The present invention relates to a nano-Artesunate capsule and a preparation process thereof. A capsule heart is Artesunate, a capsule film is gelatin, a capsule shape is in a ball shape or an ellipsoid shape, and the diameter of the capsule is 30 nm. The invention adopts the following preparation process: swelling the gelatin by distilled water to form colloid, dissolving powdered Artesunate in ethanol at the room temperature, dissolving glacial acetic acid in the distilled water, taking gelatin solution, slowly dropping the Artesunate ethanol solution under the stirring in a water bath with the constant temperature of 40 DEG C, adjusting pH value with glacial acetic acid solution, slowly dropping formaldehyde under the condition of ice bath, solidifying to form the capsule, and freezing and drying to obtain powder of the nano-Artesunate capsule. The capsule prepared by the invention can be taken orally as well as injected by injection, the dosage is small, and no toxic or side effect occurs by taking orally; and the injection cannot cause thrombus, and the like in blood vessels, can support sustained release of medicine delivery, and the injection has high efficiency and long effective period. The grain size of the capsule is uniform, the covering state is good, and the covering rate is 50%; the addition of emulsifier and coagulant aid is not needed during preparation, the process is simple, and the operation is convenient.