58 results about "Folch solution" patented technology

A method, apparatus and kit for precisely and accurately determining the volume of a liquid aliquot which can be used to calibrate a liquid delivery device. Two dye-containing solutions, a sample solution and a diluent solution, are prepared such that each solution contains a different dye, with the solutions having different absorbance values at a first wavelength and at a second wavelength. The absorbance value of a known volume of diluent solution is measured at both wavelengths. Either one aliquot of sample solution is added to the diluent solution and then absorbance measurements are made at both wavelengths, or multiple aliquots of sample solution are mixed serially into the diluent solution and then absorbance measurements are made at both wavelengths after each aliquot is added. The volume of any single aliquot is calculated by using a two-step formula which is based on the Beer-Lambert law.

One embodiment of the present invention is a diffusion continuous batch cell-free protein-synthesis method characterized simultaneously by continuously supplying substrate and energy source molecules in the supply phase to the reaction phase by the free diffusion via interface between both phases and by transferring by-products formed in the reaction phase by enhancing the efficiency of the synthesis reaction by prolonging the reaction lifetime by directly contacting a synthesis reaction mixture (reaction phase) containing a biological extract with a substrate- and energy source-supplying solution (supply phase) without using barrier such as semi-permeable membrane or ultrafiltration membrane in a general cell-free protein-synthesis reaction means. Another embodiment of the present invention is a dilution batch cell-free protein synthesis method characterized by enhancing the efficiency of the protein synthesis by prolonging the reaction lifetime by adding a diluting solution to the reaction mixture after pre-incubating the reaction mixture in a cell-free protein-synthesis reaction means using a wheat-embryo extract. Another embodiment of the present invention is a method characterized by enhancing the efficiency of the synthesis reaction simultaneously by re-supplying substrate and energy sources necessary for the protein synthesis (e.g., amino acids, ATP, GTP, creatine phosphate) to the reaction mixture using a gel filtration column and / or semipermeable membrane and by discontinuously removing by-products formed during the reaction after the synthesis reaction stops in the batch cell-free protein synthesis method.

The invention relates to a hydrogenation catalyst carrier the concentrations of acid additives of which are distributed in gradient reducing, and a preparation method thereof; the preparation method comprises the following steps: adopting Al2O3 or Al2O3 containing SiO2, TiO2 and ZrO2 as a carrier, preparing a solution of higher concentration of the acid additives, and saturating and spraying the carrier by gradually adding deionized water to dilute the solution in the process of soaking the carrier; or preparing at least two kinds of solutions of different concentrations of the acid additives, and then spraying the solutions on the carrier in a descending order of the concentration of the acid additives; then drying for 1-8 hours at 80-150DEG C, and then roasting for 2-6 hours in air at 300-650DEG C; the acid additives are compounds containing P, B or F; and the preparation is simple, the manufacturing cost is low, the carrier is used as a carrier for a heavy-oil hydrogenation catalyst, the preparation method is simple, the manufacturing cost is low, the acidity and pore properties of the carrier can be effectively modified, and the acidity of the catalyst can be effectively modified and / or the interaction between active components and the carrier is improved.

The invention relates to a shelf stable liquid disinfectant concentrate composition containing at least 1% by weight of a quat biocide (biocidally active quaternary ammonium compounds) and capable of dilution with 20 parts of water to 1 part of concentrate to produce a diluted solution, the diluted solution exhibiting a MIC after 24 hours in the presence of up to 2% tryptone (or the protein equivalent thereof) which is less than the MIC of a simple solution of the same concentration of the same quat biocide in water in the presence of the same concentration of the protein. The biocidal efficacy of the quat biocide may be protected by an "activity protector" selected from the group consisting of "enzyme stabilisers", "enzyme stabilising systems", micelle formation modifiers and inhibitors, and combinations thereof. The invention also relates to a disinfectant working solution prepared from the concentrate, and to a method of protecting a quat biocide from deactivation.

The invention relates to an enzyme immobilization method based on a hyperbranched polymer, which belongs to the technical field of bioengineering. The method comprises: in a synthetic hyperbranched polymer solution with a large number of diazonium salt groups on the periphery, or In the dilute solution of the hyperbranched polymer solution diluted with water or organic solvent N,N-dimethylformamide less than 100 times, the carrier is soaked for more than half an hour; then the carrier is taken out, washed with water, and then immersed in In the enzyme-containing buffer solution, adsorb at 4°C for more than 4 hours to achieve saturated adsorption; this method can greatly simplify the operation steps of immobilized enzymes, without the need for pre-modification of materials and enzymes, and has high universality; it can be used On a variety of enzymes and carriers, and has no obvious effect on the catalytic activity of the enzyme.

The invention provides a synthetic method of a cell-free protein, which connects synthesis reaction solution (reaction phase) containing organism extract to substrate and energy source supplying solution (supplying phase) directly, and free diffuse substrate and energy source atoms of supplying phase by connecting surface, continuously supplying to the reaction phase; and discharge the by-products of reaction phase to the supplying phase to prolong the duration of the reaction. The invention prolongs the duration of the reaction by adding dilution solution to the solution containing wheat germ extracts. The invention re-supply amino acids, ATP, GTP and fiber phosphoric acid for protein synthesis by gel filtration column and translucent film in the solution after synthesis reaction, and discontinuously discharge the by-products of the reaction.

An aqueous solution is prepared comprising 5 to 25% by weight of an anionic surfactant, 0.5 to 20% by weight of an amphoteric surfactant, 4 to 6% by weight of a nonionic surfactant and, if necessary, an antiseptic and the aqueous solution is adjusted with an organic acid to pH in the range of 4.5 to 7.0, which is used as an undiluted solution of a hydrophilicity maintaining and promoting agent for a polysilazane-containing coating film. The undiluted solution is diluted with water to from 3 to 15 times and the resulting solution is used as a hydrophilicity promoting agent. While the undiluted solution is diluted with water to from 30 to 70 times and the resulting solution is used as a hydrophilicity maintaining agent. The hydrophilicity promoting agent is applied on an anti-fouling coating film which is formed by applying on a substrate a coating solution comprising polysilazane and, if necessary, silica conversion catalyst, and thereby the coating film is made hydrophilic in an extremely short time. Further the hydrophilicity maintaining agent is used for removing stains which adhere to the coating film and maintaining the hydrophilicity of the film.

The invention discloses a determination method of hesperidin content, which comprises the following steps of: (1) preparing a dilute solution; (2) preparing an extract solution; (3) preparing a reference solution; (4) preparing a test sample solution; (5) determining chromatographic conditions; and (6) detecting a program. The determination method of the hesperidin content disclosed by the invention is convenient, simple and fast to operate, good in specificity and repeatability, high in recovery ratio, can accurately determine the hesperidin content, and is convenient for controlling qualities of citrus bioflavonoids and vitamin C complex preparations.

Methods and systems for diluting a dose of a substance to be delivered to a patient and for determining a maximum deliverable dose of the substance and / or a partial fluid volume to be delivered to the patient to provide a desired dose amount are provided. The method includes the step of providing a first substance within a fluid reservoir having an initial unit value. The method also includes diluting the first substance with a second substance to produce the diluted solution. Once the first substance is diluted, a total volume (VTotal) of the diluted solution is measured. A concentration of the diluted solution is then calculated based on the initial unit value and the total volume (VTotal). A fluid delivery system including a fluid reservoir and fluid delivery device, which is configured to perform the method, is also provided.

A fecal analyzing apparatus includes an extraction / dilution section (43) for extracting a fecal suspension from a feces sampling container (1). The fecal suspension is formed by dispersing feces in a feces-dissolving liquid in the feces sampling container (1), and mixing a diluting solution having a different concentration than the feces-dissolving liquid, with respect to a specific pigment, into the extracted fecal suspension in a predetermined amount. An optical-absorbance measurement section (61) detects the concentration of the specific pigment to be changed in response to the addition of the diluting solution, and a control device (66) determines the adequacy of the extraction amount of the fecal suspension in accordance with the detected concentration of the specific pigment. The adequacy of the extraction amount of the fecal suspension can be determined to provide an adequate assay result.

The invention features dilute solutions of rigid rod or extended rod lyotropic liquid crystalline polymers prepared from high concentration polymerization mixtures. The invention also features methods of preparing such dilute solutions which utilize high mechanical shear to induce mix the high concentration solution with a diluent. The invention further provides articles of manufacture, including films and fibers, having a porous microstructure which are prepared from the dilute homogeneous solutions of the invention.

Activated aluminum sesquichlorohydrate (AASCH) powders and method of making are disclosed. The AASCH powder has Al:Cl atomic ratio of from about 1.60 to about 1.90 and Band III polymer concentration of at least about 20% and Band IV polymer concentration of at least about 15% when analyzed with Size Exclusion Chromatogram (SEC) operated by High Performance Liquid Chromatograph (HPLC). The method of making the active comprises (a) diluting the concentrated aluminum sesquichlorohydrate (ASCH) solution to from about 10% to about 25% by weight and (b) heating the diluted solution to obtain a Band III polymer concentration of at least about 20% and a Band IV polymer concentration of at least about 15%, and (c) drying the heated solution to powders having Al:Cl ratio of about 1.60 to about 1.90 and (d) optionally screen or light mill the powders to free flowing spherical particles.

The invention provides an isonaringin extraction and separation process, which belongs to the technical field of plant extraction. The isonaringin extraction and separation process comprises the following steps: (1) carrying out reflux extraction on an extraction raw material by using an extraction agent to obtain a flavone concentrated solution; (2) adjusting the pH value of the flavone concentrated solution to 7.5-8.5 by using an inorganic acid, standing, carrying out suction filtration, and taking a mother solution, namely, a liquid containing isonaringin; (3) continuing to adjust the pH value to 5-7 with an inorganic acid, uniformly stirring, carrying out crystallizing, and carrying out suction filtration to obtain a crude isonaringin product; (4) adding the crude isonaringin product into a polar organic solvent, heating the organic solvent to dissolve the crude isonaringin product, carrying out filtering to remove insoluble impurities, carrying out concentrating and crystallizing,carrying out suction filtration, carrying out washing with water, and carrying out drying to obtain a semi-finished isonaringin product; and (5) dissolving the semi-finished isonaringin product witha polar organic solvent, stirring for dissolving, carrying out filtering to remove impurities, adding pure water to dilute the solution, stirring, standing for crystallization, carrying out suction filtration, carrying out washing with pure water to neutrality, and carrying out drying to obtain a yellow isonaringin product. Isonaringin is obtained through extraction, separation and refining, the content reaches up to 90% or above, and the purity is 95% or above.

The invention provides a method of preparing a biological components-containing solution suitable for clinical proteome analysis by mass spectrometry, electrophoresis, liquid chromatography or the like and an apparatus for the method. The method of the invention is a method for combining two steps among steps of (1) adsorbing proteins having a molecular weight equal to or higher than that of albumin; (2) fractionating proteins having a molecular weight equal to or higher than that of albumin; and (3) concentrating proteins: or a method for preparing the solution by separation using a membrane separation unit having a comparative permeation ratio of 50 or higher for proteins with a molecular weight less than 15,000 and proteins with a molecular weight of 60,000 or higher; or a method for preparing the solution by introducing a biological components-containing raw solution into a separation membrane module and successively passing a diluting solution for circulating the solution and passing a portion of the solution through a separation membrane.