The invention refers to microfluidic separation of
plasma for colormetric
assay. A device includes a
plasma separation membrane (110), a
capillary channel (115) positioned adjacent the
plasma separation membrane to receive plasma from blood placed on the plasma separation membrane, at least one
cuvette (125, 245, 250, 255, 260, 332, 334, 336, 338, 342, 344, 346, 348, 420, 425, 430, 435.540, 545, 550, 555, 650, 652, 656) coupled to the
capillary channel, a gas permeable membrane (150, 270, 350, 355, 622), and a distribution channel (120, 230, 235, 240, 325, 330, 525, 530, 545, 630) coupled to the
capillary channel to provide plasma to the
cuvette, wherein the
cuvette is configured to hold an amount of plasma with
reagent suitable for colorimetric
assay by a tester to hold the device. Variations include the use of a quantiation channel (612) to provide a selected amount of plasma and a mixing channel (616) to mix plasma with a
diluent.