus Ribozyme) sequence added on a 3' terminal.

The invention discloses a research method based on the genetic homozygosity of SD rats, including preparatory work, first-generation breeding, first-generation mating, first-generation cultivation, second-generation birth, second-generation mating, and third-generation birth. The four groups of SD rats are wild female SD rats. Rats and wild male SD rats, artificially raised female SD rats and artificially raised male SD rats, wild female SD rats and artificially raised male SD rats, wild male SD rats and artificially raised female SD rats. The present invention relates to the field of SD rat genetic technology. Based on the SD rat genetic homozygosity research method, wild female SD rats and artificially raised male SD rats, wild male SD rats and artificially raised female SD rats can The wild SD rats and artificially raised SD rats were compared and mated. Among the offspring produced in this way, the staff can more intuitively observe the genetic homozygosity of different types of SD rats and increase the accuracy of the experiment.