46 results about "Protein species" patented technology

The present application discloses acolloidal nanoparticle that includes a therapeutic nucleic acid species and a targeting protein species attached via a coating on the nanoparticle that facilitates the specific attachment of both species.

The invention provides a preparation method of a yeast cell extracting solution, the yeast cell extracting solution and a method for synthesizing protein in an in vitro system by virtue of the yeast cell extracting solution, as well as a kit containing the yeast cell extracting solution. The kit, which is more convenient than a conventional method, is applicable to protein synthesis in the in vitro system; transformation, culture and crushing can be prevented; a great amount of using time and cost can be saved; various types of proteins can be expressed, and influence of protein toxicity can be prevented; and a plurality of protein complexes can be expressed, without the use of a high temperature at 37 DEG C. In addition, as a raw material adopted by the invention, yeast cell is simple to cultivate, convenient to operate, rapid to propagate and relatively low in cost; a prepared yeast extract has a capacity of modifying translated protein, the yeast extract is suitable for large-scale preparation and has an advantage of industrial production; and the yeast cell extract, which is prepared into freeze-dried powder by virtue of a vacuum freeze-drying method and is preserved at low temperature or high temperature, is convenient to transport.

The invention relates to protein-containing composition, in particular to protein-containing composition and food for pregnant women as well as a preparation method and an application of the composition. The composition is prepared from soy protein isolate, whey protein, pea protein, yeast protein and docosahexaenoic acid powder. The invention provides high-quality protein powder suitable for pregnant women, and protein is widely sourced and includes soy protein and pea protein which are vegetable protein, whey protein which is animal protein as well as yeast protein which is microbial protein. Compared with existing food for supplementing pregnant women with protein nutrition, the product has the advantages that matching of protein types is matched more reasonably, and more nutrients contributing to health keeping of pregnant women and fetuses exist in the composition.

Abstract of the Disclosure The present invention provides a variety of related proteomics analytical modalities that are open-ended, rapid, convenient and suitable for implementation in a high throughput parallel assay system. Specificity-determining compositions and methods are disclosed for use in proteomics. These compositions and methods provide a protein resolved from other protein species contained in a sample fluid, in its native, biologically functional conformation. The present invention provides a specificity-determining substrate that forms a complex with a protein molecule in a homogenous fashion. The specificity-determining substrate includes a specificity-determining ligand bound to a support, wherein optionally the substrate further includes a spacer bound between the ligand and the support. In addition a complex is provided that includes a specificity-determining substrate and a protein molecule. Furthermore, an array including a plurality of loci is provided, in which each locus includes a specificity-determining substrate of the invention. These substrates, complexes and arrays may be employed in a method of resolving a first protein from a fluid including one or more species of native, biologically active protein molecules, wherein the first protein retains its native structure and its biological activity; in a method of purifying one or more first proteins from a fluid including one or more species of native, biologically active protein molecules, wherein the purified first protein retains its native structure and its biological activity; in a method of characterizing one or more proteins in a fluid including one or more species of protein molecule; and in a method of identifying one or more proteins in a sample fluid wherein the concentration of the one or more proteins in the sample fluid differs from the concentration of the one or more proteins in a reference fluid.

Methods are provided for intein mediated trans-splicing of immobilized polypeptides and for producing cyclic polypeptides in vivo or in vitro.

The invention provides a fig soy sauce. The fig soy sauce has nutrient components of figs, is bright in color and glossy, has rich sauce fragrance and ester fragrance and faint fig fragrance, is fresh, delicious and mellow in flavor, fresh, sweet and palatable, unique in style and rich in nutrition, and provides a new variety for soy sauce families. Compared with a conventional high-salt liquid soy sauce, the fig soy sauce has the advantages that the figs are added, so that the kinds of protein are increased, more carbohydrate and more vitamins are increased, and growth and metabolism of various microorganisms are facilitated; besides, cancer resisting components in the figs are added, and fig slurry is subjected to enzymolysis of aspergillus FJ036, so that components of protein, fibers and the like in the figs can be fully utilized, and the utilization effect of fig components can be effectively improved.

Methods for enhancing separation and analysis of biological molecules, particularly proteins, and for characterizing tissue, cell, and subcellular (e.g., organelle) expressed protein profiles (proteomes or protein fingerprints) are disclosed. Multi-dimensional diagrams that illustrate the characteristics of the proteins in a sample, based at least in part on interactions between proteins in the system can be produced. In certain embodiments, the diagrams are three-dimensional and incorporate information on protein-protein interactions, protein charge, and protein size for substantially all of the protein species in the sample. Also described are methods of using the provided multi-dimensional diagrams to detect changes in biological systems that are for instance due to disease, drug treatment, environmental condition, and so forth. Methods are provided for correlating changes in three-dimensional proteomic diagrams to disease diagnosis and prognosis, toxicology, therapeutic compound (e.g., drug or hormone) efficacy and mode of action, and drug design.

The invention provides a method for preparing a fodder by utilizing shrimp leftovers. The method comprises the following steps: 1) preprocessing; 2) activating; 3) primarily fermenting; 4) preparing compound albumen powder; and 5) preparing the fodder. The method has the beneficial effects that the strong acid production capacity and enzyme production capacity of lactobacillus bulgaricus and bacillus subtilis are utilized to treat the shrimp leftovers under the assistance of additive 1; protein and calcium carbonate are removed from the shrimp shells; according to the method, the protein extraction rate is high and the decalcification rate of the shrimp shells is high; the bacillus subtilis is compounded into alpha-amylase and protease which can degrade the vegetable protein and decompose the vegetable protein inhibitors; the vegetable protein in bean pulp is decomposed into quickly absorbed and digested small molecular weight protein and amino acid, the protein category is enriched and the fodder nutrition is more complete; and the preparation method is simple in operation, high in protection extraction rate, high in activity of extracted protein and high in economic benefit.

The invention discloses a method for extracting xyloglucan from tamarind seeds, and belongs to the technical field of plant polysaccharide extraction. According to the diversity of protein types in tamarind seeds and the characteristics of acid resistance, salt resistance and heat resistance of tamarind xyloglucan, the tamarind seeds are pretreated to obtain tamarind polysaccharide coarse powder, and then protein in polysaccharide is removed through acid treatment and alkali treatment in sequence in combination with activated carbon adsorption. And dialyzing under a sterile condition to finally prepare the ultra-pure tamarind xyloglucan with low protein content and low bacterial endotoxin content. Compared with an existing water extraction and alcohol precipitation method and an organic acid extraction method for extracting tamarind xyloglucan, the polysaccharide prepared through the method has the advantages of being good in solubility, high in purity and the like, application of the tamarind xyloglucan in the field of biological medicine is greatly promoted, alcohol is not needed to serve as a settling agent, procedures are reduced, and cost is reduced.

The invention relates to a method for screening biomarkers of ionizing radiation as well as an application of SZT2 protein determined by the method to preparation of a molecular biomarker kit for personnel exposed to heavy ion radiation. The method comprises the following steps: screening differentially expressed genes in human cells which are treated with heavy ion irradiation by using a genechip method; screening differentially expressed proteins in cells of the same type which are treated with heavy ion irradiation by using iTRAQ technology; comparing screening results, and from the genes and the proteins which are differentially expressed simultaneously, selecting differentially expressed protein SZT2 which is down-regulated both in gene and protein levels as the biomarker of ionizing radiation. The genechip method and iTRAQ technology are combined, categories and quantities of genes and proteins in cell specimens of experimental groups of different dosages and a control group are compared and analyzed, the differentially expressed protein SZT2 which is down-regulated both in gene and protein levels can be rapidly found, and the protein can be used as a significant biomarker of ionizing radiation.

The invention relates to a processing method of highly-flavored liquor. The processing method comprises the following steps: (1) crushing selected sorghum, corn, rice and sticky rice with full particles into 40 meshes of particles by using a crusher and uniformly mixing the mixed particles; (2) wetting the raw materials; (3) pasting the raw materials; (4) putting the raw materials into a pit and sealing by using pit mud to ferment for above 6 months; (5) distilling the fermented raw materials to take out liquor, selecting a liquor jar of 50-65 degrees to age for 3-5 years, blending and obtaining the finished liquor. The processing method has the benefits that as the sorghum, corn, rice and sticky rice are used as the processing raw materials, protein varieties in the raw materials are mutually complementary, so that the growth and the fermentation of microorganisms are promoted; the raw materials processed by using the method can be uniformly wetted; as the raw materials are fully wetted, the complete pasting of the raw materials is facilitated, the utilization rate of the raw materials is improved, and the mouth feeling of the processed liquor is improved.

The present invention provides methods, compositions, and kits for protein crystallization. The present invention involves electrophoretically focusing at least a first protein species within a matrix comprising at least 2 regions of different pH, the protein being present in amount sufficient to permit crystallization within said pH gradient.

The present invention mainly relates to the technical field of agricultural and sideline product processing, and discloses a fermentation and detoxification method of tea seed cake. The method comprises the following steps: crushing, primary fermenting, secondary fermenting, drying and packaging. The method is simple and convenient for large scale operations, does not need heating, is safe and environmentally protective, saves energy, enables the tea seed cake to be completely detoxified, significantly reduces tea saponin residue amount to be as low as 0.03%, ensures safety and non-toxicity, avoids harms on animals, increases added values of the tea seed cake and sesame seed meal, and improves economic income by 9.6%. The sesame seed meal is added into the tea seed cake, and sesamin in thesesame seed meal neutralizes the tea saponin, reduces the content of the tea saponin, balances protein species, promotes microorganism proliferation and speeds up fermentation processes.

The invention relates to a squid protein powder and a preparation method, which is characterized in that it consists of the following raw materials and mass fractions: 35-50 parts of Peruvian squid extract, 90-100 parts of soybean protein isolate, and 25-30 parts of spirulina extract , 10‑12 parts of stachyose, 5‑8 parts of soft white sugar, 0.1‑0.3 parts of citric acid, 3‑5 parts of dextrin, and 10‑15 parts of sodium bicarbonate. The product of the invention is rich in proteins, amino acids, vitamins, minerals and peptides, has high nutritional value, can be taken directly or processed into health care products, leisure foods and other products as raw materials, thereby increasing the use value of the Peruvian squid.